Analysis of Differentially Expressed Genes and Signaling Pathways Related to Intramuscular Fat Deposition in Skeletal Muscle of Sex-Linked Dwarf Chickens

Ye, Yaqiong; Lin, Senjie; Mu, Heping; Tang, Xiaohong; Ou, Yangdan; Chen, Jian; Ma, Yue; Li, Yugu

doi:10.1155/2014/724274

Cited by 30 publications

(31 citation statements)

References 41 publications

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“…In this study, XLOC_064871 can trans-regulate TRIB3, indicating that XLOC_064871 might also play a vital role in adipocyte differentiation and fatty acid metabolism in pig. NR1D2 was highly expressed in skeletal muscle of Bantam chicken with high intramuscular fat content, and can induce the expression of PPARs, CEBPα and FABP4 genes, promoting adipogenic differentiation and affecting intramuscular fat deposition [74]. In this study, the expression of NR1D2 in intramuscular adipose tissue of LW pig was high, which was consistent with previous study.…”

Section: Pigssupporting

confidence: 91%

Genome-Wide Analysis of mRNAs and lncRNAs of Intramuscular Fat Related to Lipid Metabolism in Two Pig Breeds

Huang

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Long non-coding RNAs (lncRNAs) can regulate adipogenesis and lipid accumulation. Intramuscular fat deposition appears to vary in different pig breeds, and the regulation mechanism has not yet been fully elucidated at molecular level. Moreover, little is known about the function and profile of lncRNAs in intramuscular fat deposition and metabolism in pig. The aim of this study was thus to explore the regulatory functions of lncRNAs in intramuscular fat deposition. Methods: In this study, Laiwu (LW) pig and Large White (LY) pig with significant difference in fat deposition were selected for use. RNA-seq technology and bioinformatics methods were used to comparatively analyze the gene expression profiles of intramuscular fat between LW and LY pigs to identify key mRNAs and lncRNAs associated with lipid metabolism and adipogenesis. Real-time fluorescence-based quantitative PCR was applied to verify the expression level of the differentially expressed mRNAs and lncRNAs. Results: A total of 513 mRNAs and 55 lncRNAs were differentially expressed between two pig breeds. By co-expression network construction as well as cis- and trans-regulated target gene analysis, 31 key lncRNAs were identified. Gene Ontology and KEGG pathway analyses revealed that differentially expressed genes and lncRNAs were mainly involved in the biological processes and pathways related to adipogenesis and lipid metabolism. Conclusion: XLOC_046142, XLOC_004398 and XLOC_015408 may target MAPKAPK2, NR1D2 and AKR1C4, respectively, and play critical regulatory roles in intramuscular adipogenesis and lipid accumulation in pig. XLOC_064871 and XLOC_011001 may play a role in lipid metabolism-related disease via regulating TRIB3 and BRCA1. This study provides a valuable resource for lncRNA study and improves our understanding of the biological roles of lipid metabolism- related genes and molecular mechanism of intramuscular fat metabolism and deposition.

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Section: Pigssupporting

confidence: 91%

Genome-Wide Analysis of mRNAs and lncRNAs of Intramuscular Fat Related to Lipid Metabolism in Two Pig Breeds

Huang

Zhang

et al. 2018

Cell Physiol Biochem

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“…Next-generation sequencing has facilitated the research on the regulation of IMF deposition in chicken [ 24 ], cattle [ 25 ], and pigs [ 26 ]. Despite the characterization of the complete transcriptome of the goat mammary gland [ 27 ] and skin [ 28 ], to date, no information is available on the transcriptome for goat muscle development.…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages

Lin¹,

Zhu²,

Wang³

et al. 2017

PLoS ONE

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Intramuscular fat (IMF) content and fatty acid composition of longissimus dorsi muscle (LM) change with growth, which partially determines the flavor and nutritional value of goat (Capra hircus) meat. However, unlike cattle, little information is available on the transcriptome-wide changes during different postnatal stages in small ruminants, especially goats. In this study, the sequencing reads of goat LM tissues collected from kid, youth, and adult period were mapped to the goat genome. Results showed that out of total 24 689 Unigenes, 20 435 Unigenes were annotated. Based on expected number of fragments per kilobase of transcript sequence per million base pairs sequenced (FPKM), 111 annotated differentially expressed genes (DEGs) were identified among different postnatal stages, which were subsequently assigned to 16 possible expression patterns by series-cluster analysis. Functional classification by Gene Ontology (GO) analysis was used for selecting the genes showing highest expression related to lipid metabolism. Finally, we identified the node genes for lipid metabolism regulation using co-expression analysis. In conclusion, these data may uncover candidate genes having functional roles in regulation of goat muscle development and lipid metabolism during the various growth stages in goats.

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“…The results indicate that the selection against abdominal fat resulted in a higher IMFC. The IMFC is an indicator of meat quality in animals, which is related with sensory satisfaction, meat flavour, tenderness and juiciness (Eikelenboom et al 1996;Fernandez et al 1999;Gerbens et al 2001;Suzuki et al 2005;Ye et al 2014).…”

Section: Selection Against Abdominal Fat Resulted In Higher Imfcmentioning

confidence: 99%

“…; Ye et al . ). Together with the result that there was a significant negative genetic correlation between IMFC and AFC, and the differences in IMFC grew from G8 on, these results indicated that they were attending the selection for lean line.…”

Section: Discussionmentioning

confidence: 97%

Selection against abdominal fat percentage may increase intramuscular fat content in broilers

Leng

Zhang

Dong

et al. 2016

J Animal Breeding Genetics

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Excessive abdominal fat content (AFC) has negative impacts on feed efficiency and carcass quality. Unlike AFC, intramuscular fat content (IMFC) could be a favourable trait, which has a positive impact on meat quality. To meet consumers' needs, a long-term goal of broiler breeders is to decrease AFC and improve the IMFC simultaneously. The current study was designed to investigate the relationship between AFC and IMFC and to compare IMFC, including the pectoral major muscle fat content (PIMFC) and intramuscular fat content of leg muscle (LIMFC), between two broiler lines divergently selected for abdominal fat percentage over 17 generations. The results showed that there was a significant difference in PIMFC and LIMFC between the two lines in all five generation populations used. The birds in the lean line had significantly lower AFC but higher PIMFC and LIMFC than the birds in the fat line. We also detected differences in the liver fat content (LFC) between the two lines and the results showed that birds in the fat line had significant higher LFC than birds in the lean line. Our results indicated that a desirable broiler line with higher IMFC but lower AFC could be obtained by genetic selection.

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Analysis of Differentially Expressed Genes and Signaling Pathways Related to Intramuscular Fat Deposition in Skeletal Muscle of Sex-Linked Dwarf Chickens

Cited by 30 publications

References 41 publications

Genome-Wide Analysis of mRNAs and lncRNAs of Intramuscular Fat Related to Lipid Metabolism in Two Pig Breeds

Genome-Wide Analysis of mRNAs and lncRNAs of Intramuscular Fat Related to Lipid Metabolism in Two Pig Breeds

Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages

Selection against abdominal fat percentage may increase intramuscular fat content in broilers

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