Analysis of color properties of raster images of histological microspecimens: own research experience

Ivanova, Mariia; Myroshnychenko, M. S.; Khara, G.I.; Arseniev, Oleksandr V.; Olkhovsky, Vasil; Grygorian, Edgar; Fedulenkova, Yu. Ya.; Козлов, С. В.

doi:10.26641/2307-0404.2022.1.254314

Med. perspekt.

2022

DOI: 10.26641/2307-0404.2022.1.254314

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Analysis of color properties of raster images of histological microspecimens: own research experience

Mariia Ivanova

M. S. Myroshnychenko

G.I. Khara

et al.

Abstract: This study is aimed to develop a computer program to analyze the color properties of raster images of histological microspecimens used in pathological anatomy and forensic medicine. When developing a computer program, we used the system for building client applications – Windows Presentation Foundation (WPF). The system allows you to create applications with visually attractive user interaction capabilities. The programming language is C#, as well as basic graphics capabilities of the .NET Framework system are… Show more

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2024

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Cited by 4 publications

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Features of the claudin 1 and 7 expression in the mucous membrane of the large intestine in symptomatic uncomplicated diverticular disease and acute uncomplicated diverticulitis

Dorohavtseva,

Dorofieiev,

Dyadyk

et al. 2024

GASTRO

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Background. Diverticular disease is one of the most common gastrointestinal pathologies, which mainly damages the large intestine and is manifested morphologically by the formation of sac-like protrusions (diverticula) of the intestinal wall. Today, the etiopathogenesis of diverticular disease remains debatable and needs to be fully understood. Changes in the claudin expression in the colonic mucosa and, accordingly, their structural instability may play a certain role in the development of both diverticular disease and its complications. The study aimed to determine the features of claudin 1 and 7 expression in the colonic mucosa of patients with symptomatic uncomplicated diverticular disease (SUDD) and acute uncomplicated diverticulitis (AUD). Materials and methods. Fragments of the colonic mucosa of 12 individuals without gastrointestinal pathology (group 1), biopsies of the colonic mucosa from the diverticular orifice of 34 patients with SUDD (group 2) and 26 patients with AUD (group 3) were analyzed. An immunohistochemical study used rabbit polyclonal antibodies to claudins 1 and 7. The PAST program was applied to process digital indicators. The average indicators in groups were compared using the Student’s t-test and Mann-Whitney U-test. Results. A comprehensive morphological study revealed a decrease in the expression of claudins 1 and 7 in the colonic mucosa of patients with SUDD and AUD, more pronounced in those with AUD. In the large intestine mucosa of people without gastrointestinal pathology and patients with SUDD and AUD, firstly, the content of claudins 1 and 7 was higher in the surface epithelium compared to the epithelium of the intestinal glands, secondly, the content of claudin 1 predominated over claudin 7 content. Conclusions. The detected changes in claudin 1 and 7 expression in the colonic mucosa biopsies may underlie the development of diverticular disease and its complications.

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Features of the claudin 1 and 7 expression in the mucous membrane of the large intestine in symptomatic uncomplicated diverticular disease and acute uncomplicated diverticulitis

Dorohavtseva,

Dorofieiev,

Dyadyk

et al. 2024

GASTRO

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Morphological Justification of Retromuscular Alloplasty in Diastasis of Rectus Abdominal Muscles

Diadyk,

Koval

2024

Med. Sci. of Ukr.

