Analysis of diversity of actinomycetes from arid and saline soils at Rajasthan, India

Binayke, Akshay; Ghorbel, Sofiane; Hmidet, Noomen; Raut, Amol D.; Gunjal, Aparna; Uzgare, Ashish; Patil, N. S.; Waghmode, M.; Nawani, Neelu

doi:10.1007/s42398-018-0003-5

Cited by 12 publications

(6 citation statements)

References 27 publications

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“…Approximately 22,500 biologically active substances are obtained from microorganisms, 45% of which are represented by Actinomycetes and 70% of which are Streptomyces metabolites; however, information on substances isolated from microorganisms inhabiting saline environments is scarce [1,2,3]. The search for these microorganisms has been mainly associated with the production of antibiotics and antitumor substances [4].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Comparison Using Streptomyces spp as a Factory of Secondary Metabolites

Clavo¹,

Pereira²,

Quiñones³

et al. 2022

Preprint

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Understanding extremophiles and their usefulness in biotechnology involves studying their habitat, physiology and biochemical adaptations , as well as their ability to produce biocatalysts, in environments that are still poorly explored. In northwestern Peru, which saline lagoons of marine origin Pacific Ocean, the other site from the coast of Brazil of the Atlantic Ocean. Both environments are considered extreme. The objective of the present work was to compare two different strains isolated from these extreme environments at the metabolic level using molecular network methodology through the Global Natural Products Molecular Social Network (GNPS). In our study, the MS/MS spectra from the network were compared with GNPS spectral libraries, where the metabolites were annotated. Differences were observed in the molecular network presented in the two strains of Streptomyces spp. coming from these two different environments. Within the annotated compounds from marine bacteria, the metabolites characterized for Streptomyces sp. B-81 from Peruvian marshes were lobophorins A (1) and H (2), as well as divergolides A (3), B (4) and C (5). Streptomyces sp. 796.1 produced different compounds, such as glucopiericidin A (6) and dehydro-piericidin A1a (7). The search for new metabolites in underexplored environments may therefore reveal new metabolites with potential application in different areas of biotechnology.

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Section: Introductionmentioning

confidence: 99%

Metabolomic Comparison Using Streptomyces spp as a Factory of Secondary Metabolites

Clavo¹,

Pereira²,

Quiñones³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Metabolomic comparison usingStreptomycesspp. as a factory of secondary metabolites

Clavo

Pereira

Quiñones³

et al. 2022

Preprint

View full text Add to dashboard Cite

Understanding extremophiles and their usefulness in biotechnology involves studying their habitat, physiology and biochemical adaptations, as well as their ability to produce biocatalysts, in environments that are still poorly explored. In northwestern Peru, which has saline lagoons of marine origin Pacific Ocean, the other site is from the coast of Brazil of the Atlantic Ocean. Both environments are considered extreme. The objective of the present work was to compare two different strains isolated from these extreme environments at the metabolic level using molecular network methodology through the Global Natural Products Molecular Social Network (GNPS). In our study, the MS/MS spectra from the network were compared with GNPS spectral libraries, where the metabolites were annotated. Differences were observed in the molecular network presented in the two strains of Streptomyces spp. coming from these two different environments. Within the annotated compounds from marine bacteria, the metabolites characterized for Streptomyces sp. B-81 from Peruvian marshes were lobophorins A (1) and H (2), as well as divergolides A (3), B (4) and C (5). Streptomyces sp. 796.1 produced different compounds, such as glucopiericidin A (6) and dehydro-piericidin A1a (7). The search for new metabolites in underexplored environments may therefore reveal new metabolites with potential application in different areas of biotechnology.

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“…Micromonospora , Streptomyces , Salinispora , and Dietzia have been isolated from the coastal zone in Chile [ 6 ]. Additionally, halophilic and halotolerant strains of Actinobacteria show heterogeneous physiological characteristics for different genera because these bacteria can synthesize secondary metabolites to cope with the high salinity and extreme temperature conditions of their environments [ 7 – 9 ]. These extreme conditions favor the development of metabolic competitiveness to the production enzyme, which can bacterial population adapt to high salinity [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru

et al. 2021

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Extreme environments Morrope and Bayovar Salt lagoons, several ecosystems and microhabitats remain unexplored, and little is known about the diversity of Actinobacteria. We suggest that the endemic bacteria present in this extreme environment is a source of active molecules with anticancer, antimicrobial, and antiparasitic properties. Using phenotypic and genotypic characterization techniques, including 16S rRNA sequencing, we identified these bacteria as members of the genera Streptomyces, Pseudonocardia, Staphylococcus, Bacillus, and Pseudomonas. Actinobacteria strains were found predominantly. Phylogenetic analysis revealed 13 Actinobacteria clusters of Streptomyces, the main genus. Three Streptomycetes, strains MW562814, MW562805, and MW562807 showed antiproliferative activities against three tumor cell lines: U251 glioma, MCF7 breast, and NCI-H460 lung (non-small cell type); and antibacterial activity against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, and the multidrug resistant Acinetobacter baumannii AC-972. The antiproliferative activities (measured as total growth inhibition [TGI]) of Streptomyces sp. MW562807 were 0.57 μg/mL, for 0.61 μg/mL, and 0.80 μg/mL for glioma, lung non-small cell type, and breast cancer cell lines, respectively; the methanolic fraction of the crude extract showed a better antiproliferative activity and could inhibit the growth of (U251 (TGI = 38.3 μg/mL), OVCAR-03 (TGI = 62.1 μg/mL), and K562 (TGI = 81.5 μg/mL)) of nine tumor cells types and one nontumor cell type. Extreme enviroments, such as the Morrope and Bayovar Salt saloons are promising sources of new bacteria, whose compounds may be useful for treating various infectious diseases or even some types of cancer.

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Analysis of diversity of actinomycetes from arid and saline soils at Rajasthan, India

Cited by 12 publications

References 27 publications

Metabolomic Comparison Using Streptomyces spp as a Factory of Secondary Metabolites

Metabolomic Comparison Using Streptomyces spp as a Factory of Secondary Metabolites

Metabolomic comparison usingStreptomycesspp. as a factory of secondary metabolites

Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru

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