Analysis of embryonic proteome modulation by GA and ABA from germinating rice seeds

Kim, Sun Tae; Kang, Suna; Wang, Yiming; Kim, Pil Joo; Hwang, Du Hyeon; Kang, Kyu Young

doi:10.1002/pmic.200800183

Cited by 73 publications

(74 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Its function is not entirely known yet. The results obtained in this work are not consistent with the results of Yano et al (1995) or Kim et al (2008), who detected an increase of NDPK during germination and seedling growth. However, Harris et al (1994) detected the up-regulation of NDPK as a result of stress response, which suggests that the role of NDPK in plants is diversified and needs further investigation.…”

Section: Metabolismcontrasting

confidence: 99%

Influence of exogenous abscisic acid on alterations in protein expression in the proteome of Triticosecale seedlings

et al. 2012

View full text Add to dashboard Cite

The aim of this study was to investigate molecular basis of germination inhibition of Triticosecale under the influence of exogenous ABA. Seedlings were isolated from seeds after 48 h of germination in water (control sample) and 100 lM ABA solution. It was observed that the applied concentration of phytohormone caused a significant inhibition of germination and a suppressed contribution of polysomes in the total ribosomal fraction. To identify proteins whose expression was affected by ABA, a proteomic approach employing 2D electrophoresis was used. Proteome maps for both control and ABA-treated samples displayed over 2,200 Coomassie Brilliant Blue-stained spots each. Protein spots showing either increase or decrease in spot intensity under the ABA influence were identified by mass spectrometry. Many of the identified proteins whose expression was stimulated by ABA proved to be stress-related, involved in nucleotide metabolism or playing a regulatory and signal transduction role, whereas proteins whose expression decreased are known to be involved in numerous metabolic pathways (including energy, carbohydrate or nucleotide metabolism) as well as related to genetic information processing and protein synthesis. Our results show that the germination inhibition caused by ABA is a result of multigene, diversified actions leading together to triticale growth suppression.

show abstract

Section: Metabolismcontrasting

confidence: 99%

Influence of exogenous abscisic acid on alterations in protein expression in the proteome of Triticosecale seedlings

et al. 2012

View full text Add to dashboard Cite

show abstract

“…However, Kaur et al (1998) found that GA 3 was more effective than CTK in enhancing the reduced germination and seedling growth of chickpea seeds under salt stress. GA synthesis affects not only alterations in protein expression, but also increases in germination rate, promotes root and shoot length during seed germination, and promotes early seedling development (Gallardo et al, 2002;Kim et al, 2008). In the present study, GA content was significantly reduced and seed germination time was delayed by salt.…”

Section: Phytohormonesmentioning

confidence: 48%

“…Recent evidence has suggested a role for the ubiquitin-proteasome pathway in CTK, GA, and ABA signalings (Itoh et al, 2003). 26S ubiquitin-proteasome regulatory subunit 4 homolog levels were increased during rice seed germination by GA and ABA (Kim et al, 2008). Spots 1, 3, 14, and 16 were up-regulated by salt in N2899 and were unaffected in Lee68.…”

Section: Differentially Expressed Proteinsmentioning

confidence: 86%

See 1 more Smart Citation

Proteomic analysis of seed germination under salt stress in soybeans

Fan

Zheng

et al. 2011

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop, and its production is severely affected by saline soils. Therefore, the response of soybean seeds to salt stress during germination was investigated at both physiological and proteomic levels. The salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed coat. In both cultivars, the final germination percentage was not affected by salt, but the mean germination times of Lee68 and N2899 were delayed by 0.3 and 1.0 d, respectively, compared with controls. In response to salt stress, the abscisic acid content increased, and gibberellic acid (GA 1+3 ) and isopentenyladenosine decreased. Indole-3-acetic acid increased in Lee68, but remained unchanged in N2899. The proteins extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE), followed by Coomassie brilliant blue G-250 staining. About 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7 were reproducibly resolved, of which 18 protein spots showed changes in abundance as a result of salt stress in both cultivars. After matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially expressed proteins, the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successfully identified. Ferritin and 20S proteasome subunit β-6 were up-regulated in both cultivars. Glyceraldehyde 3-phosphate dehydrogenase, glutathione S-transferase (GST) 9, GST 10, and seed maturation protein PM36 were down-regulated in Lee68 by salt, but still remained at a certain level. However, these proteins were present in lower levels in control N2899 and were up-regulated under salt stress. The results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean seed germination.

