Analysis of DNA Damage-Binding Proteins (DDBs) in Arabidopsis thalian a and their Protection of the Plant from UV Radiation

Gawwad, Mohamed Ragab Abdel; Kadunic, Aida; Adilovic, Muhamed; Kaljanac, Aida Hamzi; Maric, Aida

doi:10.2174/1570164614666161205143327

Cited by 6 publications

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“…We investigated expression patterns during pre-storage cold acclimation (PsCA) treatment and cold storage for DDI1, DDR1 and DDB1, which are all involved in DNA repair responses (Al Khateeb and Schroeder, 2009;Fujimori et al, 2014;Maric et al, 2017). In fruit exposed to cold stress from the very beginning (the control), CsDDI1 expression remained almost unchanged until 6 d, while that of the PsCA-treated cucumber significantly increased following 3 d of cold acclimation and kept increasing even after the fruit were placed in chilling stress condition ( Figure 1A).…”

Section: Expression Of Csddi1 Ddr1 and Ddb1 Genes In Response To Comentioning

confidence: 99%

“…Plants have evolved the ability to cope with various environmental stresses to ensure survival and proliferation, including solar UV and ionizing radiation, chemical mutagens, heavy metals, droughts, heat, pathogenic attacks, and chilling. Although different environmental stresses may cause different disorders or symptoms in plants, they can all cause cellular DNA damage (Durrant et al, 2007;Gichner et al, 2008;Ciccia and Elledge, 2010;Achary et al, 2012;Cvjetko et al, 2014;Roy, 2014;Daghino et al, 2016;Ding et al, 2016;Maric et al, 2017;Shim et al, 2018). DNA damage may result in changes to both the chemical and physical structures of DNA, which can seriously threaten the survival and the faithful transmission of genetic information in plants (Zhang et al, 2015a;Ding et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The organisms may initiate responses to defend itself against DNA damage. DNA damage-inducible (DDI) proteins are usually related to plant defense responses and play important roles in DNA repair pathways (Maric et al, 2017). Arabidopsis DNA damage-inducible protein 1 (AtDDI1) participates in plant defense responses against abiotic stresses such as drought and salt by regulating the expression of defense genes (Ding et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

Wang

Zhu

2020

Front. Plant Sci.

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Chilling stress can cause cellular DNA damage, affecting the faithful transmission of genetic information. Cold acclimation enhances chilling tolerance, but it is not clear that the process of cold adaption involves DNA damage responses, as cold acclimation does not form real chilling stress. Here we showed with cucumber fruit that pre-storage cold acclimation (PsCA) reduces chilling injury and upregulates DNA damage inducible protein1 (CsDDI1), suggesting that the chilling tolerance induced by cold acclimation involves CsDDI1 transcription. Application of nitric oxide (NO), abscisic acid (ABA) or H 2 O 2 biosynthesis inhibitor before PsCA treatment downregulates CsDDI1 and aggravates chilling injury, while H 2 O 2 generation inhibition plus exogenous NO or ABA application before PsCA treatment restores chilling tolerance, but does not restore CsDDI1 expression, suggesting H 2 O 2 plays a crucial role in triggering cold adaption. CsDDI1 overexpression Arabidopsis lines show faster growth, stronger chilling tolerance, lower reactive oxygen species levels, enhanced catalase and superoxide dismutase activities and higher expression of nine other Arabidopsis defense genes under chilling stress, suggesting CsDDI1 strengthens defenses against chilling stress by enhancing antioxidant defense system. Taken together, CsDDI1 positively regulates chilling tolerance induced by cold acclimation in cucumber. In addition, H 2 O 2 is involved in initiation of cold acclimation. While CsDDI1 upregulation requires H 2 O 2 as a key signaling molecule, the upregulation of CsDDI1 activates an antioxidant system to reduce biotoxic accumulation of H 2 O 2 and helps in DNA repair.

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Section: Expression Of Csddi1 Ddr1 and Ddb1 Genes In Response To Comentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

Wang

Zhu

2020

Front. Plant Sci.

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“…A more classical pattern is well observed for other traits like plant height. Correlated genes, PgAAO and PgHK4, are involved in regulating the plant development response (plant height and nodal tiller) induced by abscisic acid (19) and in the synthesis of phylloquinone essential in photosynthesis (20), respectively. This suggests that during the vegetative phase, late-owering Sanio millet produces more tillers, grows taller and captures more light through the hormonal and photosynthesis pathways than early-owering Souna millet does.…”

