Analysis of contribution of collagen fibre component in viscoelastic behaviour of periodontal ligament using enzyme probe

Komatsu, Koichiro; Shibata, Tatsuya; Shimada, Akemi

doi:10.1016/j.jbiomech.2006.12.004

Cited by 14 publications

(11 citation statements)

References 28 publications

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“…Whilst factors such as water content [13] and PDL dimensions are certainly important to the observed behaviour, the response of collagen to the applied stress is central [8]. This is evidenced by studies exposing the PDL to collagenases prior to mechanical characterisation and showing significant increases in the magnitude of stress relaxation following loading [14]. The implications of this behaviour for tooth removal using controlled extrusion is that the PDL is adapted to accumulate damage sub-critically, prior to fibre rupture.…”

Section: Discussionmentioning

confidence: 99%

Extraction force and its determinants for minimally invasive vertical tooth extraction

Thränhardt

Schmid²,

Locher³

et al. 2020

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Discussionmentioning

confidence: 99%

Extraction force and its determinants for minimally invasive vertical tooth extraction

Thränhardt

Schmid²,

Locher³

et al. 2020

Journal of the Mechanical Behavior of Biomedical Materials

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“…Komatsu et al (2007b) investigated into the effect of in vitro digestion of collagen with collagenase on the stress-relaxation process of PDL specimens [77]. The collagenase (8 units/mL)-treated specimens relaxed more rapidly than the control specimens over a 600-second period (Figure 15(a)).…”

Section: Analysis Of Stress-relaxation Response Of the Pdlmentioning

confidence: 99%

“…(a) Stress-relaxation curves as represented by G r ( t ) for specimens treated with PBS or 8 units/mL collagenase, reproduced from Komatsu et al [77], by permission. Measured values G r ( t ) are well expressed by an equation: G r ( t ) = A 1 exp (− t / τ 1 ) + A 2 exp (− t / τ 2 ) + A 3 exp (− t / τ 3 ).…”

Section: Figurementioning

confidence: 99%

Mechanical Strength and Viscoelastic Response of the Periodontal Ligament in Relation to Structure

Komatsu¹

2010

Journal of Dental Biomechanics

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The mechanical strength of the periodontal ligament (PDL) was first measured as force required to extract a tooth from its socket using human specimens. Thereafter, tooth-PDL-bone preparations have extensively been used for measurement of the mechanical response of the PDL. In vitro treatments of such specimens with specific enzymes allowed one to investigate into the roles of the structural components in the mechanical support of the PDL. The viscoelastic responses of the PDL may be examined by analysis of the stress-relaxation. Video polarised microscopy suggested that the collagen molecules and fibrils in the stretched fibre bundles progressively align along the deformation direction during the relaxation. The stress-relaxation process of the PDL can be well expressed by a function with three exponential decay terms. Analysis after in vitro digestion of the collagen fibres by collagenase revealed that the collagen fibre components may play an important role in the long-term relaxation component of the stress-relaxation process of the PDL. The dynamic measurements of the viscoelastic properties of the PDL have recently suggested that the PDL can absorb more energy in compression than in shear and tension. These viscoelastic mechanisms of the PDL tissue could reduce the risk of injury to the PDL.

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“…Based on the imperative role of collagen in the PDL and the efficient activity of collagenase enzymes, the aim of the present study is to investigate for the first time whether the enzymatic disruption of the PDL fibers can actually reduce the force required for tooth extraction. Indeed, collagenase was previously shown to alter the area of collagen fibers in the PDL [ 22 , 28 ]. In this paper, we present an innovative approach to the analytical monitoring of extraction force, which shows clear evidence for a substantial reduction in the force required for tooth extraction following the enzymatic-based disruption of PDL fibers with collagenase in an ex situ model of porcine jaw.…”

Section: Introductionmentioning

confidence: 99%

Collagenase Administration into Periodontal Ligament Reduces the Forces Required for Tooth Extraction in an Ex situ Porcine Jaw Model

Tohar

Alali

Ansbacher

et al. 2022

JFB

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Minimally invasive exodontia is among the long-sought-for development aims of safe dental medicine. In this paper, we aim, for the first time, to examine whether the enzymatic disruption of the periodontal ligament fibers reduces the force required for tooth extraction. To this end, recombinantly expressed clostridial collagenase G variant purified from Escherichia coli was injected into the periodontal ligament of mesial and distal roots of the first and second split porcine mandibular premolars. The vehicle solution was injected into the corresponding roots on the contralateral side. Following sixteen hours, the treated mandibles were mounted on a loading machine to measure the extraction force. In addition, the effect of the enzyme on the viability of different cell types was evaluated. An average reduction of 20% in the applied force (albeit with a large variability of 50 to 370 newton) was observed for the enzymatically treated roots, reaching up to 50% reduction in some cases. Importantly, the enzyme showed only a minor and transient effect on cellular viability, without any signs of toxicity. Using an innovative model enabling the analytical measurement of extraction forces, we show, for the first time, that the enzymatic disruption of periodontal ligament fibers substantially reduces the force required for tooth extraction. This novel technique brings us closer to atraumatic exodontia, potentially reducing intra- and post-operative complications and facilitating subsequent implant placement. The development of novel enzymes with enhanced activity may further simplify the tooth extraction process and present additional clinical relevance for the broad range of implications in the oral cavity.

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Analysis of contribution of collagen fibre component in viscoelastic behaviour of periodontal ligament using enzyme probe

Cited by 14 publications

References 28 publications

Extraction force and its determinants for minimally invasive vertical tooth extraction

Extraction force and its determinants for minimally invasive vertical tooth extraction

Mechanical Strength and Viscoelastic Response of the Periodontal Ligament in Relation to Structure

Collagenase Administration into Periodontal Ligament Reduces the Forces Required for Tooth Extraction in an Ex situ Porcine Jaw Model

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