Analysis of eight different methods for the detection of <i> Helicobacter pylori</i> infection in patients with dyspepsia

Kullavanijaya, Pinit; Thong-Ngam, Duangporn; Hanvivatvong, Orrawadee; Nunthapisud, Pongpun; Tangkijvanich, Pisit; Suwanagool, Pongsapeera

doi:10.1111/j.1440-1746.2004.03506.x

Cited by 23 publications

(29 citation statements)

References 19 publications

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“…In relation to the UT, it also showed a high diagnostic capability of HpAI, although it was slightly lower than that of HE, was not significantly different, which be in accord with the described by other authors (2,4,5,11,14) . However, the UT only detected the presence of HpAI in 4% of infected patients, in whom the other two tests failed to diagnose such condition, and does not provide additional information on the status of the gastric mucosa.…”

Section: Resultssupporting

confidence: 81%

“…The analysis of the results obtained by utilization of three diagnostic methods based on gastric mucosal biopsies obtained by endoscopy, suggests that the HE stained with hematoxylin-eosin has a high diagnostic suitability, consistent with suggested by other authors, in most cases, the HpAI diagnosis can be made in appropriate gastric mucosa specimen stained with hematoxylin-eosin (1,2,5,7,11,17,19,20) , and special stains should be reserved for selected cases (1,19) . The difference to diagnose HpAI by means the HE of a single biopsy of the antrum and body mucosa, highlight the importance of obtain at least one specimen in each region of the gastric mucosa, in order to increase significantly (P<0.001) the diagnostic usefulness of the HE.…”

Section: Discussionmentioning

confidence: 82%

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Comparison of three methods based on endoscopic gastric biopsies for diagnosis of Helicobacter pylori active infection in a clinical setting

Arismendi-Morillo

Hernández

Mengual

et al. 2011

Arq. Gastroenterol.

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-Context -The correct diagnosis and effective treatment of Helicobacter pylori gastric infection are essential in controlling this infection. Objective -To compare the diagnostic value of three tests based in endoscopic gastric biopsies histopathological evaluation with hematoxylin-eosin (H-E) staining, urease rapid test and microbiological culture for detecting Helicobacter pylori active infection, in order to make recommendations for daily clinical practice. Methods -Gastric biopsies from 115 adult patients (85 female/30 male) were obtained by upper gastrointestinal endoscopy and studied by histopathological evaluation with H-E (antrum-corpus), urease test in 2 hours (antrum) and microbiological culture (antrum). Results -Helicobacter pylori active infection was diagnosed in 67% of patients. Helicobacter pylori active infection was detected by histopathological evaluation with H-E, urease test and microbiological culture in 87%, 79% and 70% of the positive cases, respectively. There were significant differences when histopathological evaluation with H-E and urease test rapid test when compared with microbiological test (P<0.01). There was no significant difference between histopathological evaluation with H-E and urease test (P = 0.7). The kappa index of agreement for histopathological evaluation with H-E/urease test was 0.56, histopathological evaluation with H-E/microbiological culture 0.6, and urease test/microbiological culture 0.64. Conclusions -In a hospital setting like the one studied, histopathological evaluation with H-E and urease test are the most recommended tests for diagnosis of Helicobacter pylori active infection based in endoscopic biopsies. If pathological information of gastric lesions will be required, histopathological evaluation with H-E is essential. Urease test is mandatory if a prompt diagnosis is necessary. Microbiological culture can be used in cases of persistent or complicated infection, which may require studies on Helicobacter virulence or antimicrobial susceptibility. Selected cases might demand a combination of several tests. The three tests exhibit a good concordance level for Helicobacter pylori active infection diagnosis. HEADINGS -Gastritis. Helicobacter infections. Urease. Microbiological technique.

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Section: Resultssupporting

confidence: 81%

Section: Discussionmentioning

confidence: 82%

Comparison of three methods based on endoscopic gastric biopsies for diagnosis of Helicobacter pylori active infection in a clinical setting

Arismendi-Morillo

Hernández

Mengual

et al. 2011

Arq. Gastroenterol.

