Analysis of Corynebacterium vaginale by an Immunodiffusion Technique

Smaron, Mary F.; Vice, John L.

doi:10.1128/aem.27.3.469-474.1974

Cited by 3 publications

(6 citation statements)

References 3 publications

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“…It is also clear that this taxospecies has little in common with other genera with which it is occasionally associated. Moreover, the classification of H. vaginalis either in the genus Corynebacterium or in Haemophilus is not supported by antigenic (Vice & Smaron, 1973;Smaron & Vice, 1974) or biochemical (Lapage, 1974;Greenwood & Pickett, 1979) data or, in the former case, by the results of DNA base composition studies (Minnikin et af., 1978). The latter technique can be effective in helping to clarify the relationships between poorly classified strains since bacteria with DNA differing by more than 10 mol % GC should not be classified in the same genus (Bradley & Mordarski, 1976;Bradley, 1980;Priest et al, 1980).…”

Section: Taxonomy Of Gardnerella Vaginalis 389mentioning

confidence: 99%

A Taxonomic Study of Gardnerella vaginalis (Haemophilus vaginalis) Gardner and Dukes 1955

Piot¹,

Dyck

Goodfellow

et al. 1980

Microbiology

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Fifty-five strains received as Haemophilus vaginalis or as catalase-negative coryneform bacteria from the vagina together with 61 marker cultures were subjected to numerical phenetic analyses using 149 unit characters. The data were examined using the simple matching (SSM), Jaccard (SJ) and pattern (DP) coefficients and clustering was achieved using the average linkage algorithm. Cluster composition was not markedly affected by the coefficient used or by test error, estimated at 6 . 5%. The H. vaginalis strains formed a tight cluster which was only distantly related to representatives of the genera arthrobacter, Cellulomonas, Corynebacterium sensu stricto, Erysipelothrix, Haemophilus, Kurthia, Lactobacillus, Listeria and Propionibacterium but shared a high overall affinity to unclassified catalase-negative coryneforms which formed a discrete taxon, cluster 9. The H. vaginalis strains could be distinguished from the related strains in cluster 9 by several unrelated phenotypic characters. Using the S1 endonuclease assay, DNA-DNA hybridizations were performed with representative strains from the numerical as well as with reference strains of Bifidobacterium and Actinomyces. Haemophilus vaginalis was found to be a genotypically legitimate group and its DNA showed little homology with DNA from the marker strains tested. The DNA base composition of H. vaginalis was 42 to 44 mol % guanine plus cytosine. A new genus should be created to incorporate strains known as H. vaginalis or Corynebacterium vaginale. The name Gardnerella vaginalis proposed by Greenwood & Pickett (1979) is supported.

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Section: Taxonomy Of Gardnerella Vaginalis 389mentioning

confidence: 99%

A Taxonomic Study of Gardnerella vaginalis (Haemophilus vaginalis) Gardner and Dukes 1955

Piot¹,

Dyck

Goodfellow

et al. 1980

Microbiology

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“…The subsequent precipitation steps did not result in a significant increase in specific immunological reactivity but were necessary for isolation and separation of antigenic components by DEAE-cellulose chromatography and were included in the remaining experiments. As described previously, three precipitin bands formed when the PCFE preparation was tested for antigenic activity (11). The antigens present in PCFE were designated according to their location with respect to the antibody well: i.e., the antigen that formed the precipitin band closest to the antibody well was designated antigen (i); the antigen that formed the outer precipitin band was designated (o); and the antigen that formed the middle precipitin band was designated (m).…”

Section: Resultsmentioning

confidence: 99%

“…Evaluation of antisera prepared against whole cells and broth extract. The anti-14018Di antiserum used in this study was previously shown to react with all C. vaginale isolates tested by indirect fluorescent-antibody and by Ouchterlony techniques (11,13). Anti-14018Di produces three precipitin bands with PCFE and at least one precipitin band with sonically treated extracts of each of 15 C. vaginale isolates tested and, therefore, was used to evaluate all of the various isolation procedures.…”

