Analysis of Different Natural and Synthetic Biomaterials to Support Cardiomyocyte Growth

Kerkelä, Erja; Kujala, Kirsi; Poh, Tan Lay; Kellomäki, Minna; Haaparanta, Anne-Marie; Ahola, Niina; Ellä, Ville; Ikonen, Liisa; Aalto‐Setälä, Katriina

doi:10.4172/2155-9880.s4-002

Cited by 7 publications

(10 citation statements)

References 35 publications

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“…In general, collagen hydrogels have been shown to support cardiomyocyte survival and formation of a 3D network within the hydrogels. 59 Taken together, our results suggest that (a) collagen concentration plays an important role in cardiomyocyte survival, with higher concentration possibly resulting in less porosity, thereby decreasing cell survival and (b) mixing 2 mg mL 21 collagen scaffolds with ECM proteins might not offer additional benefit to cardiomyocyte survival.…”

Section: Resultsmentioning

confidence: 63%

“…Evans et al noted similar effects of fibronectin and laminin substrates on embryonic and neonatal cardiomyocyte survival wherein, 2D fibronectin substrates and collagen type‐I (2D substrates or 3D scaffolds) provided greater than 85% cell survival, while 2D laminin substrate allowed for only 55% cell survival. In general, collagen hydrogels have been shown to support cardiomyocyte survival and formation of a 3D network within the hydrogels . Taken together, our results suggest that (a) collagen concentration plays an important role in cardiomyocyte survival, with higher concentration possibly resulting in less porosity, thereby decreasing cell survival and (b) mixing 2 mg mL − 1 collagen scaffolds with ECM proteins might not offer additional benefit to cardiomyocyte survival.…”

Section: Resultsmentioning

confidence: 65%

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Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes

Gishto

Farrell

Kothapalli

2014

J Biomedical Materials Res

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A major onset of heart failure is myocardial infarction, which causes the myocardium to lose cardiomyocytes and transform into a scar tissue. Since mammalian infarcted cardiac tissue has a limited ability to regenerate, alternative strategies including implantation of tissue-engineered scaffolds at the site of damaged myocardium have been explored. The goal is to enable in situ cardiac reconstruction at the injured myocardium site, replace the lost cardiomyocytes, deliver the required biomolecules, and remodel the extracellular matrix (ECM). ECM synthesis and deposition by cardiomyocytes within such scaffolds remains categorically unexplored. Here, we investigated the survival, ECM synthesis and deposition, and matrix metalloproteinases (MMPs) release by cardiomyocytes within three-dimensional (3D) substrates. Rat cardiomyocytes were cultured for three weeks within two structurally different substrates: 3D collagen hydrogels or polycaprolactone (PCL) nanofibrous scaffolds. The concentration and composition of the hydrogels was varied, while PCL nanofibers were surface-modified with various ECM proteins. Results showed that myocyte attachment and survival was higher within collagen hydrogels, while myocyte alignment and beating was noted only within PCL scaffolds. Total protein synthesis by myocytes within PCL scaffolds was significantly higher compared to that within collagen hydrogels, although more protein was deposited as matrix within hydrogels. Significant ECM synthesis and matrix deposition, TIMP-1, and MMP release were noted within modified collagen hydrogels and PCL nanofiber scaffolds. These results were qualitatively confirmed by imaging techniques. Results attest to the prominent role of scaffold composition and architecture in influencing cardiomyocyte phenotype, matrix synthesis and cytokines release, with significant applications in cardiac tissue remodeling strategies.

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Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 65%

Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes

Gishto

Farrell

Kothapalli

2014

J Biomedical Materials Res

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“…In our previous study [14], we showed that the growth of NRCs was best supported by natural collagen. Therefore, we wanted to continue our studies using a material with properties similar to those of natural collagen.…”

Section: Discussionmentioning

confidence: 99%

“…Scaffold-free human cardiac tissue has also been generated [13]. In our previous studies, we compared different synthetic and natural biomaterials for their ability to support cardiomyocyte growth [14]. Of the biomaterials we tested, natural collagen supported cardiomyocyte growth the best but showed significant batch-to-batch variation.…”

