Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies

Abstract

To identify the enzymes potentially useful for the decolorization of azo dyes, the secretome of the ascomycetous fungus Myrothecium roridum IM6482 was studied by using a bottom-up proteomic approach. Among the identified proteins, the most promising for dye removal was laccase, which decolorized respectively, 66, 91, 79, and 80% of Acid Blue 113 (AB 113), Acid Red 27 (AR 27), Direct Blue 14 (DB 14), and Acid Orange 7 (AO 7). The degradation of dyes was enhanced at the wide range of pH from 4 to 8. The addition of redox mediators allowed eliminating AB 113 in concentrations up to 400 mg/L and decolorization of the simulated textile effluent. Microbial toxicity and phytotoxicity tests indicated that dyes are converted into low-toxicity metabolites. This is the first insight into the M . roridum secretome, its identification and its application for removal of select azo dyes. Obtained results extended knowledge concerning biodegradative potential of ascomycetous, ligninolytic fungi and will contribute to the improvement of dye removal by fungi. Electronic supplementary material The online version of this article (10.1007/s11356-019-05324-6) contains supplementary material, which is available to authorized users.

Bisphenol A Removal by the Fungus Myrothecium roridumIM 6482—Analysis of the Cellular and Subcellular Level

“…The present work demonstrates a response to BPA of the ascomycetous fungus M. roridum IM 6482, previously described as an effective dye degrader and laccase producer [24][25][26]. The fungus was isolated from a postindustrial area and successfully used for the elimination of dye.…”

“…The growth and degradability of BPA by the ascomycetous fungus M. roridum IM 6482 was assessed in Czapek-Dox cultures containing 50 mg·L −1 of BPA. The M. roridum strain had been previously described as a producer of copper-induced laccase capable of decolorization of dyes [ 24 , 25 , 26 ]. Therefore, fungal growth and biodegradation of BPA by M. roridum IM 6482 was also investigated in the presence of copper, which is a commonly known laccase inducer.…”

Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain Pleurotus eryngii

“…Jasi ńska et al studied the degradation of azo dyes using a crude laccase preparation from Myrothecium roridum. The introduction of a synthetic or natural mediator to a reaction mixture containing 1 U/mL laccase and the azo dye AB 113 resulted in a significant enhancement of the decolorization efficiency, indicating that appropriate mediator supplementation could promote the degradation of substrates that may be less amenable to laccase-mediated degradation [35]. Anthraquinone dyes contain an anthraquinone unit in their molecules, including anthraquinone derivatives and fused-ring ketone dyes produced from anthraquinone derivatives.…”

An Integrative Approach to Study Bacterial Enzymatic Degradation of Toxic Dyes

“…On the other hand, microorganisms are favored more for the decolorization process because of their unique metabolic pathways and protein frameworks that help them mineralize and decolorize the dye completely under specific ecological conditions. The microorganisms that decolorize various azo dyes are fungi (Bankole et al, 2018;Jasińska et al, 2019;Usha et al, 2020), algae (Mahalakshmi et al, 2015;Raymond and Kadiri, 2017;Ishchi and Sibi, 2020), yeast (Yu and Wen, 2005;Mahmoud, 2014), and bacteria (Hossen et al, 2019). Microbial degradation of azo dye occurs under aerobic, anaerobic, or microaerophilic conditions.…”