Section: Resultsmentioning

confidence: 99%

“…Ouchterlony technique. Specific details of the Ouchterlony technique have been previously reported (11). The concentration of the material tested for antigenic activity varied, depending on the source and technique used to obtain the PCFE.…”

mentioning

confidence: 99%

“…The material from each DEAE fraction was reconstituted with 0.3 ml of deionized water. Two immunodiffusion patterns were routinely used: (i) immunodiffusion systems were prepared in which the fractions were placed in adjacent wells in order to detect patterns of identity and (ii) the fractions were also tested in systems in which they were bordered by wells containing PCFE to enhance the reactions of antigen(s) present in low concentration and, thus, detect their presence (11).…”

mentioning

confidence: 99%

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Immunological and chemical characterization of the extracellular antigens from Corynebacterium vaginale

Smaron¹,

Vice²

1977

Infect Immun

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Supernatants from 72-h peptone-starch-dextrose broth cultures of Corynebacterium vaginale contained significant quantities of three extracellular, soluble antigens (ESA). The ESA were concentrated and partially purified by dialysis followed by ammonium sulfate and ethanol precipitation. Diethylaminoethylcellulose columns were used to isolate two of the three ESA. The ESA were shown to be similar to antigens found on whole C. vaginale cells. Absorption studies indicated that the cell antigens are located at, or near, the surface. On the bases of heat stability, resistance to protease treatment, concanavalin A binding activity, and susceptibility to periodate, it appears that all three ESA are polysaccharide or glycoprotein in nature. A number of serological studies have been undertaken to develop a simple method to distinguish Corynebacterium vaginale and other possibly related bacteria, particularly those found as part of the normal flora of the genital tract (4, 10-13). Although the indirect fluorescent-antibody technique is a simple and rapid method for presumptive identification of C. vaginale (13), it lacks the resolving power of the Ouchterlony technique, which has the ability to recognize antigens and antibodies in mixtures and to establish the identity of antigens. Ouchterlony studies indicate that C. vaginale produces three extracellular, soluble antigens (ESA), at least one of which may be useful to distinguish C. vaginale from other possibly related diphtheroid-like bacteria (11). Interest in the potential use of ESA in techniques for characterization and rapid identification of C. vaginale has prompted our efforts to devise methods for their purification. The present report describes isolation of two of the three antigens from cell-free culture supernatants of C. vaginale. In addition, the three ESA were shown to be carbohydrate or glycoprotein in nature and are immunologically similar to antigens found at, or near, the surface of whole C. vaginale cells. The apparent similarities between ESA of C. vaginale and the C-polysaccharide and C-capsular polysaccharide of Streptococcus pneumoniae are discussed. MATERIALS AND METHODS Organisms. The type strain 594 was obtained from the American Type Culture Collection (14018). Media. C. vaginale was isolated and maintained on blood agar plates (Baltimore Biological Laboratory, Cockeysville, Md.), incubated under increased carbon dioxide tension in a candle jar at 37°C. In the first series of experiments, C. vaginale was

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Humoral circulatory immune response to Gardnerella vaginalis

et al. 1989

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Strain-specific circulating immunoglobulin G and/or M was detected by enzyme-linked immunosorbent assay and immunofluorescence test by using Formol-treated suspensions of Gardnerella vaginalis from 28 women with overt vaginitis but only three symptom-free subjects among 43 otherwise healthy women found to be colonized by G. vaginalis. Analogous but less stringent strain specificity patterns were elicited by immunization of BALB/c mice.

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Analysis of Corynebacterium vaginale by an Immunodiffusion Technique

Cited by 3 publications

References 3 publications

A Taxonomic Study of Gardnerella vaginalis (Haemophilus vaginalis) Gardner and Dukes 1955

A Taxonomic Study of Gardnerella vaginalis (Haemophilus vaginalis) Gardner and Dukes 1955

Immunological and chemical characterization of the extracellular antigens from Corynebacterium vaginale

Humoral circulatory immune response to Gardnerella vaginalis

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