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Background. Diastasis of the rectus abdominis muscles weakens the white line and contributes to the occurrence of defects with the formation of white line hernias and umbilical hernias. In our opinion, the study of the morphological features of the anterior and posterior aponeurotic walls of the rectus abdominis will make it possible to justify alloplasty, which will increase the effectiveness of surgical treatment of diastasis of the rectus abdominis. Aim: Justification of retromuscular alloplasty of diastasis recti abdominis by studying the morphological features of the anterior and posterior aponeurotic walls of the sheath of the rectus muscle Materials and methods. A comprehensive pathomorphological study of the anterior and posterior aponeurotic walls of the rectus abdominis muscles was conducted in 34 patients (the main group) with diastasis recti who underwent surgical treatment. The comparison group consisted of 32 patients with gallstone disease without diastasis recti who underwent laparoscopic cholecystectomy. The obtained material was examined using histological, histochemical, immunohistochemical, morphometric, statistic methods. Results. In a comparative analysis of the morphological indicators of the front sheet of the sheath of the rectus abdominis muscle with the corresponding indicators of the back sheet in the main group of patients with diastasis of the rectus abdominis, the specific volume of the vascular-fibrous component was more important, the specific volume of adipose tissue and the specific volume the volume of the cavities was less important. The obtained pathomorphological data emphasizes that the front sheet of the sheath of the rectus abdominis muscle in diastasis is mechanically stronger compared to the back sheet. The posterior aponeurotic sheet of the vagina of the rectus muscle in case of diastasis is more prone to ruptures, which is evidenced by the greater value of the specific volume of the cavities in the posterior sheet. Conclusions. Morphological changes of the aponeurotic leaves of the sheath of the rectus abdominis in patients of the main group with diastasis of the rectus muscles in comparison with the comparison group without diastasis differ significantly in the detected changes, the results of the morphological study of the anterior and posterior aponeurotic walls of the rectus muscle in their diastasis indicate the fact that the back walls are thinner, branched , with a decrease in the vascular-fibrous component compared to the front ones, which justifies the strengthening of the back aponeurotic walls by means of retromuscular alloplasty during the surgical treatment of diastasis.

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Morphological and Functional Features of the Mucous Membrane of Small and Large Intestine in Patients With Covid-19 and in Post-Covid-19 Period

Myroshnychenko

Torianyk²,

Arseniev

et al. 2022

Wiad Lek

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The aim: To reveal the morphological and functional features of the mucous membrane of small and large intestine in patients with COVID-19 and in post-COVID-19 period. Materials and methods: In the present study, the authors used biopsy and autopsy material represented by the fragments of the mucous membrane of small and large intestine. All studied material was divided into 10 groups. Group 1 (comparison group) included autopsy material from the deceased who did not have COVID-19 during their lifetime. Groups 2-4 included autopsy material from the deceased who had COVID-19 of varying severity during their lifetime. Groups 5-7 included biopsy material from patients who had recovered from COVID-19 of varying severity, while the duration of the post-COVID period ranged from 1 to 50 days. Groups 8-10 included biopsy material from patients who had in anamnesis COVID-19 of varying severity (the duration of the post-COVID period lasted from 51 to 100 days). Histological, immunohistochemical, morphometric and statistical research methods were used. Results: The comparative analysis showed a more expressed deficiency of ACE2 in the mucous membrane of small and large intestine in patients with moderate and severe COVID-19 compared with patients in post-COVID-19 period of different duration. In patients who had moderate and severe COVID-19 in anamnesis, ACE2 deficiency decreases with increasing duration of post-COVID-19 period. In patients recovered from mild COVID-19, the ACE2 content increases with the duration of post-COVID-19 period from 1 to 50 days and corresponds to the norm with the duration of this period from 51 to 100 days. Conclusions: The comprehensive morphological study conducted by the authors made it possible, firstly, to clarify the morphological and functional features of the mucous membrane of small and large intestine in patients with COVID-19 of various degrees of severity; secondly, to obtain new data about the morpho-functional state of the mucous membrane of small and large intestine in patients, taking into account different duration of the post-COVID-19 period and the severity of the infection.

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Analysis of color properties of raster images of histological microspecimens: own research experience

Cited by 4 publications

References 5 publications

Features of the claudin 1 and 7 expression in the mucous membrane of the large intestine in symptomatic uncomplicated diverticular disease and acute uncomplicated diverticulitis

Features of the claudin 1 and 7 expression in the mucous membrane of the large intestine in symptomatic uncomplicated diverticular disease and acute uncomplicated diverticulitis

Morphological Justification of Retromuscular Alloplasty in Diastasis of Rectus Abdominal Muscles

Morphological and Functional Features of the Mucous Membrane of Small and Large Intestine in Patients With Covid-19 and in Post-Covid-19 Period

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