Analysis of conjugation of chloramphenicol and hemoglobin by fluorescence, circular dichroism and molecular modeling

Ding, Fei; Liu, Wei; Sun, Ye; Yang, Xinling; Sun, Ying; Zhang, Li

doi:10.1016/j.molstruc.2011.10.022

Cited by 25 publications

(7 citation statements)

References 37 publications

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“…Combining these results, the BPEIs do have some interaction with Hb and cause signicant impact on the secondary structure and conformation of Hb. While BPEI is toxic at high concentration, even a polymeric hydrogel of theoretical innite molecular weight yet of basically neutral chains does not signicantly alter the spatial structure of Hb, as reported by Ding et al 17 Therefore, the cytotoxicity including the interaction of a polymer with Hb is polymer dependent. Recently, there have been many reports on the structural and conformational change of plasma proteins such as albumin and brinogen induced by some biopolymers.…”

Section: Spectroscopy Of Hbmentioning

confidence: 89%

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The effect of the gene carrier material polyethyleneimine on the structure and function of human red blood cells in vitro

Li¹,

Zhong²,

Zhang³

et al. 2013

J. Mater. Chem. B

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Section: Spectroscopy Of Hbmentioning

confidence: 89%

“…15 In contrast, while BPEI is toxic at high concentration, even a polymeric hydrogel of theoretical innite molecular weight yet of basically neutral chains does not signicantly alter the spatial structure of Hb. 17…”

Section: Uv-vis Adsorption Spectroscopy Of Hbmentioning

confidence: 99%

The effect of the gene carrier material polyethyleneimine on the structure and function of human red blood cells in vitro

Li¹,

Zhong²,

Zhang³

et al. 2013

J. Mater. Chem. B

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“…Additionally, the previous report showed that isoquercitrin (quercetin-3-O-β-D-glucopyranoside) was one of the bioactive components from numerous medicinal plants that readily interacted with HbS and, as a result, impeded HbS aggregation and polymerization (Syed et al 2019). Using circular dichroism (CD) spectroscopy, Syed et al (2019) revealed that HbS…isoquercitrin complex exhibited helical structural changes leading to destabilization of HbS polymer as previously described (Hamdani et al 2009;Ding et al 2012;Pauline et al 2013) as well as stabilized R-state of HbS. Their findings were in agreement with the proposed mode of action of phytocomponents investigated in the present study.…”

Section: Discussionmentioning

confidence: 63%

Polymerization of deoxygenated sickle hemoglobin in the presence of fractionated leaf extracts of Anacardium occidentale, Psidium guajava, and Terminalia catappa

2020

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Background: The present study evaluated levels of polymerization of deoxygenated sickle hemoglobin molecules (poly-dHbS-M) in the presence of fractionated leaf extracts of Anacardium occidentale Linn., Psidium guajava Linn., and Terminalia catappa Linn in vitro as well as identified, quantified, and characterized the phytocomponents from fractionated leaf extracts that exhibited comparatively high potency to impede poly-dHbS-M. Non-hemolyzed sickle erythrocytes were premixed with 40, 60, and 80 mg/100 mL of each of the separate fractionated leaf extracts of A. occidentale, P. guajava, and T. catappa in phosphate-buffered saline (PBS; pH = 7.4), osmotically equivalent to 9.0 g/ L NaCl. Poly-dHbS-M was induced by adding 2.0 g/100 mL Na 2 S 2 O 5 to the erythrocyte suspension. The absorbance of the erythrocyte suspension was measured at regular intervals of 30 s for 180 s. Identification, quantification, and characterization of phytocomponents from fractionated leaf extracts were carried out using GC-MS, FT-IR, and UVvisible systems protocols. Results: The level of poly-dHbS-M of the control sample was significantly higher (p < 0.05) than those of the samples containing 40, 60, and 80 mg/100 mL ethylacetate extracts of A. occidentale at t < 60 s. The relative cumulative polymerization index (RCPI%) of dHbS-M in the presence of fractionated leaf extract of A. occidentale varied within a wide range of 3.8-59.4%. A. occidentale (petroleum ether and ethylacetate extracts), P. guajava (nhexane, chloroform, and ethylacetate extracts), and T. catappa (ethylacetate extract) exhibited comparatively high potency to inhibit poly-dHbS-M. Conclusion: The fractionated leaf extracts of A. occidentale, P. guajava, and T. catappa exhibited differential capacities to impede poly-dHbS-M. The combinations of aliphatic hydrocarbons, methylated esters, methylated fatty acids, aliphatic alcohols, D-erythro-sphinganine, aromatic derivatives, cycloalkanes, phthalates, isothiocyanates, aminated sugars, cyclo-alcohols, and nitro-compounds impeded poly-dHbS-M.

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“…For the spectrum of Hb-Ca 3 (PO 4 ) 2 •nH 2 O HNFs, the negative peak at 221 nm belongs to the α-helical structure, and the positive peak at 207 nm belongs to the β-turn (corresponding to the π−π* transition) structure. 47,48 This indicates that part of the α-helical structure in Hb is transformed into a β-turn structure during the formation of Hb-Ca 3 (PO 4 ) 2 •nH 2 O HNFs (Figure 2C). The CD spectra of the other six Hb-M 3 (PO 4 ) 2 •nH 2 O HNFs were also analyzed (Figure S13A−F).…”

Section: ■ Experimental Sectionmentioning

confidence: 98%

Controllable Synthesis of Hemoglobin–Metal Phosphate Organic–Inorganic Hybrid Nanoflowers and Their Applications in Biocatalysis

2023

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In recent years, organic–inorganic hybrid nanoflower technology has become an effective method for enzyme immobilization. Here, seven hierarchical flower-like hemoglobin-phosphate organic-inorganic hybrid nanomaterials (Hb-M3(PO4)2 ·nH2O HNFs) were synthesized through an improved universal one-pot wet-chemical method, with Ca2+, Mn2+, Fe2+, Co2+, Ni2+, Cu2+ and Zn2+ as inorganic components. In this synthesis process, the metal cations are successively involved in the coordination reaction with Hb and the metathesis reaction to generate phosphate precipitation. The coordination ability of metal cations and the generation rate of phosphate precipitations were evaluated, then the progress of the two chemical reactions was controlled synchronously by adjusting the phosphate buffer (PB) concentration, and finally a flower-like structure conducive to substrate diffusion and transport was obtained. Due to the conformational transformation of hemoglobin and the abundant Cu2+/Fe3+ active sites, the hemoglobin-Cu3(PO4)2·3H2O nanoflowers have extremely high catalytic activity, which is ∼14 times that of Hb. Importantly, this method is suitable for the monometallic-ionic, polymetallic-ionic and polyvalent metal-ion nanoflowers, which broadens the chemical composition and structural diversity of nanoflowers.

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Analysis of conjugation of chloramphenicol and hemoglobin by fluorescence, circular dichroism and molecular modeling

Cited by 25 publications

References 37 publications

The effect of the gene carrier material polyethyleneimine on the structure and function of human red blood cells in vitro

The effect of the gene carrier material polyethyleneimine on the structure and function of human red blood cells in vitro

Polymerization of deoxygenated sickle hemoglobin in the presence of fractionated leaf extracts of Anacardium occidentale, Psidium guajava, and Terminalia catappa

Controllable Synthesis of Hemoglobin–Metal Phosphate Organic–Inorganic Hybrid Nanoflowers and Their Applications in Biocatalysis

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