Analysis of Differentially Regulated Proteins in TM4 Cells Treated with Bisphenol A

Lee, Do-Youn; Lee, Sang Soo; Joo, Won A.; Lee, Eun Ju; Kim, Chan Wha

doi:10.1271/bbb.68.1201

Cited by 17 publications

(11 citation statements)

References 28 publications

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“…Cell damage was measured using LDH efflux assays and observed only at concentrations (>150 µM) well above those used in these studies for examining P-gp activity. Similar results were obtained in TM4 cells when after a 16 hr exposure to 200 μM of BPA, cell viability was dramatically decreased (38).…”

Section: Discussionsupporting

confidence: 85%

Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line

Audus

2005

Placenta

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. (2005) Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line. Placenta, 26 Suppl.A, S96-S103. PMID: 15837075. Publisher's official version: http://dx.doi.org/10.1016/j.placenta. Keywords:Bisphenol A (BPA), P-glycoprotein (P-gp), BeWo cells, Drug efflux and 17β-estradiol (E2) Abstract:Bisphenol A (BPA) is a monomer of polycarbonate plastics that has estrogenic activities and has been shown to be a substrate for multidrug resistant efflux mechanisms, specifically, P-glycoprotein. Since the natural hormone estrogen reverses multidrug resistance in some cell types, we hypothesized that BPA might have a similar activity in trophoblasts. We have used BeWo cells as an in vitro model for human trophoblasts and calcein AM as a substrate for drug efflux mechanism to characterize BPA interactions with placental P-glycoprotein. We found that chronic exposure of BeWo cells to BPA did not alter intracellular calcein accumulation in a fashion that would be reflective of changes in Pglycoprotein expression. However, BeWo cells acutely exposed to BPA pre-treatment were observed to have a significantly decreased calcein accumulation suggesting a direct stimulatory effect on P-gp. Addition of cyclosporin A, a P-glycoprotein inhibitor and substrate, completely reversed BPA's effects on calcein accumulation and resulted in a net increase, relative to controls, in calcein accumulation by the BeWo cells. BPA was found not stimulate P-gp ATPase. Therefore, our results suggested that BPA stimulated drug efflux by BeWo cells probably by direct effects on P-glycoprotein.

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Section: Discussionsupporting

confidence: 85%

Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line

Audus

2005

Placenta

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“…The recent development of proteomic approaches has provided researchers with useful tools for identifying protein markers of toxic chemical exposure. Proteomics investigations have been conducted in several cell systems to investigate potential biomarkers of BPA exposure, most importantly, in Sertoli cells [ 12 , 76 ], thyroid [ 77 ], serum [ 8 ], mammary gland [ 14 , 78 ], zebrafish brain [ 79 ], rat hippocampus [ 13 ], mouse prefrontal cortex [ 28 ], colon cancer cells (SW480) [ 80 ], and Leydig cells [ 81 ].…”

Section: Resultsmentioning

confidence: 99%

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Ryu

Rahman

Pang

2017

IJMS

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Bisphenol-A (BPA) is a ubiquitous endocrine-disrupting chemical. Recently, many issues have arisen surrounding the disease pathogenesis of BPA. Therefore, several studies have been conducted to investigate the proteomic biomarkers of BPA that are associated with disease processes. However, studies on identifying highly sensitive biological cell model systems in determining BPA health risk are lacking. Here, we determined suitable cell model systems and potential biomarkers for predicting BPA-mediated disease using the bioinformatics tool Pathway Studio. We compiled known BPA-mediated diseases in humans, which were categorized into five major types. Subsequently, we investigated the differentially expressed proteins following BPA exposure in several cell types, and analyzed the efficacy of altered proteins to investigate their associations with BPA-mediated diseases. Our results demonstrated that colon cancer cells (SW480), mammary gland, and Sertoli cells were highly sensitive biological model systems, because of the efficacy of predicting the majority of BPA-mediated diseases. We selected glucose-6-phosphate dehydrogenase (G6PD), cytochrome b-c1 complex subunit 1 (UQCRC1), and voltage-dependent anion-selective channel protein 2 (VDAC2) as highly sensitive biomarkers to predict BPA-mediated diseases. Furthermore, we summarized proteomic studies in spermatozoa following BPA exposure, which have recently been considered as another suitable cell type for predicting BPA-mediated diseases.

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“…As previously discussed, some proteins/subsets of proteins are found at a high frequency in this area of toxicoproteomics of industrial products, illustrating their central role in response to toxic injury. HSP proteins (mostly hsp70 and hsp27) are found in 14 of 30 papers , . Mitochondrial proteins, most often matricial enzymes, are found in 14 of 30 papers .…”

Section: Proteomics For Studying Biological Responses To Toxicantsmentioning

confidence: 99%

“…Furthermore, as the toxicoproteomic studies on industrial chemicals are carried out on a limited range of chemicals, it is possible (and tempting) to compare various studies carried out on the same chemical in different papers (e.g. vs. on benzopyrene, vs. on bisphenol A, vs. on dibutylphthalate, and vs. on dioxin). These comparisons show that proteomic studies carried out on the same chemical by different groups usually share a moderate overlap in the lists of modulated proteins.…”

Section: Proteomics For Studying Biological Responses To Toxicantsmentioning

confidence: 99%

Proteomics in mechanistic toxicology: History, concepts, achievements, caveats, and potential

Rabilloud

Lescuyer

2014

Proteomics

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Toxicoproteomics can be defined as the application of proteomic approaches to the understanding of toxicology problems, and this review deals with the various types of applications that have been described in the literature. Toxicoproteomics has been applied to very different classes of toxicants, from drugs and natural products to metals, or from industrial chemicals to nanoparticles and nanofibers. It has also been applied to address questions at different levels, from the search of the primary molecular targets of toxicants to the deciphering of the molecular responses of cells and tissues to toxicants. Although restricted to mammalian cells and tissues, this paper reviews these two levels of investigation and the different application areas of toxicoproteomics, leading to the discussion of the advantages and drawbacks of the most popular proteomic platforms. Some of the pending questions in toxicoproteomics are also critically addressed, such as the specificity, validation, and result hierarchization issues. The question of shared mechanisms, which are encountered in many toxicoproteomic papers dealing with different toxicants, is also discussed. Finally, the future of toxicoproteomics is briefly outlined.

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Analysis of Differentially Regulated Proteins in TM4 Cells Treated with Bisphenol A

Cited by 17 publications

References 28 publications

Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line

Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Proteomics in mechanistic toxicology: History, concepts, achievements, caveats, and potential

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