Analysis of dexamethasone sodium phosphate formulations by high-performance liquid chromatography

Gagné, Denise R; Lodge, Bruce A.

doi:10.1016/s0021-9673(00)81459-4

Cited by 12 publications

(8 citation statements)

References 2 publications

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“…These include HPLC (Gagne & Lodge, 1980; Lamiable et al , 1986; Charles et al , 1993; Schild & Charles, 1994), various antibody‐associated immunoassays (Chen et al , 1996; Ribeiro Neto et al , 1997), and methodologies utilizing multiple detection devices (Friedrich et al , 1992; Gu et al , 1996). The lowest documented quantifiable dexamethasone concentrations in plasma and urine in these investigations was 0.01 ng/mL (Gagne & Lodge, 1980; Derendorf et al , 1986; Lamiable et al , 1986; Friedrich et al , 1992; Chen et al , 1996; Gu et al , 1996; Ribeiro Neto et al , 1997). Sample volumes achieving this level of quantification, however, equaled or exceeded 1 mL, whereas the starting sample volume in the current investigation equaled 0.1 mL.…”

Section: Discussionmentioning

confidence: 99%

“…Sample preparation and analysis methods for the simultaneous extraction and determination of dexamethasone and dexamethasone‐phosphate were derived from previous investigations analyzing these drugs separately (Gagne & Lodge, 1980; Lamiable et al , 1986; Charles et al , 1993). Sample extractions, chromatographic separation, and determination were conducted at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

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Iontophoresis of dexamethasone‐phosphate into the equine tibiotarsal joint

Blackford

Doherty

Ferslew

et al. 2000

Vet Pharm & Therapeutics

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In human rehabilitation medicine, dexamethasone-phosphate is theoretically iontophoresed to localized subcutaneous tissue where conversion to dexamethasone occurs. This delivery system has recently been introduced into veterinary medicine for the same purpose. However, the pharmacokinetic justification for parenteral delivery of this prodrug remains undocumented. Utilizing iontophoretic methods that are relevant to both human and veterinary clinical practice, the present investigation compared injection and iontophoresis of dexamethasone-phosphate into the equine tibiotarsal joint, also known as the tarsocrual joint. The tibiotarsal joints of seven horses were injected with 4 mL of 6 mg/mL dexamethasone-phosphate. With a similar drug concentration and over the same application site, six different horses underwent simultaneous cathodic iontophoresis (4 mA, 40 min) or passive application (0 mA, 40 min) on contralateral limbs. Following all applications, tibiotarsal joint synovium was collected. Local venous blood samples were also collected from the iontophoretic and passive application sites for analysis of plasma drug concentrations. Because of the potential for conversion of dexamethasone-phosphate to dexamethasone, an extraction and analysis protocol was developed for both chemicals. The technique demonstrated a linear range of detection (0.39-12 microg/mL) and a capability for measuring both chemicals in plasma and synovium. Conversion of dexamethasone-phosphate to dexamethasone occurred during synovial incubation (37 degrees C) and following freeze-thaw cycles. In contrast to the measurable synovial concentrations of dexamethasone-phosphate (2.3 +/- 0.96 mg/mL) and dexamethasone (0.27 +/- 0.07 mg/mL) following injection, neither drug was detected in the synovium or the local venous blood following iontophoretic or passive applications. In conclusion, these results do not confirm iontophoretic or passive delivery of measurable dexamethasone-phosphate into the tibiotarsal joint using current clinical methods.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Iontophoresis of dexamethasone‐phosphate into the equine tibiotarsal joint

Blackford

Doherty

Ferslew

et al. 2000

Vet Pharm & Therapeutics

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“…Mixing drugs will prevent multiple perfusions. The stability of single drugs has also been reported by numerous authors (3)(4)(5)(19)(20)(21). Most of the stability studies have been carried out on binary drug mixtures (9)(10)(11)(12)(13)(14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 87%

“…HPLC methods for determining the drugs involved in this study have already been reported (3)(4)(5)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23) …”

Section: Introductionmentioning

confidence: 99%

Hplc–dad Method for Studying the Stability of Solutions Containing Morphine, Dexamethasone, Haloperidol, Midazolam, Famotidine, Metoclopramide, and Dimenhydrinate

Nassr

Brunet

Lavoie³

et al. 2001

Journal of Liquid Chromatography & Related Technologies

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The objectives of this study were to evaluate both the compatibility and the stability of morphine when mixed with different drugs and to provide recommendations for appropriate conservation conditions. Five drug mixtures used for palliative care were stored in polypropylene syringes at different temperatures (25 • C, 4 • C) up to 96 h. These mixtures were: 1) ORDER REPRINTS 266NASSR ET AL.Haloperidol, Famotidine, Metoclopramide, Dimenhydrinate. Drug mixtures were prepared in NaCl 0.9%, in order to obtain a 100 mL final solution containing the maximum daily dose of each component. For the separation and quantification of active ingredients, a fast, precise, accurate, and sensitive method was developed. Drugs were separated using HPLC-DAD (High performance liquid chromatography-diode array detector) with a Zorbax Eclipse XDB C 18 ) column under elution gradient. Just after preparing the mixture of drugs and then after 4,8,12,24, 48, 72, 96 h, the physical appearance of each solution was observed, and drug concentrations were controlled. Stability was assumed if the loss after 96 h was less than 10% of the initial concentration. Mixture number (2) was incompatible when drugs were mixed. Mixing haloperidol and dexamethasone gave rise to the formation of a white precipitate. Mixture (3) was stable and compatible at 25 • C, but incompatible at 4 • C due to crystallization of haloperidol. All the other drug mixtures were stable and compatible both at 4 • C and 25 • C for 96 h.

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“…Gagne et al also described a LC method for the analysis of dexamethasone sodium phosphate in formulations such as ophthalmic solutions, injectable preparations and inhalant formulations [21]. For the simultaneous determination of trimethoprim, dexamethasone sodium phosphate and polymyxin B sulphate, a method using micellar electrokinetic capillary chromatography was developed by Gallego and Perez Arroyo [22].…”

Section: Introductionmentioning

confidence: 99%