Section: Introductionmentioning

confidence: 99%

“…Although PuraMatrix was shown to be less effective than collagen at supporting the growth and survival of cardiomyocytes, it performed well enough to attract our interest in this class of materials [14]. We previously reported the development of self-assembling nanofiber systems with a modular architecture based on a 1,3,5-triamide cis,cis-cyclohexane core [15].…”

Section: Introductionmentioning

confidence: 99%

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2D and 3D Self-Assembling Nanofiber Hydrogels for Cardiomyocyte Culture

Ikonen

Kerkelä

Metselaar³

et al. 2013

BioMed Research International

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Collagen is a widely used biomaterial in cardiac tissue engineering studies. However, as a natural material, it suffers from variability between batches that can complicate the standardization of culture conditions. In contrast, synthetic materials are modifiable, have well-defined structures and more homogeneous batches can be produced. In this study, several collagen-like synthetic self-assembling nanofiber hydrogels were examined for their suitability for cardiomyocyte culture in 2D and 3D. Six different nanofiber coatings were used in the 2D format with neonatal rat cardiomyocytes (NRCs) and human embryonic stem-cell-derived cardiomyocytes (hESC-CMs). The viability, growth, and functionality of the 2D-cultured cardiomyocytes were evaluated. The best-performing nanofiber coatings were selected for 3D experiments. Hydrophilic pH-sensitive nanofiber hydrogel coassembled with hyaluronic acid performed best with both NRCs and hESC-CMs. Hydrophilic non-pH-sensitive nanofiber hydrogels supported the growth of NRCs; however, their ability to promote attachment and growth of hESC-CMs was limited. NRCs also grew on hydrophobic nanofiber hydrogels; however, the cell-supporting capacity of these hydrogels was inferior to that of the hydrophilic hydrogel materials. This is the first study demonstrating that hydrophilic self-assembling nanofiber hydrogels support the culture of both NRCs and hESC-CMs, which suggests that these biomaterials hold promise for cardiac tissue engineering.

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3D Culture Modeling of Metastatic Breast Cancer Cells in Additive Manufactured Scaffolds

Nanou

Lorenzo-Moldero

Gazouleas

et al. 2022

ACS Appl. Mater. Interfaces

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Cancer biology research is increasingly moving toward innovative in vitro 3D culture models, as conventional and current 2D cell cultures fail to resemble in vivo cancer biology. In the current study, porous 3D scaffolds, designed with two different porosities along with 2D tissue culture polystyrene (TCP) plates were used with a model breast cancer human cell line. The 3D engineered system was evaluated for the optimal seeding method (dynamic versus static), adhesion, and proliferation rate of MDA-MB-231 breast cancer cells. The expression profiles of proliferation-, stemness-, and dormancy-associated cancer markers, namely, ki67, lamin A/C, SOX2, Oct3/4, stanniocalcin 1 (STC1), and stanniocalcin 2 (STC2), were evaluated in the 3D cultured cells and compared to the respective profiles of the cells cultured in the conventional 2D TCP. Our data suggested that static seeding was the optimal seeding method with porosity-dependent efficiency. Moreover, cells cultured in 3D scaffolds displayed a more dormant phenotype in comparison to 2D, which was manifested by the lower proliferation rate, reduced ki67 expression, increased lamin A/C expression, and overexpression of STCs. The possible relationship between the cell affinity to different extracellular matrix (ECM) proteins and the RANK expression levels was also addressed after deriving collagen type I (COL-I) and fibronectin (FN) MDA-MB-231 filial cell lines with enhanced capacity to attach to the respective ECM proteins. The new derivatives exhibited a more mesenchymal like phenotype and higher RANK levels in relation to the parental cells, suggesting a relationship between ECM cell affinity and RANK expression. Therefore, the present 3D cell culture model shows that cancer cells on printed scaffolds can work as better representatives in cancer biology and drug screening related studies.

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Analysis of Different Natural and Synthetic Biomaterials to Support Cardiomyocyte Growth

Cited by 7 publications

References 35 publications

Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes

Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes

2D and 3D Self-Assembling Nanofiber Hydrogels for Cardiomyocyte Culture

3D Culture Modeling of Metastatic Breast Cancer Cells in Additive Manufactured Scaffolds

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