Analysis of Differentially Expressed Genes Involved in Stevioside Biosynthesis in Cultures of Stevia Rebaudiana Bertoni Treated with Steviol as an Immediate Precursor

Modi, Arpan; Raj, Sandip; Kanani, Poonam; Patel, Armi; Subhash, Narayanan

doi:10.1007/s00344-013-9396-7

Cited by 9 publications

(2 citation statements)

References 13 publications

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“…(2011) were also utilised in several studies to isolate RNA from stevia species (Madhav et al . 2013; Modi et al . 2014; Michalec-Warzecha et al .…”

Section: Discussionmentioning

confidence: 99%

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Efficient and High-Quality RNA Isolation from Metabolite-Rich Tissues of Stevia rebaudiana, an Important Commercial Crop

Leh

Yong

Nulit

et al. 2019

tlsr

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Stevia rebaudiana , a perennial herb native to northeastern Paraguay, has gained immense attention globally over the recent decades due to the natural sweetness of its leaves. Like in most plants, this particular species contains high amount of secondary metabolites, thus rendering the isolation of high quality and quantity RNA extract for molecular applications rather challenging. An effective, high-yield and high-quality RNA isolation protocol for this economically important plant species was devised here based on the cetyltrimethylammonium bromide (CTAB) extraction method, with an additional genomic DNA (gDNA) removal step. DNA and other contaminants that may affect downstream applications were effectively removed. Our results exhibited that RNA samples isolated from the leaves and stems of Stevia rebaudiana using this improvised method are high in integrity and quality with RNA integrity number (RIN) of more than 8 and low in contaminants.

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“…(2011) were also utilised in several studies to isolate RNA from stevia species (Madhav et al . 2013; Modi et al . 2014; Michalec-Warzecha et al .…”

Section: Discussionmentioning

confidence: 99%

“…Several studies had been reported on the isolation of RNA from this plant using conventional methods based on acid guanidinium thiocyanate-phenol-chloroform (Michalec-Warzecha et al . 2016) and phenol/SDS based IHBT (Modi et al . 2014); as well as commercial kits (J Chen et al .…”

Section: Introductionmentioning

confidence: 99%