Analysis of drug resistance mutations in pulmonary Mycobacterium tuberculosis isolates in the Southern coastal region of Andhra Pradesh, India

Polu, Giri Prasad; Shaik, Jasmine Mohammad; Kota, Neela Mani Kanta; Karumanchi, Deepthi; Allam, Uday Sankar

doi:10.1016/j.bjid.2019.07.002

Cited by 16 publications

(24 citation statements)

References 42 publications

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“…The acquisition of drug resistance in Mycobacterium tuberculosis (MTB) is not attributable to horizontal transfer of resistance-determining genes or regions, but instead, drug resistance results from mutations in specific resistance-determining regions of the gene targets or their promoters or in the activating enzymes of anti-TB chemotherapeutic agents (caused by nucleotide substitution, insertion, or deletion). 4 Resistance to RIF is caused by mutations in the beta subunit of DNA-dependent RNA polymerase (rpoB) and is commonly found in the 81-bp hotspot region of the rpoB gene. Meanwhile, the mechanism of resistance to isoniazid (INH) is more complex.…”

Section: Introductionmentioning

confidence: 99%

Analysis of drug resistance and mutation profiles in Mycobacterium tuberculosis isolates in a surveillance site in Beijing, China

et al. 2021

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Objective This study analyzed drug resistance and mutations profiles in Mycobacterium tuberculosis isolates in a surveillance site in Huairou District, Beijing, China. Methods The proportion method was used to assess drug resistance profiles for four first-line and seven second-line anti-tuberculosis (TB) drugs. Molecular line probe assays were used for the rapid detection of resistance to rifampicin (RIF) and isoniazid (INH). Results Among 235 strains of M. tuberculosis, 79 (33.6%) isolates were resistant to one or more drugs. The isolates included 18 monoresistant (7.7%), 19 polyresistant (8.1%), 28 RIF-resistant (11.9%), 24 multidrug-resistant (MDR) (10.2%), 7 pre-extensively drug-resistant (XDR, 3.0%), and 2 XDR strains (0.9%). A higher rate of MDR-TB was detected among previously treated patients than among patients with newly diagnosed TB (34.5% vs. 6.8%). The majority (62.5%) of RIF-resistant isolates exhibited a mutation at S531L in the DNA-dependent RNA polymerase gene. Meanwhile, 62.9% of INH-resistant isolates carried a mutation at S315T1 in the katG gene. Conclusion Our results confirmed the high rate of drug-resistant TB, especially MDR-TB, in Huairou District, Beijing, China. Therefore, detailed drug testing is crucial in the evaluation of MDR-TB treatment.

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Section: Introductionmentioning

confidence: 99%

Analysis of drug resistance and mutation profiles in Mycobacterium tuberculosis isolates in a surveillance site in Beijing, China

et al. 2021

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“…Rawat et al [9] and Vasanthakumari et al [10] stated acquired drug resistance to at least one antitubercular drug as 62.2% and 63%, respectively. Andhra Pradesh has started the 2 nd line antituberculosis treatment, that is, the DOTS plus program in the year 2006 [11].…”

Section: Discussionmentioning

confidence: 99%

Study of Resistance to First-Line Drugs in Multidrug Resistance Tuberculosis Suspects From Sputum Samples by Gold Standard – Standard Economic Variant 1% Proportion Method in North Coastal Andhra Pradesh

Mohan

Padmaja

Dusi

et al. 2022

Asian J Pharm Clin Res

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Objectives: The objectives of the study were to culture Mycobacterium tuberculosis from sputum samples of multidrug resistance (MDR) suspects on Lowenstein-Jensen (LJ) media, to test for susceptibility and resistance to first-line antitubercular drugs rifampicin (RMP), isoniazid (INH), streptomycin (SM), and ethambutol (EMB) by conventional DST on LJ media by standard economic variant proportion (1%) method and to calculate the rate of MDR and pattern of drug resistance in MDR tuberculosis (MDR-TB) suspects. Methods: A prospective, laboratory-based study was done on a total of 80 samples from cases of suspected pulmonary and extra-pulmonary TB. Identification of culture isolates as M. tuberculosis was done by susceptibility to p-nitrobenzoic acid (PNB), niacin test, and catalase activity at 68°C/pH 7. Drug sensitivity testing (DST) of M. tuberculosis cultures on LJ medium for drugs was performed by a standard economic variant of the 1% proportion method. Results: Among MDR suspects, the maximum is in the age group of 45–60 years (35%) with a mean age of 42.75±15.93 years. The sex ratio of male to female is 2:1. Out of 80 samples, 36 samples (45%) were graded 1+, 31 samples (38.7%) 2+, and 13 samples (16.3%) 3+. All 80 sputum samples showed growth on LJ media within 8 weeks. When colonies obtained on LJ medium were subjected to biochemical tests for identification, all were positive for M. tuberculosis. Out of 80 M. tuberculosis isolates tested for sensitivity against first-line drugs (except pyrazinamide), 45 (56.3%) were found to be sensitive to all four drugs (INH, RMP, EMB, and SM), and 35 (43.7%) were resistant to at least one drug. Nine isolates (11.3%) showed resistance to a single drug (monoresistance). The various MDR patterns were INH+RMP seen in 6 strains (7.5%) and INH+RMP+SM seen in 4 strains (5%). Conclusion: Drug resistance trends must be closely monitored to evaluate the efficiency of existing therapies and their effect on pulmonary tuberculosis epidemic. To design the new drug regulations, more extensive data on drug sensitivity with standardized testing protocols which is quality assured are required.

