Analysis of element species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS

Mestek, Oto; Polàk, Jan; Koplík, Richard; Komínková, Jana; Šantrůček, Jiří; Kodı́ček, Milan; Kvasnička, František

doi:10.1007/s00217-006-0499-2

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…When comparing item by item, most elements shared the same order of magnitude and the exact values differed between the samples. Mestek et al (2007) detected Co, Cu, Fe, Mn, Mo, Ni, P, and Zn in buckwheat flour. In particular, P concentration was very high (3180 μg/g).…”

Section: Elements Concentrations In Different Parts Of Buckwheatmentioning

confidence: 93%

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Evaluation of essential and toxic elements concentrations in different parts of buckwheat

Yin-hui¹,

Peng²,

Liu³

et al. 2013

Czech J. Food Sci.

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Huang Y., Peng L., Liu Y., Zhang Z., Lv L., Zhao G. (2013): Evaluation of essential and toxic elements concentrations in different parts of buckwheat. Czech J. Food Sci., 31: 249-255.The elements concentrations in different parts of buckwheat and the commercial products of it were analysed by experimental and chemometric approaches. The results indicated that the essential and toxic elements concentrations were significantly different in various parts of the buckwheat, with the seeds revealing the lowest concentrations. The elemental patterns were not significantly different between Fagopyrum tataricum (L.) Gaertn and F. esculentum Moench. Abundant essential elements were found in the commercial buckwheat tea. The detection of heavy metals manifested the potential toxicity of buckwheat.

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Section: Elements Concentrations In Different Parts Of Buckwheatmentioning

confidence: 93%

“…Bonafaccia et al (2003) found that more trace elements were accumulated in the bran. Mestek et al (2007) and Zn in the extract of buckwheat flour. Other studies tested the metals concentrations in the buckwheat grain (Pongrac et al 2011).…”

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confidence: 99%

Evaluation of essential and toxic elements concentrations in different parts of buckwheat

Yin-hui¹,

Peng²,

Liu³

et al. 2013

Czech J. Food Sci.

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“…Aspartic acid and/or asparagine were also found in appreciable amounts. Aspartic acid is known to present in many metaloproteins and was found to be an important component in metal binding compounds in amaranth, buckwheat (Mestek et al, 2007), rye (Polak et (Mestek et al, 2010) and rapeseeds (Mestek et al, 2007a). All of the above mentioned amino acids are present in bread at appreciable levels: 3.7-4.3 g·kg -1…”

Section: Investigation Of the Ligands Of Trace Elementsmentioning

confidence: 99%

“…plant metallothioneins called phytochelatins (PC), which play an important role in metal binding (Cobbet, 1999;Rauser, 1999;Zenk, 1996), especially in plants exposed to high metal ions concentrations. In our previous papers (Mestek et al, 2007(Mestek et al, , 2007a(Mestek et al, , 2010Polak et al, 2009), we pointed out that peptides rich in aspartic and glutamic acids can also take part in metal binding in plants. However, it should be noted that peptides are not the only metal-binding compounds.…”

Section: Introductionmentioning

confidence: 99%

Analyses of trace metals, peptide ligands of trace metals and mercury speciation in home prepared bread

Mestek

Komínková

Šantrůček

et al. 2012

Chemical Speciation & Bioavailability

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Bread represents an important source of trace elements in the human diet. This study is focused on home prepared bread in the Czech Republic. The amounts of Cu, Mo, Mn, Ni and Zn (total and soluble in Tris-HCl buffer, pH 7.5), Cd, Co, Fe, Pb and Tl (total only) as well as Hg (total and soluble in mercaptoethanol-HCl mixture) in raw materials and baked bread were determined using ICP-MS. Moreover, the speciation of elements was investigated using HPLC/ICP-MS. Isolated peptide ligands of the trace elements were analysed for amino acids and characterised by MALDI-MS. The concentrations of all elements were in accordance with Czech legislation. The solubility of the Ni species was not affected by the baking process, whereas the solubilities of Mo, Mn and Zn species decreased. Soluble mercury was found only in the inorganic form. The soluble species of Cu, Mo, Mn, Ni and Zn were found in two fractions with the apparent molecular weights of 1-2 kDa and 4-5 kDa. Ligands of trace metals isolated from these fractions contained appreciable amounts of Asx, Glx, Gly, Ser and Cys. No phytochelatin-like peptides were found in the MALDI-MS spectra of isolated ligands,. Using MALDI-MS/MS, the partial amino-acids sequences of peptide ligands were obtained, and the linkages of peptides and saccharides confirmed. The MS analysis of the trypsin digest of the medium molecular weight fraction revealed several proteins rich in cysteine (e.g., barwin and amylase inhibitors).

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“…Our previous experiments proved that soluble low molecular binding compounds of Cu, Zn, and other metals in buckwheat, amaranth (Mestek et al 2007a), and rape (Mestek et al 2007b) are rich in dicarboxylic amino acids and do not contain PCs. In the present work an analogous approach to the element species fractionation based on size exclusion chromatography -inductively coupled plasma mass spectrometry was applied for the analysis of rye flour and tissues of rye plants cultivated hydroponically both in normal fertiliser solution and in cadmium-enriched one.…”

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confidence: 92%

Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings

Polàk¹,

Mestek²,

Koplík³

et al. 2009

Czech J. Food Sci.

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Analysis of element species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS

Cited by 10 publications

References 24 publications

Evaluation of essential and toxic elements concentrations in different parts of buckwheat

Evaluation of essential and toxic elements concentrations in different parts of buckwheat

Analyses of trace metals, peptide ligands of trace metals and mercury speciation in home prepared bread

Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings

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