Analysis of Donor Factors Affecting Human Islet Isolation With Current Isolation Protocol

Matsumoto, Shin; Zhang, G; Qualley, Sabrina A.; Clever, Jared L.; Tombrello, Y; Strong, Douglas M.; Reems, Jo Anna

doi:10.1097/00007890-200308271-00121

Cited by 13 publications

(18 citation statements)

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“…All seven transplanted patients, including single transplanted patients, had improved glycemic control and reduced insulin dosage with positive C‐peptide. It is known that the results of human islet isolation depend on the quality of donor pancreata (24,25), so it is not easy to isolate suitable human islets successfully from NHBDs. This study suggests that it should be possible to cure type 1 diabetes by means of islet transplantation using our current islet isolation method with NHBDs.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Islet Transplantation from Non-Heart Beating Donors

Noguchi

Iwanaga

Okitsu

et al. 2006

American Journal of Transplantation

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We evaluated islet transplantation from non-heart beating donors (NHBDs) with our Kyoto Islet Isolation Method. All patients had positive C-peptide after transplantation. The average HbA 1C levels of the five recipients significantly improved from 7.8 ± 0.4% at transplant to 5.2 ± 0.2% currently (p < 0.01). Three patients with no or a single autoantibody became insulin independent while the other two patients with double autoantibodies reduced their insulin requirement but did not become insulin independent. C-peptide in patients who became insulin-independent gradually increased after each transplantation whereas C-peptide in patients who did not become insulin-independent from 3 months after the first transplantation to the next transplantation dramatically decreased. The b -score of the three patients who became insulin independent was the best of eight. In conclusion, our method makes it feasible to use NHBDs for islet transplant into type 1 diabetic patients efficiently.Abbreviations: CIT, cold ischemic time; NHBD, nonheart beating donor; PFC, perfluorochemical; WIT, warm ischemic time

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Section: Discussionmentioning

confidence: 99%

Evaluation of Islet Transplantation from Non-Heart Beating Donors

Noguchi

Iwanaga

Okitsu

et al. 2006

American Journal of Transplantation

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“…Many variables, such as donor age, BMI, cause of death and the use of vasopressors, considerably influence human PIT [9,21]. This individual variability seen in humans is not observed in the experimental setting, where more standardised donors are used, highlighting the importance of donor standardisation in clinical practice [22,23]. Therefore, not all the results obtained in NHP PIT may be translated to humans and one should be cautious in generalizing these outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…Complete (less fractioned) was superior to multifractioned collection in the multivariate analysis. Most experimental [4,8,19,36] and clinical [9,22,37] studies on pancreatic isolation variables have focused on the type of gradient for islet purification and have found no differences between continuous and discontinuous methods. We used the discontinuous gradient technique, but the sample collecting method was modified during the study period.…”

Section: Discussionmentioning

confidence: 99%

Pancreatic islet isolation variables in non-human primates (rhesus macaques)

et al. 2008

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“…The characteristics of the donor may influence graft survival, in particular the donor's age, BMI, glycemia, presence of hypotension, and use of vasopressors prior to death, and eventual cause of death [14][15][16][17], with cerebrovascular stroke and intracranial hemorrhage being associated with a higher likelihood of failure [18]. Several biochemical markers have also been identified as predictors: elevations of transaminases and creatinine amongst them [19]. Shorter preservation time is also associated with a better outcome [19], which is likely a reflection of newer isolation techniques.…”

Section: Screening Criteria ▼ Donorsmentioning

confidence: 99%

Islet Cell Transplantation in Australia: Screening, Remote Transplantation, and Incretin Hormone Secretion in Insulin Independent Patients

et al. 2014

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Islet cell transplantation has emerged as a treatment modality for type 1 diabetes in the last 15 years due to the Edmonton protocol leading to consistent and sustained exogenous insulin independence post-transplantation. In recent years, consortia that involve both local and remote islet cell centers have been established, with local centers responsible for processing and shipping of islet cells, and remote centers only transplanting them. There are, however, few data on patient outcomes at remote centers. A tendency for high fasting glucose despite insulin independence was noted by us and others with an unknown mechanism. This review provides a brief history of islet cell transplantation, and focuses on the South Australian remote center experience: the challenges, screening criteria, and the impact on incretin hormone secretion of insulin independent transplant patients.

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Analysis of Donor Factors Affecting Human Islet Isolation With Current Isolation Protocol

Cited by 13 publications

References 0 publications

Evaluation of Islet Transplantation from Non-Heart Beating Donors

Evaluation of Islet Transplantation from Non-Heart Beating Donors

Pancreatic islet isolation variables in non-human primates (rhesus macaques)

Islet Cell Transplantation in Australia: Screening, Remote Transplantation, and Incretin Hormone Secretion in Insulin Independent Patients

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