Analysis of Cigarette Smoke Condensates by Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry I Acidic Fraction

Abstract

Cigarette smoke condensate is a complex chemical matrix, and analysis of its components is very difficult because of the limitation of the peak capacity and sensitivity of conventional chromatography and the extensive and laborious sample preparation that is frequently required. In this study, the acidic fraction of mainstream cigarette smoke condensate has been investigated by using comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GCxGC/TOFMS). Different column systems were tested and compared under proper GCxGC/TOFMS conditions. Auto data processing by TOFMS software combined with manual identification was used to assign the components. Over 1000 compounds, with S/N > or = 100, including 139 organic acids and over 150 phenols were tentatively identified by the developed method.

Determination of toxic carbonyl compounds in cigarette smoke

“…There have been numerous reports on the constituents of cigarette smoke (Lu et al, 2003). However, there is no report on MA in the mainstream cigarette smoke prior to the present study.…”

“…Many studies have been conducted to pinpoint the specific chemical(s) that cause these diseases. Consequently, vast numbers of chemicals have been reported in cigarette smoke, over 3000 organic compounds (Lu et al, 2003). In addition, the presence of many toxic chemicals, including polynuclear aromatic hydrocarbons (IARC, 1986), N-nitrosamines (Hecht et al, 1975;Caldwell and Conner, 1990), reactive carbonyl compounds (Miyake and Shibamoto, 1995), dioxins (Muto and Takazawa, 1989), and acrylamide (White et al, 1990) have been recognized in cigarette smoke.…”

Comparison of comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry and gas chromatography–mass spectrometry for the analysis of tobacco essential oils

“…The similarity and reverse factors indicate how well a mass spectrum matches the library spectrum, but isomers have similar mass spectra, so the probability was used to determine whether the peaks with the same name belong to one compound or several compounds. According to the literature [16][17][18], a similarity and reverse number above 800 and 900, a Content is the gravimetric percent of compounds in the essential oil sample. respectively, indicate that an acquired mass spectrum usually shows a good match with the library spectrum.…”

Quantitative determination of five hydroxy acids, precursors of relevant wine aroma compounds in wine and other alcoholic beverages

“…Because of this, derivatization is mostly preferred for hydroxy acid analysis [ 23 , 24 , 27 , 28 , 31 ]. In general, GC MS has been used to analyse these molecules [ 23 , 24 , 27 , 28 , 31 ], although GCxGC/TOF MS has also been used in the analysis of cigarette smoke samples [ 36 ].…”