Analysis of Clinical Isolates of Extended-Spectrum β-Lactamase-Producing Bacteria with Primer and Probe Sets Developed to Detect &lt;i&gt;bla&lt;/i&gt;&lt;sub&gt;CTX-M&lt;/sub&gt;, &lt;i&gt;bla&lt;/i&gt;&lt;sub&gt;TEM&lt;/sub&gt;, and &lt;i&gt;bla&lt;/i&gt;&lt;sub&gt;SHV&lt;/sub&gt; Using a Fully Automated Gene Detection System

Abe, Yoshinobu; Nakamura, Kiwamu; Kaji, Daiki; Takahashi, Hiroshi; Aoki, Kotaro; Kuse, Haruka; Okada, Hideki; Ohta, Kaichi; Ohashi, Kenichi; Takano, Yukiko; Ishii, Yoshikazu; Kanemitsu, Keiji

doi:10.7883/yoken.jjid.2018.466

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The presence of these genes confirmed β-lactamases-producing S. aureus. The presence of five genes in our isolates O11, O19, and H9 indicates that these isolates may produce extended-lactamases and be resistant to vancomycin and methicillin [ 2 , 19 , 31 , 32 ]. Further research is necessary to corroborate the presence of extended-spectrum beta-lactamases-producing S. aureus, VRSA, and MRSA .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance patterns of Staphylococcus aureus isolated from apparently healthy pet cats of Bangladesh

Das,

Kabir,

Chouhan

et al. 2023

J Adv Vet Anim Res

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Objective: This study sought to determine the occurrence, molecular identification, antimicrobi¬al-resistant trends, and gene distribution of Staphylococcus aureus in pet cats and their owners’ hand swabs. Materials and Methods: From different places and clinics in Mymensingh and Dhaka, 168 pet cat samples and 42 hand swab samples from cat owners were obtained. The organisms were scruti¬nized by assessing the outcomes using conventional and molecular techniques. The disc diffusion technique was applied to find the resistance pattern against 12 antibiotics, and genes were dis¬covered by targeting specific genes using PCR. Results: The occurrence of pathogenic S. aureus in pet cats was 7.74%, while it was 9.50% in pet owners’ hand swabs, and 25.0% of the pet owner’s hand swabs contained these genes. Staphylococcus aureus was utterly resistant to amoxicillin, ampicillin, cefixime, erythromycin, and imipenem in both pet cat and hand swabs of pet owner samples. All S. aureus isolates had a multi¬drug-resistant phenotype, and 1 from pet cats (O19) and 1 from pet owner hand swabs (H9) were resistant to all 12 antibiotics in the 7 antimicrobial classes. Several antibiotic-resistance genes were detected by PCR. Conclusion: The study confirmed multidrug-resistant pathogenic S. aureus in pet cats and their owners in Bangladesh, indicating a major health risk to both people and cats. Thus, a holistic and integrated one-health approach between veterinary and medical specialists is needed to mitigate the global distribution of these zoonotic antibiotic-resistant S. aureus strains.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance patterns of Staphylococcus aureus isolated from apparently healthy pet cats of Bangladesh

Das,

Kabir,

Chouhan

et al. 2023

J Adv Vet Anim Res

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“…The sul1 and qnrS genes have been identified in bacteria that are resistant to sulfamethoxazole (sulfonamides) and ciprofloxacin (quinolone's member) antibiotics, respectively (Arsand et al, 2020). According to increasing ARBs and their significant social burden owing to a longer duration of hospitalization, further studies can help to contribute to the identification of general patterns of ARB and ARGs phylogeny associations and identification of spatial and temporal trends of antibiotic resistance distribution (Abe et al, 2019; Pärnänen et al, 2019). Hence, not only virulence but also occurrence and characteristics of ARB in WRRFs should be given enough attention.…”

Section: Introductionmentioning

confidence: 99%

Characterization of antibiotic resistance genes and bacteria in a municipal water resource recovery facility

Rajabi

Farajzadeh

Dehghanzadeh

et al. 2022

Water Environment Research

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Municipal water resource recovery facilities (WRRFs) are important sources of antibiotic‐resistant bacteria and genes (ARB and ARGs). In this study, antibiotic‐resistant total heterotrophic bacteria (THBR) counts (CFU/ml) cultivated from influent, effluent of activated sludge process, and outflow of disinfection unit of an urban WRRF were investigated for the presence of 16, 32, 64, and 128 μg/ml of nine antibiotics. The isolates of Pseudomonas spp., Acinetobacter spp., and Escherichia coli obtained from effluent of activated sludge process were subjected for molecular identification by detecting the 16S rRNA gene sequences. Additionally, using the polymerase chain reaction method (PCR), the isolates were investigated for the presence of blaSHV, blaTEM, blaCTX‐M, blaVIM, sul1, and qnrS genes. According to the results, the abundance of THBR counts was not significantly reduced by the biological treatment except for cefixime and sulfamethoxazole; it also increased for some antibiotics after disinfection unit. The average removal efficiency of THBR resistant to ciprofloxacin, sulfamethoxazole, and ceftazidime were 7.9 ± 1.7%, 41.8 ± 2.1%, and 14.4 ± 6.2%, respectively. Also, all the tested isolates were resistant to at least four antibiotics. For all antibiotics, the resistance ratio (THBR/THB) significantly increased in the effluent and after chlorination unit. Among 12 resistant isolates, blaTEM and sul1 genes were the most frequently detected ones involved in 92% and 83% of the isolates, respectively. Both blaTEM and sul1 genes were found in 100% of E. coli, and 83% and 67% of Pseudomonas spp. isolates, respectively. Further efforts are necessary to limit the transmission of ARB and ARGs from WRRFs into the environment and prevent human health threats. Practitioner Points The ratio of resistance significantly increased after biological treatment. Up to 40% of heterotrophic bacteria in the effluent was antibiotic resistant. blaTEM and sul1 genes were more prevalent (92%) in all isolates of bacteria. Both blaTEM and sul1 genes were found in 100% of E. coli isolates. Pseudomonas spp. holds blaTEM and sul1 genes in 83% and 67% of isolates, respectively.

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Analysis of Clinical Isolates of Extended-Spectrum β-Lactamase-Producing Bacteria with Primer and Probe Sets Developed to Detect <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>TEM</sub>, and <i>bla</i><sub>SHV</sub> Using a Fully Automated Gene Detection System

Cited by 2 publications

References 25 publications

Antimicrobial resistance patterns of Staphylococcus aureus isolated from apparently healthy pet cats of Bangladesh

Antimicrobial resistance patterns of Staphylococcus aureus isolated from apparently healthy pet cats of Bangladesh

Characterization of antibiotic resistance genes and bacteria in a municipal water resource recovery facility

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