2002
DOI: 10.1095/biolreprod66.3.589
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Analysis of Differential Messenger RNA Expression Between Bovine Blastocysts Produced in Different Culture Systems: Implications for Blastocyst Quality1

Abstract: Using reverse transcriptase-amplified fragment length polymorphism (RT-AFLP) analysis of differential mRNA expression and semiquantitative reverse transcriptase-polymerase chain reaction, we compared mRNA expression in bovine blastocysts from 4 sources, known to differ in quality in terms of their ability to withstand cryopreservation: 1) in vitro culture in synthetic oviduct fluid of in vitro-matured (IVM)/in vitro fertilized (IVF) zygotes; 2) in vitro culture in TCM-199 supplemented with granulosa cells (coc… Show more

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Cited by 305 publications
(246 citation statements)
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“…In case of HMC-derived blastocysts reconstructed from mature oocytes of different resveratrol treatment groups, expression of a set of genes which are having relevance in early embryonic development [13,25,35,42,43] were checked. The genes included in the present study are involved in pre-and postnatal growth (IGF -1 ), metabolism (Glut1 ), pluripotency (Oct4), morphogenesis and biogenesis of the trophoectoderm (E -cad), lineage determination and cavitation (Stat3), gap junction, cavitation and cryosurvival (Cx43), growth arrest (CHOP-10), oxidative stress (MnSOD), and DNA methylation (DNMT).…”
Section: Discussionmentioning
confidence: 99%
“…In case of HMC-derived blastocysts reconstructed from mature oocytes of different resveratrol treatment groups, expression of a set of genes which are having relevance in early embryonic development [13,25,35,42,43] were checked. The genes included in the present study are involved in pre-and postnatal growth (IGF -1 ), metabolism (Glut1 ), pluripotency (Oct4), morphogenesis and biogenesis of the trophoectoderm (E -cad), lineage determination and cavitation (Stat3), gap junction, cavitation and cryosurvival (Cx43), growth arrest (CHOP-10), oxidative stress (MnSOD), and DNA methylation (DNMT).…”
Section: Discussionmentioning
confidence: 99%
“…The ability of in vitro produced embryos to survive cryopreservation, an indicator of embryo quality [4,5,8,12,41,42], is affected by culture conditions. Thus, Dinnyés et al [29] reported high survival rates (85% and 90%) after vitrification/warming of Day 8 expanded embryos produced in SOF without and with FCS, respectively; the hatching rates the above authors obtained for embryos produced in SOF + FCS (63%) are comparable to the survival rates in our work in the Vero and BSA groups.…”
Section: Discussionmentioning
confidence: 99%
“…The freezing sensitivity should be considered as a factor affecting the quality of the in vitro produced bovine blastocysts [10][11][12][13]. Improvement of embryo survival after freezing can be achieved by changing the conditions of in vitro culture, and/or modifying ''standard'' cryopreservation procedures [14].…”
Section: Introductionmentioning
confidence: 99%
“…These early deviations in gene expression patterns seen in NT-derived embryos warrant further investigations in post-implantation and neonatal development to understand the causative mechanism of the abnormalities and elevated mortality after transfer of NT-and activation-derived embryos (Daniels et al, 2001;Wrenzycki et al, 2001;Humpherys et al, 2002). The change of these genes mRNA directly affects the quality of embryos (Rizos et al, 2002;Ruddock et al, 2004). By immunohistochemistry, BDNF protein is localized in trophectoderm cells.…”
Section: Discussionmentioning
confidence: 99%