The generation mechanism of cutting force in ultrasonic vibration assisted turning (UAT), with the composition and decomposition of cutting force is discussed in this paper, and the model of cutting force in UAT is established based on the mechanism of UAT. The force measuring test system is designed on the basis of the established machining system of UAT. The contrast experiments for turning the workpiece of 304 austenitic stainless steel are conducted with and without ultrasonic vibration under different technological parameters. Furthermore, the relational model and correlation between technological parameters and cutting force is obtained by regression analysis and variance analysis. Thereby, the mutual relation among these technological parameters is effectively controlled, which contributes to achieving the high quality and high efficient processing. Simultaneously, the influences of single technological parameter with the interaction between technological parameters on cutting force are researched and analyzed. The results prove that the cutting force is reduced significantly with the aid of ultrasonic vibration in turning and the choice of the proper ultrasonic amplitude, there is an optimal range of ultrasonic amplitudes as well. Meanwhile, the cutting parameters have great influence on cutting force, among which depth of cut has the superior influence, then the cutting speed, and feed rate has the minimal influence. Moreover, cutting parameters should not be too large, UAT is mainly used for semi-finishing or finishing at medium-low speed. UAT will get more ideal machining effect if cutting parameters are chosen properly.