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“…The agar plates were incubated under microaerophilic conditions (5 to 15% O 2 and 10% CO 2 ) at 37°C for 4 to 10 days (14). The identification of H. pylori was performed using catalase, oxidase, and urease tests, microscopy, and ureA gene detection (12,19).…”

mentioning

confidence: 99%

Molecular Basis of Pathogenicity in Helicobacter pylori Clinical Isolates

et al. 2010

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This study identified pathogenicity genes in 40 Helicobacter pylori clinical isolates. The cagA, vacA, and iceA genes were detected in 65%, 97.5%, and 97.5% of the isolates, respectively. The cagA, iceA1, and vacAs1a/m1 genes were related to erosive gastritis, whereas the vacAs2/m2 and iceA2 genes were associated with enanthematous gastritis.Helicobacter pylori is considered the major etiologic agent of chronic active gastritis, an essential catalyst in the emergence of peptic ulcer, and a risk factor for the development of gastric cancer (17). Studies indicate that the evolution of the infection depends in part on the expression of specific bacterial pathogenicity genes, such as cagA (cytotoxin-associated gene A), vacA (vacuolating cytotoxin), and iceA (induced by contact with epithelium) (2).The cagA gene is considered to be a marker for the presence of a cagA pathogenicity island (8). The cagA-positive H. pylori strains increase interleukin-8 production and gastric inflammation (5). The vacA gene encodes a vacuolating cytotoxin able to induce the formation of cytoplasmic vacuoles in epithelial cells (11). This gene comprises two variable regions: the signal region, with two alleles, s1 (subtypes s1a, s1b, and s1c) and s2, and the middle region, with the alleles m1 and m2 (3, 28). In general, the s1/m1 strains produce large amounts of vacuolating cytotoxin, the s1/m2 strains produce moderate amounts, and the s2/m2 strains produce little or none (3). The iceA gene has two alleles: iceA1 and iceA2. The iceA1 allele is associated with peptic ulcer, and iceA2 is related to asymptomatic gastritis (24,29).This study analyzed the presence of cagA, vacA, and iceA genes in clinical isolates and correlated these findings with the endoscopic diagnosis. Forty isolates of H. pylori were obtained from biopsy specimens of the gastric antrum collected from dyspeptic patients admitted to the upper gastrointestinal endoscopic ward in the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. This study was approved by the ethics committee of our university. Informed consent was obtained from all patients.After collection, the biopsy specimens were kept in brain heart infusion broth (Acumedia, United States) with 20% glycerol and refrigerated (4 to 8°C) for a maximum of 4 h (22). This broth was thereafter vortexed, and 200 l was added to medium Columbia agar (Oxoid, United Kingdom), supplemented with 7% sheep blood and with a selective mixture for Helicobacter species isolation (Cefar, Brazil). The agar plates were incubated under microaerophilic conditions (5 to 15% O 2 and 10% CO 2 ) at 37°C for 4 to 10 days (14). The identification of H. pylori was performed using catalase, oxidase, and urease tests, microscopy, and ureA gene detection (12,19).The DNA extraction was performed after 48 h of bacterial growth. Colonies were collected and resuspended in 500 l of 1ϫ TE buffer. The suspension was centrifuged at 10,000 ϫ g for 5 min, and the supernatant was thereafter discarded. The DNA from the clinical isolat...

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“…(1) no problem, (2) minimal problem (can be easily ignored without effort), (3) mild problem (can be ignored with effort), (4) moderate problem (cannot be ignored but does not affect daily activities), (5) moderately severe problem (cannot be ignored and occasionally limits daily activities), (6) severe problem (cannot be ignored and often limits concentration on daily activities), and (7) very severe problem (cannot be ignored, markedly limits daily activities and often requires rest). Patients were then screened for H. pylori infection by endoscopic biopsies (invasive) and C-14 urea breath test (noninvasive).…”

Section: Methodsmentioning

confidence: 99%

“…Several methods have been developed to detect H. pylori infection which include invasive tests that require endoscopy to obtain tissue biopsies, such as rapid urease test, culture, histological examination, and polymerase chain reaction and non-invasive tests such as urea breath test or stool antigen test. 6 The reported prevalence of H. pylori in patients with FD ranges from 39% to 87%, but it remains unclear whether H. pylori infection actually causes symptoms or is just an associated finding. 7,8 Many trials evaluating the role of H. pylori in FD and efficacy of H. pylori eradication on symptomatology of FD have been published in past, but most of these have given conflicting results.…”

Section: Introductionmentioning

confidence: 99%

Sociodemographic profile of Helicobacter pylori positive functional dyspepsia patients in central India

Salankar

Rode

Hemnani

et al. 2015

Int J Basic Clin Pharmacol

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Analysis of eight different methods for the detection of Helicobacter pylori infection in patients with dyspepsia

Cited by 23 publications

References 19 publications

Comparison of three methods based on endoscopic gastric biopsies for diagnosis of Helicobacter pylori active infection in a clinical setting

Comparison of three methods based on endoscopic gastric biopsies for diagnosis of Helicobacter pylori active infection in a clinical setting

Molecular Basis of Pathogenicity in Helicobacter pylori Clinical Isolates

Sociodemographic profile of Helicobacter pylori positive functional dyspepsia patients in central India

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