Analysis of DNA adducts using high-performance separation techniques coupled to electrospray ionization mass spectrometry

Analysis of mono-phosphate nucleotides as a potential method for quantification of DNA using high performance liquid chromatography–inductively coupled plasma-mass spectrometry

“…Elemental analysis is preferred over other techniques as it offers lower detection limits, a larger dynamic range, a response that is to a first approximation independent of molecular form, and potentially more accurate quantification at low concentrations by isotope dilution analysis. Both ICP-MS and ICP-OES have been utilised for the quantification of nucleotides, DNA, oligonucleotides and DNA adducts [3][4][5][6][7][8][9][10][11][12][13][14][15]. The quantification of oligonucleotides has been achieved by two different methods by Yang et al [4] and Donald et al [7], with the latter group digesting the oligonucleotides to mono-nucleosides and quantifying by isotope dilution electrospray mass spectrometry (ES-ID-MS), and comparing that value to the total 31 P signal obtained from ICP-OES and the gravimetric value.…”

Investigation of the regio- and stereo-selectivity of deoxyguanosine linkage to deuterated 2-hydroxyestradiol by using liquid chromatography/ESI-ion trap mass spectrometry

“…Because of its sensitivity and specificity, mass spectrometry appears to be a powerful tool for exploring this field of investigation [20 -22]. Moreover, the possibility of on-line coupling with separation techniques, such as HPLC or CE, enhances the detection capabilities of this method [23][24][25]. Among different soft desorption-ionization techniques (e.g., MALDI, FAB, TSP), electrospray ionization is now commonly used for DNA adduct analysis, because of the very soft desolvation conditions which avoid fragile bond cleavages.…”

Use of DNA adducts to identify human health risk from exposure to hazardous environmental pollutants: The increasing role of mass spectrometry in assessing biologically effective doses of genotoxic carcinogens

“…Singh and Farmer [34], with particular reference to electrospray ionisation (ESI)-liquid chromatography (LC)-MS, Esmans et al [35] Andrews et al [36], Doerge et al [37], Koc and Swenberg [38] and Watson et al [39]. The approaches most usually employ collision induced dissociation (CID) tandem MS (MS/MS) with selected reaction monitoring (SRM) for the determination of:…”