Analysis of Chromosome Number in Some Allium and Silene Wild Species with Ornamental Use

Abstract

The present study analyses the number of somatic chromosomes in plant species with ornamental value, in Romanian indigenous flora, Allium (A. flavum L., A. saxatile Bieb.) and Silene (S. compacta Fischer., S. supina M.Bieb.). The biological material was identified and harvested in the South-Eastern part of Dobrogea (Tulcea and Constanţa counties), area in the South-Eastern part of Romania, situated between the Danube and the Black Sea. Individuals from two populations of Allium flavum and Allium saxatile, respectively from Tulcea county (Turcoaia town) and Constanţa county (from Cheile Dobrogei) were analyzed. In the case of Silene compacta and Silene supina, plants of one population in Tulcea county, Turcoaia town, were used. The aim of the analysis was the quantification and comparison of the somatic chromosomes of plants from the same species but different populations, as is the case of the two Allium species, but also to relate them to results from specialty literature, reported by others. The chromosome number was determined in the roots of the small plants that were obtained from seeds collected in their natural habitat. Except the case of Silene supina species, where only diploid forms were identified (2n=24), when in specific literature tetraploid forms are mentioned (2n=48), found in other areas, all other analyzed taxa register similar data to that reported in other geographic regions. Respectively, 2n= 16 in the case of Allium flavum and Allium saxatile and 2n= 24 in the case of Silene compacta.

Molecular and morphological revision of theAllium saxatilegroup (Amaryllidaceae): geographical isolation as the driving force of underestimated speciation

“…In the A. saxatile group, exclusively diploids were found with 2n = 16 chromosomes (Levan, 1935;Vakhtina, 1965Vakhtina, , 1985Tscheschmedjiew, 1973;Gagnidze & Chkheidze, 1975;Magulaev, 1976;Zakirova & Vakhtina in Moore, 1977;Bedalov & Lovrić, 1978;Vakhtina & Kudrjashova, 1978, 1981Van Loon & Kieft in Löve, 1980;Miceli & Garbari, 1980;Pogosian, 1983Pogosian, , 1997Friesen, 1988;Kudrjashova, 1988;Agapova et al, 1990;Özhatay, Koçyigit & Akalın Uruşak, 2012;Draghia et al, 2013). Obligate diploidy is a helpful tool for the correct interpretation of ITS and plastid (chloroplast) DNA sequences.…”