show abstract

“…Proteomics have been applied to investigate seed germination in many species, such as Arabidopsis (Arabidopsis thaliana; Gallardo et al, 2001Gallardo et al, , 2002Galland et al, 2014), rice (Oryza sativa; Yang et al, 2007), and pea (Pisum sativum; Wang et al, 2012b). These studies focused on seed germination at the optimal temperature under normal conditions or in response to chemical treatments, such as GA (Gallardo et al, 2002;Kim et al, 2008), ABA (Kim et al, 2008), and salicylic acid (Rajjou et al, 2006), and environmental stress, such as salinity (Yacoubi et al, 2011(Yacoubi et al, , 2013. To the best of our knowledge, no proteomic study has examined the effect of temperature on seed germination.…”

mentioning

confidence: 99%

Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation

et al. 2015

View full text Add to dashboard Cite

Germination and thermoinhibition in lettuce (Lactuca sativa 'Jianyexianfeng No. 1') seeds were investigated by a proteomic comparison among dry seeds, germinated seeds at 15°C, at 15°C after imbibition at 25°C for 48 h, or at 25°C in KNO 3 (all sampled individually at germination), and ungerminated seeds at 25°C, a thermoinhibitory temperature. Before twodimensional gel electrophoresis analysis, storage proteins (greater than 50% of total extractable protein) were removed by polyethylene glycol precipitation, which significantly improved the detection of less abundant proteins on two-dimensional gels. A total of 108 protein spots were identified to change more than 2-fold (P , 0.05) in abundance in at least one germination treatment. Nineteen proteins increasing and one protein decreasing in abundance during germination had higher abundance in germinated 15°C, 15°C after imbibition at 25°C for 48 h, and 25°C in KNO 3 seeds than in ungerminated 25°C seeds. Gene expression of 12 of those proteins correlated well with the protein accumulation. Methionine metabolism, ethylene production, lipid mobilization, cell elongation, and detoxification of aldehydes were revealed to be potentially related to lettuce seed germination and thermoinhibition. Accumulation of three proteins and expression of five genes participating in the mevalonate (MVA) pathway of isoprenoid biosynthesis correlated positively with seed germinability. Inhibition of this pathway by lovastatin delayed seed germination and increased the sensitivity of germination to abscisic acid. MVA pathway-derived products, cytokinins, partially reversed the lovastatin inhibition of germination and released seed thermoinhibition at 25°C. We conclude that the MVA pathway for isoprenoid biosynthesis is involved in lettuce seed germination and thermoinhibition.Seed germination is a complex process that is influenced by many environmental factors, such as light, temperature, and moisture (Bewley et al., 2013). Temperature is the most important environmental factor in regulating seed germination. For a given species, there is an optimal temperature or temperature range for seed germination, below and above which seed germination will be delayed or inhibited. The failure of a seed to germinate at supraoptimal temperatures is commonly called thermoinhibition (Reynolds and Thompson, 1971;Abeles, 1986). In nature, thermoinhibition of seed germination is an adaptive regeneration strategy for the seeds of winter annual plants, which prevents the seeds germinating during the summer when the conditions are not appropriate for seedling growth (Baskin and Baskin, 1998;Allen et al., 2007). However, thermoinhibition often creates a problem for crop production. It causes delayed and poor germination of crop species with low optimal temperatures for seed germination and thus limits the time and region for crop cultivation. In the case of lettuce (Lactuca sativa) seeds, which exhibit thermoinhibition at 25°C to 30°C depending on variety and production environment (Guzman et al., 1992...

show abstract

Analysis of embryonic proteome modulation by GA and ABA from germinating rice seeds

Cited by 73 publications

References 39 publications

Influence of exogenous abscisic acid on alterations in protein expression in the proteome of Triticosecale seedlings

Influence of exogenous abscisic acid on alterations in protein expression in the proteome of Triticosecale seedlings

Proteomic analysis of seed germination under salt stress in soybeans

Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation

Contact Info

Product

Resources

About