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“…MDR-TB is a growing public health threat in Zambia, and the WHO has projected that by 2021 the country will have a high burden of the disease [23,24]. DR-TB is not caused by a single factor, but by several factors which come into play and some of these include: poor adherence to TB treatment, irregular supply and stock-out of anti-TB drugs, inappropriate TB therapy, delayed diagnosis and initiation of ineff ective therapy as well as spontaneous chromosomal mutations [1,30,33,58].…”

Section: Discussionmentioning

confidence: 99%

“…Tuberculosis (TB) is one of the leading causes of death globally [1,2]. This airborne killer infectious disease causes high mortality and morbidity rates [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Drug-Resistant Mycobacterium tuberculosis in Sputum Specimens from the New and Previously Treated Tuberculosis Cases at the National Reference Chest Diseases Laboratory in Lusaka, Zambia

Mumena¹,

Kwenda²,

Ngugi³

et al. 2021

J Biomed Res Environ Sci

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Background: Drug-Resistant Tuberculosis (DR-TB) is one of the major public health issues globally. Zambia is highly burdened by TB and multi-drug resistant TB. In this study, sputum samples obtained from the new and previously treated cases of TB were examined for drug-resistant Mycobacterium tuberculosis (MTB). Methods: Sputum specimens were processed using the N-acetyl-L-cysteine-sodium hydroxide method, stained and examined using fluorescent technique and microscopy respectively. Mycobacterial DNA was extracted using the Genolyse kit, then subjected to multiplex polymerase chain reaction amplification and reverse hybridization. Drug-resistance and mutations in MTB genes were detected using the Genotype MTBDRplus VER 2.0 and MTBDRsl VER 2.0 assays. Results: A total of 329 MTB-positive sputum specimens, 102 from the new TB cases and 227 from previously treated TB cases, were analysed for drug-resistance. Among the new TB cases, 3.9% had Rifampicin (RIF) mono-resistance, 12.8% Isoniazid (INH) mono-resistance, and 17.7% had Multi-Drug Resistance (MDR). For the previously treated TB cases, 10.1% had RIF mono-resistance, 6.6% INH mono-resistance, 33.0% MDR, 1.8% poly-drug resistance, and 0.8% had pre-Extensively Drug-Resistance (pre-XDR). Mutations identified were rpoB (Ser531Leu, His526Asp, Asp516Val, His526Tyr, and Glu510His), katG (Ser315Thr 1 and Ser315Thr 2), InhA (Cys15Thr), gyrA (Ala90Val and Asp94Gly), and eis (Cys14Thr), each with a varying frequency. Conclusion: DR-TB is prevalent, especially MDR-TB, which is currently the most worrisome form of DR-TB and an emerging threat hampering efforts in the control of TB in Zambia. The early detection and effective treatment of TB cases are key in the control of DR-TB.

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Analysis of drug resistance mutations in pulmonary Mycobacterium tuberculosis isolates in the Southern coastal region of Andhra Pradesh, India

Cited by 16 publications

References 42 publications

Analysis of drug resistance and mutation profiles in Mycobacterium tuberculosis isolates in a surveillance site in Beijing, China

Analysis of drug resistance and mutation profiles in Mycobacterium tuberculosis isolates in a surveillance site in Beijing, China

Study of Resistance to First-Line Drugs in Multidrug Resistance Tuberculosis Suspects From Sputum Samples by Gold Standard – Standard Economic Variant 1% Proportion Method in North Coastal Andhra Pradesh

Molecular Detection of Drug-Resistant Mycobacterium tuberculosis in Sputum Specimens from the New and Previously Treated Tuberculosis Cases at the National Reference Chest Diseases Laboratory in Lusaka, Zambia

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