Analysis of diurnal and vertical microbial diversity of a hypersaline microbial mat

Villanueva, Laura; Navarrete, Antoni; Urmeneta, Jordi; White, David C.; Guerrero, Ricardo

doi:10.1007/s00203-007-0229-6

Cited by 26 publications

(13 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our observations do show some difference from the past studies. A recent publication using phospholipid fatty acid analysis and denaturing gradient gel electrophoresis to characterize depth‐related population structure of another hypersaline mat (Camargue, France) (Caumette et al , 1994) suggested that microniches were more important than time of day in determining community structure (Villanueva et al , 2007). This contrasts with our data showing that, although depth stratification was clearly an important factor, time of day explained the majority of the variation in T‐RFLP profiles.…”

Section: Discussionmentioning

confidence: 99%

“…Morphology, a principle tool used in those past identifications, is well known to conceal diversity. Molecular analyses are being increasingly used to characterize community structure in mats (Risatti et al, 1994;Ward et al, 1998;Mouné et al, 2003;Casillas-Martinez et al, 2005;Fourçans et al, 2006;Desnues et al, 2007;Villanueva et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spatial and temporal variability in a stratified hypersaline microbial mat community

Dillon

Miller

Bebout

et al. 2009

FEMS Microbiology Ecology

View full text Add to dashboard Cite

Hypersaline microbial mat communities have recently been shown to be more diverse than once thought. The variability in community composition of hypersaline mats, both in terms of spatial and temporal dimensions, is still poorly understood. Because this information is essential to understanding the complex biotic and abiotic interactions within these communities, terminal restriction fragment analysis and 16S rRNA gene sequencing were used to characterize the near-surface community of a hypersaline microbial mat in Guerrero Negro, Mexico. Core samples were analyzed to assay community variability over large regional scales (centimeter to kilometer) and to track depth-related changes in population distribution at 250-microm intervals over a diel period. Significant changes in total species diversity were observed at increasing distances across the mat surface; however, key species (e.g. Microcoleus sp.) were identified throughout the mat. The vertical position and abundance of >50% of the 60 peaks detected varied dramatically over a diel cycle, including Beggiatoa sp., cyanobacteria, Chloroflexus sp., Halochromatium sp., Bacteroidetes sp. and several as-yet-identified bacteria. Many of these migrations correlated strongly with diel changes in redox conditions within the mat, contributing to strong day-night community structure differences.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatial and temporal variability in a stratified hypersaline microbial mat community

Dillon

Miller

Bebout

et al. 2009

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…2). It is assumed, based on previous work (Villanueva et al, 2007) that depth-related differences in structure are more important than diel variations in controlling the distribution of microorganisms.…”

Section: Microbiology and Palynologymentioning

confidence: 99%

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Słowakiewicz

Whitaker

Thomas

et al. 2016

Organic Geochemistry

View full text Add to dashboard Cite

General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractUnderstanding early organic matter alteration and preservation in marine carbonate-evaporite systems could improve understanding of carbon cycling and hydrocarbon source rock prediction in such environments. It is possible that organic-rich microbial mats are important contributors to preserved hydrocarbons, and to explore this we examined changes in lipid composition in such a mat from a mesohaline intertidal lagoon, eastern Qatar. The mat reaches > 5 cm thickness over a carbonate mud substrate, rich in seagrass, gastropods and other small bioclasts. Clear lamination, with distinct downward colour changes from green to pink to brown, reflects different microbial mat communities. The layers contain spheroids of probable dolomite, the precipitation of which was plausibly bacterially-mediated. Lipids [n-alkanes, fatty acids (FAs), hopanoids, isoprenoid hydrocarbons, dialkyl glycerol diethers (DAGEs), and isoprenoid (0 to 4) and branched (Ia to IIIa) GDGTs] reflect the diverse mat-building phototrophic, heterotrophic and chemoorganotrophic microorganisms, as well as some likely allochthonous material (i.e. steroids, n-alkanols, high molecular weight nalkanes). The lipids clearly document the change in microbial community, with phytene being the predominant hydrocarbon in the phototrophic surface layer. Oxygen and pH drop significantly 0.2 cm below the mat surface, coincident with the predominance of Deltaproteobacteria and increased concentrations of archaeal and bacterial glycerol dialkyl glycerol tetraether lipids andC 15 /C 16 and C 16 /C 17 dialkyl glycerol ether lipids in the deeper layers. Archaeol, likely of methanogen origin, is most abundant in the deepest layer. Allochthonous inputs occur throughout the mat, including abundant steroids, especially dinosterol and dinostanol, that are possibly related to periodic algal (dinoflagellate) blooms in the Arabian Gulf. Both n-alkanes and n-alkanols appear to be derived from seagrass. Organic matter contents decrease in the deepest mat; this suggests an overall low preservation potential of OM in intertidal mesohaline-hypersaline mats of the Arabian Gulf, which in turn suggests that these are not the major source of hydrocarbons in microbially-dominated carbonateevaporite systems.2

show abstract

“…In turn, 18:4x3 pointed to the presence of diatoms. In another study, Villanueva et al (2007) observed the daily migrations of micro-organisms in an intertidal hypersaline microbial mat in France. They reported that the total PLFAs that indicated the maximum viable biomass were the highest at the top of the mat in the morning hours, while in the afternoon the highest volume was found underneath the uppermost layers.…”

Section: Microbial Diversity In Biomatsmentioning

confidence: 99%

Microbial diversity in waters, sediments and microbial mats evaluated using fatty acid-based methods

Mrozik

Nowak

Piotrowska‐Seget

2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

The review summarises recent advances towards a greater comprehensive assessment of microbial diversity in aquatic environments using the fatty acid methyl esters and phospholipid fatty acids approaches. These methods are commonly used in microbial ecology because they do not require the culturing of micro-organisms, are quantitative and reproducible and provide valuable information regarding the structure of entire microbial communities. Because some fatty acids are associated with taxonomic and functional groups of micro-organisms, they allow particular groups of micro-organisms to be distinguished. The integration of fatty acid-based methods with stable isotopes, RNA and DNA analyses enhances our knowledge of the role of micro-organisms in global nutrient cycles, functional activity and phylogenetic lineages within microbial communities. Additionally, the analysis of fatty acid profiles enables the shifts in the microbial diversity in pristine and contaminated environments to be monitored. The main objective of this review is to present the use of lipid-based approaches for the characterisation of microbial communities in water columns, sediments and biomats.

show abstract

Analysis of diurnal and vertical microbial diversity of a hypersaline microbial mat

Cited by 26 publications

References 47 publications

Spatial and temporal variability in a stratified hypersaline microbial mat community

Spatial and temporal variability in a stratified hypersaline microbial mat community

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Microbial diversity in waters, sediments and microbial mats evaluated using fatty acid-based methods

Contact Info

Product

Resources

About