Section: Genes Underlying Allelic and Traits Diversitymentioning

confidence: 99%

GWAS unveils features between early- and late-flowering pearl millets

Diack

Kanfany

Guèye

et al. 2020

Preprint

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Background: Pearl millet, a dietary food for around 100 million people in Africa and in India, has a large diversity due to an extensive genetic diversity combined with a high degree of admixture with wild relatives. In Senegal, two major morphotypes are distinguished: early-flowering and late-flowering millets. The phenotypic variabilities according to the flowering time plays an important role in pearl millet adaptation to climate variability. A better understanding of the genetic makeup of these variabilities would allow breeding of pearl millet fitting different climatic areas. In this study, we aimed to characterize the genetic basis of these phenotypic differences. Results: We defined a core collection capturing most of the diversity of cultivated pearl millet of Senegal, which includes 60 early-flowering Souna and 31 late-flowering Sanio. This panel was evaluated during the 2016 and 2017 rainy seasons at Nioro for 16 agro-morphological traits. Phenological and phenotypic traits linked with yield, flowering time, and biomass helped differentiated early- and late-flowering millets. Further, using genotyping-by-sequencing (GBS), 21,663 single nucleotide polymorphisms (SNPs) with minor allele frequencies of more than 5% were identified. Sparse Non-Negative Matrix Factorization (sNMF) analysis confirms the genetic structure in two gene pools associated with flowering time differences. Moreover, two chromosomal regions on linkage groups (LG 3) (~89.7Mb) and (LG 6) (~68.1Mb) differentiated the early-flowering into two clusters. Genome-wide association study (GWAS) was used to associate phenotypic variation to the SNPs and 18 genes were linked to flowering time, plant height, nodal tiller number, and biomass (P-value ˂ 2.3E-06). Conclusions: The diversity of early- and late-flowering pearl millet landraces of Senegal was captured using a heuristic approach. Key phenology and phenotypic traits, SNPs, and candidate genes underlying flowering time, tillering, biomass and plant height of pearl millet were identified. Chromosome rearrangements in LG3 and LG6 were implicated as a source of variation in early-flowering morphotypes. Using candidate genes underlying these features between pearl millet morphotypes would have paramount importance in breeding strategies under climate change scenarios.

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“…A more classical pattern clearly appeared in other traits, for example, plant height. Correlated genes, PgAAO and PgHK4, are involved in regulating the plant development response (plant height and nodal tillering) induced by abscisic acid (19) and in the synthesis of phylloquinone that is indispensable for photosynthesis (20), respectively. This suggests that during the vegetative phase, late-owering Sanio millet produces more tillers, grows taller and captures more light through the hormonal and photosynthesis pathways than early-owering Souna millet.…”

Section: Genes Underlying Allelic and Trait Diversitymentioning

confidence: 99%

GWAS unveils features between early- and late-flowering pearl millets

Diack

Kanfany

Guèye

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Pearl millet, a nutritious food for around 100 million people in Africa and India, displays extensive genetic diversity and a high degree of admixture with wild relatives. Two major morphotypes can be distinguished in Senegal: early-flowering Souna and late-flowering Sanio. Phenotypic variabilities related to flowering time play an important role in the adaptation of pearl millet to climate variability. A better understanding of the genetic makeup of these variabilities would make it possible to breed pearl millet to suit regions with different climates. The aim of this study was to characterize the genetic basis of these phenotypic differences.Results: We defined a core collection that captures most of the diversity of cultivated pearl millets in Senegal and includes 60 early-flowering Souna and 31 late-flowering Sanio morphotypes. Sixteen agro-morphological traits were evaluated in the panel in the 2016 and 2017 rainy seasons. Phenological and phenotypic traits related with yield, flowering time, and biomass helped differentiate early- and late-flowering morphotypes. Further, using genotyping-by-sequencing (GBS), 21,663 single nucleotide polymorphisms (SNPs) markers with more than 5% of minor allele frequencies were discovered. Sparse non-negative matrix factorization (sNMF) analysis confirmed the genetic structure in two gene pools associated with differences in flowering time. Two chromosomal regions on linkage groups (LG 3) (~89.7Mb) and (LG 6) (~68.1Mb) differentiated two clusters among the early-flowering Souna. A genome-wide association study (GWAS) was used to link phenotypic variation to the SNPs, and 18 genes were linked to flowering time, plant height, tillering, and biomass (P-value ˂ 2.3E-06).Conclusions: The diversity of early- and late-flowering pearl millet morphotypes in Senegal was captured using a heuristic approach. Key phenological and phenotypic traits, SNPs, and candidate genes underlying flowering time, tillering, biomass yield and plant height of pearl millet were identified. Chromosome rearrangements in LG3 and LG6 were inferred as a source of variation in early-flowering morphotypes. Using candidate genes underlying these features between pearl millet morphotypes will be of paramount importance in breeding for resilience to climatic variability.

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Analysis of DNA Damage-Binding Proteins (DDBs) in Arabidopsis thalian a and their Protection of the Plant from UV Radiation

Cited by 6 publications

References 0 publications

DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

GWAS unveils features between early- and late-flowering pearl millets

GWAS unveils features between early- and late-flowering pearl millets

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