Analysis of ebastine in pharmaceutical preparations by high-performance thin-layer chromatography

Ashok, Patil Vrushali; Meyyanathan, S. N.; Pandilla, Bharani; Suresh, B.

doi:10.1556/jpc.16.2003.2.16

Cited by 10 publications

(4 citation statements)

References 2 publications

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“…In this mode, the acquisition software continuously evaluates the full-scan MS spectrum. The electrospray ionization (ESI) source conditions were set as following: sheath gas flow rate as 45 arb, Aux gas flow rate as 15 arb, capillary temperature 400 • C, full MS resolution as 70,000, MS/MS resolution as 17,500, collision energy as 20/40/60 eV in normalized collision energy (NCE) mode and spray voltage as 4.0 kV (positive) or −3.6 kV (negative), respectively (Periannan, 2003).…”

Section: Uhplc-ms/ms Metabolite Analysismentioning

confidence: 99%

Metabolic Responses of a Model Green Microalga Euglena gracilis to Different Environmental Stresses

Liu

et al. 2021

Front. Bioeng. Biotechnol.

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Euglena gracilis, a green microalga known as a potential candidate for jet fuel producers and new functional food resources, is highly tolerant to antibiotics, heavy metals, and other environmental stresses. Its cells contain many high-value products, including vitamins, amino acids, pigments, unsaturated fatty acids, and carbohydrate paramylon as metabolites, which change contents in response to various extracellular environments. However, mechanism insights into the cellular metabolic response of Euglena to different toxic chemicals and adverse environmental stresses were very limited. We extensively investigated the changes of cell biomass, pigments, lipids, and paramylon of E. gracilis under several environmental stresses, such as heavy metal CdCl2, antibiotics paromomycin, and nutrient deprivation. In addition, global metabolomics by Ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) was applied to study other metabolites and potential regulatory mechanisms behind the differential accumulation of major high-valued metabolites. This study collects a comprehensive update on the biology of E. gracilis for various metabolic responses to stress conditions, and it will be of great value for Euglena cultivation and high-value [154mm][10mm]Q7metabolite production.

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Section: Uhplc-ms/ms Metabolite Analysismentioning

confidence: 99%

Metabolic Responses of a Model Green Microalga Euglena gracilis to Different Environmental Stresses

Liu

et al. 2021

Front. Bioeng. Biotechnol.

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“…Figure 1. Chemical structure of ebastine A thorough study revealed that numerous analytical procedures for estimating ebastine in tablet and syrup dosage forms, either alone or in combination with other medications, have been described, including spectrophotometry (6)(7)(8)(9), HPTLC (10), HPLC (11)(12)(13)(14)(15)(16), and LC-MS/MS (17)(18)(19). There have been very few reports of RP-HPLC techniques (20)(21)(22) for estimating ebastine in tablet and syrup dose forms that are stable.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method applied to the quantification of anti-histaminic drug ebastine in its oral suspension dosage form

Koduru¹,

Tatapudi²,

Kalepu³

et al. 2023

Drug Anal. Res.

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For the quantification of ebastine in pharmaceutical suspension, a simple, quick, accurate, and exact stability-indicating HPLC approach was developed and validated. The drug was determined using a phase reverse system and the separation was performed in an analytical C18 column (250 mm x 4.6 mm, 5 μm). The mobile phase consists of 0.1% orthophosphoric acid and methanol in a 25:75, v/v ratio. Using a concentration range of 10–90 μg mL-1, the technique demonstrated a strong linear response (r=0.999). Effluents were measured at 262 nm while the flow rate was kept at 1.0 mL min-1. There was a retention time of 3.506 min. The method was statistically validated to determine its accuracy, precision, linearity, ruggedness, robustness, solution stability, selectivity, and forced degradation assessments. The stresses that were used were acid, alkali hydrolysis, water stress, oxidation, photolysis, and heat. Since the degradation products did not affect the capacity to identify ebastine, this technique may be taken as a stability indication. This methodology may be utilized for the analysis of Ebastine in pharmaceutical suspension, since the findings obtained were within the limits set by ICH standards.

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“…Asthmatic disease is often associated with allergic rhinitis and is a major factor affecting quality of life and escalating economic cost of treatment (Meltzer, 2001;Schoenwetter et al, 2004). Earlier reports describe a number of chromatographic methods for the determination of ebastine in biological samples (Matsuda et al, 2001;Kang et al, 2004) and in tablet formulations (Ashok et al, 2003;Prabhu et al, 2008;Sunitha et al, 2004;Ibrahim et al, 2011). Chemical structure of ebastine.…”

Section: Introductionmentioning

confidence: 99%

Crystal structure analysis of ebastine [4-(4-benzhydryloxy-1-piperidyl)-1-(4-tert-butylphenyl) butan-1-one]: an oral histamine antagonist

Sharma

Prasher²,

Tiwari

2015

J Appl Cryst

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Ebastine belongs to the category of second-generation non-sedating H1 receptor antagonists and is chiefly used for the treatment of allergic rhinitis and chronic urticaria. The present paper reports findings on the crystal and molecular structure of ebastine, employing the technique of single-crystal X-ray diffraction. Ebastine crystallizes in the monoclinic P2 1 /c space group with unitcell dimensions a = 16.5890 (12), b = 10.9575 (8), c = 16.6795 (11) Å , = 113.623 (2) , V = 2777.8 (3) Å 3 , Z = 4 and calculated density amounting to 1.123 Mg m À3 . The structure factor value was observed 1016 and final R = 0.0496 for 4360 unique reflections. The piperidine ring in the ebastine molecule was found to exist in a chair conformation, and the C-N bond length was 1.459 (3) Å , thereby showing good agreement with the standard C-N bond length of 1.472 Å .

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Analysis of ebastine in pharmaceutical preparations by high-performance thin-layer chromatography

Cited by 10 publications

References 2 publications

Metabolic Responses of a Model Green Microalga Euglena gracilis to Different Environmental Stresses

Metabolic Responses of a Model Green Microalga Euglena gracilis to Different Environmental Stresses

Stability-indicating RP-HPLC method applied to the quantification of anti-histaminic drug ebastine in its oral suspension dosage form

Crystal structure analysis of ebastine [4-(4-benzhydryloxy-1-piperidyl)-1-(4-tert-butylphenyl) butan-1-one]: an oral histamine antagonist

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