Analysis of cutaneous bacterial microbiota of Thai patients with seborrheic dermatitis

Abstract

Seborrheic dermatitis (SD) is a chronic inflammatory skin condition that occurs in body areas that contain profuse sebaceous glands. Skin microbiota are diverse across ethnic groups and its dysbiosis has been implicated in the pathogenesis of SD. Here, we reported the contribution of cutaneous bacterial microbiota to SD in the Thai population. Healthy individuals and patients with scalp SD were recruited into the study. Normal skin, scalp skin lesion (SL) and non‐lesion sites (SNL) samples were collected using a tape stripping method and next‐generation sequencing of 16S rRNA for microbiome analysis. Although bacterial diversity in all sample groups was not statistically different, a population of bacteria commonly found on skin of scalp showed signs of dysbiosis. Apart from the reduction of Corynebacterium spp., SD‐specific microbiota was dominated by Firmicutes at taxa level and Pseudomonas spp., Staphylococcus spp. and Micrococcus spp. at genus level. The dysbiosis of the skin microbiota in SD was specifically described as an alteration of bacteria populations commonly found on scalp skin, implying that managing and controlling the cutaneous bacterial microbiome can alleviate and prevent SD and pave the way for the development of new SD treatments.

Treatment with the Topical Antimicrobial Peptide Omiganan in Mild-to-Moderate Facial Seborrheic Dermatitis versus Ketoconazole and Placebo: Results of a Randomized Controlled Proof-of-Concept Trial

“…Previous studies in atopic dermatitis patients demonstrated a clear staphylocidal effect and decreased microbial dysbiosis compared to the placebo with doses as low as 1% once or twice daily [ 23 , 24 ]. Involvement of Staphylococcus , and especially S. aureus , in the pathophysiology of atopic dermatitis has gained attention [ 6 , 7 ], and microbial profiling studies in SD have shown increased Staphylococcus abundances, accordingly [ 5 , 31 , 32 , 33 ]. However, this might not necessarily constitute dysbiosis as these studies have shown similar [ 32 , 33 ] or higher [ 31 ] biodiversity indexes compared to healthy controls.…”

“…Involvement of Staphylococcus , and especially S. aureus , in the pathophysiology of atopic dermatitis has gained attention [ 6 , 7 ], and microbial profiling studies in SD have shown increased Staphylococcus abundances, accordingly [ 5 , 31 , 32 , 33 ]. However, this might not necessarily constitute dysbiosis as these studies have shown similar [ 32 , 33 ] or higher [ 31 ] biodiversity indexes compared to healthy controls. Moreover, this study was limited by insufficient phylogenetic resolution, which does not enable differentiation between S. aureus and S. epidermidis that are regarded as pathobiont and commensal species, respectively [ 34 ].…”

Non-significant influence between aerobic and anaerobic sample transport materials on gut (fecal) microbiota in healthy and fat-metabolic disorder Thai adults

“…PCR amplification of the 16S rRNA gene at the V3-V5 region was performed using the universal prokaryotic primers 342F (5′-GGRGGCAGCAGTNGGGAA-3′) and 895R (5′-TGCGDCCGTACTCCCCA-3′) with appended barcode and adaptor sequences ( Human Microbiome Project (HMP) Consortium, 2012a ; Castelino et al, 2017 ; Wongsaroj et al, 2021 ; Dityen et al, 2022 ). The 342F was used elsewhere and the 895R position was shared with the 909R.…”

“…The 342F was used elsewhere and the 895R position was shared with the 909R. The in-silico analysis revealed that the V3-V5 primers could identify bacteria on phylum/class/order/family levels with >77% efficiency, genus 56.6% and species 21.1% ( Wang & Qian, 2009 ; Human Microbiome Project (HMP) Consortium, 2012a ; Castelino et al, 2017 ; Johnson et al, 2019 ; Darwish et al, 2021 ; Suwarsa et al, 2021 ; Wongsaroj et al, 2021 ; Dityen et al, 2022 ). Each PCR reaction comprised 1 × EmeraldAmp GT PCR Master Mix (TaKaRa, Shiga, Japan), 0.2 μM of each primer, and 50–100 ng of the genomic DNA in a total volume of 75 µL.…”

“…Then, the ~640-base pair (bp) amplicon was excised from agarose gel resolution and purified using PureDireX PCR Clean-Up & Gel Extraction Kit (Bio-Helix, Keelung, Taiwan), and quantified using a Qubit 3.0 Fluorometer and Qubit dsDNA HS Assay kit (Invitrogen, Waltham, MA, USA). Finally, 180 ng of each barcoded amplicon product was pooled for sequencing using the Miseq600 platform (Illumina, San Diego, CA, USA), along with the sequencing primers and index sequence ( Caporaso et al, 2012 ; Wongsaroj et al, 2021 ; Dityen et al, 2022 ; Ondee et al, 2022 ), at the Omics Sciences and Bioinformatics Center, Chulalongkorn University (Bangkok, Thailand).…”