Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers

Luo, Cong; He, Xinhua; Hu, Chaochao; Ou, Songbang; Gao, Mei-ping

doi:10.1016/j.bse.2010.11.004

Cited by 125 publications

(89 citation statements)

References 15 publications

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“…The SCoT primers were screened according to the methods described by Collard and Mackill (2009) and Luo et al (2010). In prescreening assays with 4 orchardgrass accessions and 80 SCoT primers, clear reproducible polymorphic amplification products were generated by 24 primers, which were selected for genetic relationship analysis of orchardgrass in this study.…”

Section: Scot Polymorphism and Genetic Diversitymentioning

confidence: 99%

“…SCoT markers are designed from the short conserved region flanking the ATG start codon of all plant genes (Collard and Mackill, 2009). Previously, more than 80 primers were released for SCoT analysis (Collard and Mackill, 2009;Luo et al, 2010). Compared with other molecular markers, SCoT markers have emerged as an alternative system with several advantages: lower cost than AFLP, higher reproducibility than RAPD, and easier development of species-specific primers than SSR (Guo et al, 2012).…”

Section: Scot Polymorphism Of Orchardgrassmentioning

confidence: 99%

“…Compared with other molecular markers, SCoT markers have emerged as an alternative system with several advantages: lower cost than AFLP, higher reproducibility than RAPD, and easier development of species-specific primers than SSR (Guo et al, 2012). Luo et al (2010) showed that amplification products generated from the SCoT marker technique were associated with functional genes and their corresponding traits. Xiong et al (2011) showed that SCoT markers could be utilized to identify DNA polymorphisms and fingerprint cultivars in domesticated peanut.…”

Section: Scot Polymorphism Of Orchardgrassmentioning

confidence: 99%

“…These types of molecular techniques included random amplified polymorphic DNA (RAPD) (Kölliker et al, 1999;Tuna et al, 2004), amplified fragment length polymorphism (AFLP) (Reeves et al, 1998;Peng et al, 2008), inter-simple sequence repeat (ISSR) (Zeng et al, 2006), sequence-related amplified polymorphism (Zeng et al, 2008;Xie et al, 2011), simple sequence repeats (SSRs) (Hirata et al, 2011;Xie et al, 2011Xie et al, , 2012, and expressed sequence tag-SSRs (Xie et al, 2010;Bushman et al, 2011). These marker systems are useful for biodiversity analyses, phylogenetic studies, germplasm management, cultivar identification, and other applications (Semagn et al, 2006;Luo et al, 2010). However, the investigation of genetic diversity by molecular markers for breeding purposes should be based on functionally characterized genes or targeted genes (Andersen and Lübber-stedt, 2003) because these markers may reflect functional polymorphisms indirectly.…”

Section: Introductionmentioning

confidence: 99%

“…Of these molecular markers, start codon-targeted (SCoT) polymorphism is a novel, simple, highly polymorphic, and reliable gene-targeted marker technique that is based on a single primer amplification reaction, which was designed using a single primer according to the conservation of the ATG translation start site and flanking sequences in plant genes (Collard and Mackill, 2009;Luo et al, 2010;Guo et al, 2012). In addition, the SCoT technique is convenient for marker-assisted breeding because it effectively produces markers that are linked to traits.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of diversity and relationships among orchardgrass (Dactylis glomerata L.) accessions using start codon-targeted markers

Lf¹,

X²,

Xq³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Orchardgrass, or cocksfoot, is an important perennial forage grass worldwide. The comprehensive understanding of orchardgrass accessions will benefit germplasm collection and breeding progress, and it will enhance efforts to improve forage yield and quality. Therefore, 24 novel, simple, polymorphic, and reliable start codon-targeted (SCoT) markers were used to analyze the diversity and genetic relationships among 95 orchardgrass accessions. In total, 273 polymorphic bands were detected with an average of 11.4 bands per primer. The average polymorphic rate for the species was 83.4%, suggesting a high discriminating ability of the SCoT technique for orchardgrass. The molecular variance analysis revealed that 69.13 and 30.87% of variation resided within and among groups, respectively, demonstrating that the orchardgrass germplasms had a higher level of Analysis of Dactylis glomerata using SCoT markers genetic diversity within groups than among geographical regions and distributions. The distinct geographical divergence of orchardgrass was revealed between North America and Oceania. The unweighted pairgroup method with arithmetic mean dendrogram revealed a separation of 7 main clusters between 95 accessions according to the geographical origin. Furthermore, each cluster was divided into subgroups mainly according to the origin of its state. The genetic divergence of orchardgrass might be influenced by the ecogeographical conditions, climatic types, breeding systems and gene flow with variations in cultures, bird migration, and breeder selection. These results could facilitate orchardgrass germplasm collection, management, and breeding worldwide.

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Section: Scot Polymorphism and Genetic Diversitymentioning

confidence: 99%

Section: Scot Polymorphism Of Orchardgrassmentioning

confidence: 99%

Section: Scot Polymorphism Of Orchardgrassmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of diversity and relationships among orchardgrass (Dactylis glomerata L.) accessions using start codon-targeted markers

Lf¹,

X²,

Xq³

et al. 2014

Genet. Mol. Res.

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Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison

Noppradit,

Klinnawee,

Leeratiwong

et al. 2024

Phytochemical Analysis

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IntroductionCentella is an important genus in the Apiaceae family. It includes Centella asiatica, which has significant edible and medicinal values. However, this species is easily confused due to its similar morphological traits to Hydrocotyle umbellata, hindering its utilization in the consumer and pharmacological industries.ObjectiveThe study aims to differentiate these two closely related plant species using reliable methods of confirming the authenticity of natural herbal medicines.MethodsOur work mainly focuses on the basic morphological characteristics, chemical markers, genetic fingerprints, and their biological responses.ResultsThe plants can be clearly differentiated using their leaf shapes, stipules, petioles, inflorescences, and fruit structures. Although the phytochemical compositions of the C. asiatica extract were similar to that of H. umbellata which included flavonoids, tannins, and saponins important to the plant's ability to reduce inflammation and promote healing of wounds, the H. umbellata extract showed significantly higher toxicity than that of C. asiatica. High‐performance liquid chromatography analysis was used to identify chemical fingerprints. The result revealed that C. asiatica had major triterpene glycoside constituents including asiaticoside, asiatic acid, madecassoside, and madecassic acid, which have a wide range of medicinal values. In contrast, triterpenoid saponins were not identified in H. umbellata. Furthermore, using SCoT1–6 primers was possible to effectively and sufficiently created a dendrogram which successfully identified the closeness of the plants and confirmed the differences between the two plant species.ConclusionTherefore, differentiation can be achieved through the combination of morphometrics, molecular bioactivity, and chemical analysis.

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Genetic Diversity Analysis of Mango

Razak

Luo

2021

Compendium of Plant Genomes

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Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers

Cited by 125 publications

References 15 publications

Analysis of diversity and relationships among orchardgrass (Dactylis glomerata L.) accessions using start codon-targeted markers

Analysis of diversity and relationships among orchardgrass (Dactylis glomerata L.) accessions using start codon-targeted markers

Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison

Genetic Diversity Analysis of Mango

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