Analysis of cytochrome c-oxidase (COI) gene of mitochondrial DNA from the Trichinella spp. in China

Yang, Yurong; Jian, Wei; Pozio, Edoardo

doi:10.1007/s00436-008-1141-y

Cited by 2 publications

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“…In agreement with previous studies (Yang et al, 2008; Fu et al, 2009; Wang et al, 2012), the taxonomic position of Chinese Trichinella belongs to T. spiralis , which was unambiguously supported in the phylogenetic analysis. Although the exact divergence pattern of Chinese isolates was unresolved, the ML method supported two clades, HLJ-hb + HN-ny + YN-dl and GX-td + HB-xf + Tibet-lz.…”

Section: Discussionsupporting

confidence: 91%

“…Although great achievements have been made in the taxonomy, phylogeny, and biogeography of the Trichinella genus during the last decade (Zarlenga et al, 2006; Pozio et al, 2009; Mohandas et al, 2014; Korhonen et al, 2016), only a few studies have focused on the genetic diversity of the Chinese T. spiralis population. As described above, human trichinellosis cases have been recorded in 17 provinces/autonomous regions/municipalities of China; only seven geographical isolates from the endemic regions were identified (Yang et al, 2008; Fu et al, 2009; Wang et al, 2012; Zhang et al, 2016). Furthermore, all of the previous studies performed the genetic analysis of different Trichinella isolates either by few or even single molecular marker, or with insufficient sample size.…”

Section: Introductionmentioning

confidence: 99%

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Genotyping and Phylogenetic Position of Trichinella spiralis Isolates from Different Geographical Locations in China

et al. 2019

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In China, the nematode Trichinella spiralis is the main aetiological agent of human trichinellosis. We performed multi-locus microsatellite typing of T. spiralis isolates to improve the current knowledge of the evolution and population diversity. First, seven polymorphic microsatellite loci were used to infer the genetic diversity of T. spiralis collected in 10 endemic regions. Then, a Bayesian model-based STRUCTURE analysis, a clustering based on the neighbor-joining method, and a principal coordinate analysis (PCA) were performed to identify the genetic structure. Finally, the phylogenetic position of Chinese isolates was explored based on six mitochondrial and nuclear genetic markers (cox1, cytb, 5S ISR, ESV, ITS1, and 18S rDNA) using the maximum likelihood and Bayesian methods. In addition, the divergence time was estimated with multiple genes using an uncorrelated log-normal relaxed molecular-clock model. A total of 16 alleles were detected in 2,310 individuals (1,650 muscle larvae and 660 adult worms) using seven loci. The STRUCTURE analysis indicated that the T. spiralis isolates could be organized and derived from the admixture of two ancestral clusters, which was also substantiated through the clustering analysis based on the allelic data. PCA separated most samples from Tiandong, Guangxi (GX-td), and Linzhi, Tibet (Tibet-lz), from the remaining isolates. However, both maximum likelihood and Bayesian inference supported the close relationship between Xiangfan, Hubei (HB-xf), and GX-td. The molecular dating analysis suggested that the Chinese isolates started to diverge during the Late Pleistocene (0.69 Mya). Generally, T. spiralis was observed to harbor low genetic variation, and further investigation with deeper sampling is needed to elucidate the population structure.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Genotyping and Phylogenetic Position of Trichinella spiralis Isolates from Different Geographical Locations in China

et al. 2019

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Identification of astigmatid mites using ITS2 and COI regions

2010

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Identification of astigmatid mites based on their morphological characteristics is difficult because of the similarity of their organs, especially in immature mites. The ribosomal second internal transcribed spacer (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) regions are highly conserved in the eukaryotes and are usually used as barcodes. The ITS2 and COI regions of six species of astigmatid mites (Aleuroglyphus ovatus, Blomia tropicalis, Dermatophagoides farinae, Dermatophagoides pteronyssinus, Euroglyphus maynei, Tyrophagus putrescentiae) were obtained by polymerase chain reaction and sequenced. The lengths of the ITS2 sequences varied from 316 to 488 bp, while the COI regions were 377 or 378 bp long. Considering the ITS2 genes, the intraspecific genetic distance was in the range of 0.00-0.077844, whereas the interspecific genetic distance was 0.202426-0.912959. The values were 0.000-0.029748 and 0.138403-0.279304 for intra- and interspecific genetic distances when COI genes were used. The phylogenetic trees inferred from the ITS2 and the COI regions, by using maximum parsimony and neighbor-joining methods, were identical to those based on their morphological classification. Thus, the ITS2 and COI regions can be applied as barcodes to identify different species of astigmatid mites.

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Analysis of cytochrome c-oxidase (COI) gene of mitochondrial DNA from the Trichinella spp. in China

Cited by 2 publications

References 36 publications

Genotyping and Phylogenetic Position of Trichinella spiralis Isolates from Different Geographical Locations in China

Genotyping and Phylogenetic Position of Trichinella spiralis Isolates from Different Geographical Locations in China

Identification of astigmatid mites using ITS2 and COI regions

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