Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019

Wang, Jing; Meng, Min; Xu, Huan; Wang, Ting; Liu, Ying; Yan, Han; Liu, Peiman; Qin, Daogang; Yang, Qian

doi:10.1186/s12879-021-06112-9

Cited by 10 publications

(12 citation statements)

References 57 publications

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“…Within the EVSurv, mainly stool samples or CSF from patients with polio-compatible symptoms, i.e., suspected aseptic meningitis/encephalitis or AFP, are tested for the presence of enteroviruses. Since 2006, approximately 25% of the samples have been enterovirus positive each year, which is in line with data from other countries [69][70][71][72][73], although data vary due to differences in study design, case definitions, sample type and age groups considered [71,74,75]. In recent years annual sample numbers and overall positivity rate have slightly decreased in Germany.…”

Section: Discussionsupporting

confidence: 76%

Enterovirus Surveillance (EVSurv) in Germany

Keeren

Böttcher²,

Diedrich

2021

Microorganisms

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The major aim of the enterovirus surveillance (EVSurv) in Germany is to prove the absence of poliovirus circulation in the framework of the Global Polio Eradication Program (GPEI). Therefore, a free-of-charge enterovirus diagnostic is offered to all hospitals for patients with symptoms compatible with a polio infection. Within the quality proven laboratory network for enterovirus diagnostic (LaNED), stool and cerebrospinal fluid (CSF) samples from patients with suspected aseptic meningitis/encephalitis or acute flaccid paralysis (AFP) are screened for enterovirus (EV), typing is performed in all EV positive sample to exclude poliovirus infections. Since 2006, ≈200 hospitals from all 16 German federal states have participated annually. On average, 2500 samples (70% stool, 28% CSF) were tested every year. Overall, the majority of the patients studied are children <15 years. During the 15-year period, 53 different EV serotypes were detected. While EV-A71 was most frequently detected in infants, E30 dominated in older children and adults. Polioviruses were not detected. The German enterovirus surveillance allows monitoring of the circulation of clinically relevant serotypes resulting in continuous data about non-polio enterovirus epidemiology.

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Section: Discussionsupporting

confidence: 76%

Enterovirus Surveillance (EVSurv) in Germany

Keeren

Böttcher²,

Diedrich

2021

Microorganisms

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“…Unfortunately, in this study, the number of days between symptom onset and sampling was not available. In line with previous studies [ 9 , 12 ], our findings showed that the incidence of aseptic meningitis by EV in males is higher than in females, although the difference is not statistically significant. As shown in previous studies, most cases of aseptic meningitis were observed in summer and autumn.…”

Section: Discussionsupporting

confidence: 92%

Molecular characterization of enterovirus detected in cerebrospinal fluid and wastewater samples in Monastir, Tunisia, 2014–2017

Rmadi

Elargoubi

González-Sanz

et al. 2022

Virol J

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Background Enteroviruses (EVs) are considered the main causative agents responsible for aseptic meningitis worldwide. This study was conducted in the Monastir region of Tunisia in order to know the prevalence of EV infections in children with meningitis symptoms. Detected EV types were compared to those identified in wastewater samples. Methods Two hundred CSF samples collected from hospitalized patients suspected of having aseptic meningitis for an EV infection between May 2014 and May 2017 and 80 wastewater samples collected in the same time-period were analyzed. EV detection and genotyping were performed using PCR methods followed by sequencing. Phylogenetic analyses in the 3′-VP1 region were also carried-out. Results EVs were detected in 12% (24/200) CSF and in 35% (28/80) wastewater samples. EV genotyping was reached in 50% (12/24) CSF-positive samples and in 64% (18/28) sewage. Most frequent types detected in CSF were CVB3, E-30 and E-9 (25% each). In wastewater samples, the same EVs were identified, but also other types non-detected in CSF samples, such as E-17,CVA9 and CVB1 from EV species B, and EV-A71 and CVA8 from EV-A, suggesting their likely lower pathogenicity. Phylogenetic analysis showed that within the same type, different strains circulate in Tunisia. For some of the EV types such as E-9, E-11 or CVB3, the same strains were detected in CSF and wastewater samples. Conclusions Epidemiological studies are important for the surveillance of the EV infections and to better understand the emergence of certain types and variants.

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“…The differences in prevalence between the different studies could be attributed to the test method and population of patients, and factors such as age group, sampling time, sample storage and transport, season, sporadic cases or an outbreak, and the high variability of the viral genes or other viral infections. [ 17 , 18 ] In this study, the most frequent cause of viral meningitis was EVs infection, followed by VZV and HSV infection. In America, in addition to EVs and various arboviruses, HSV and VZV have also been frequently isolated in patients with aseptic meningitis.…”

Section: Discussionmentioning

confidence: 78%

Epidemiological and etiological characteristics of viral meningitis for hospitalized pediatric patients in Yunnan, China

Liu

Zhang

et al. 2022

Medicine

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Background: Viral infection is the most common cause of aseptic meningitis. The purpose of this study was to identify the viruses responsible for aseptic meningitis to better understand the clinical presentations of this disease. Method: Between March 2009 and February 2010, we collected 297 cerebrospinal fluid specimens from children with aseptic meningitis admitted to a pediatric hospital in Yunnan (China). Viruses were detected by using “in house” real-time quantitative polymerase chain reaction or reverse-transcription real-time quantitative polymerase chain reaction from these samples. Phylogenetic analyses were conducted using the Molecular Evolutionary Genetic Analysis version 7.0 software, with the neighbor-joining method. Results: Viral infection was diagnosed in 35 of the 297 children (11.8%). The causative viruses were identified to be enteroviruses in 25 cases (71.4%), varicella-zoster virus in 5 cases (14.3%), herpes simplex virus 1 in 2 cases (5.7%), and herpes simplex virus 2, Epstein–Barr virus, and human herpesvirus 6 in 1 case each (2.9% each). Of the enteroviruses, coxsackievirus B5 was the most frequently detected serotype (10/25 cases; 40.0%) and all coxsackievirus B5 strains belonged to C group. Conclusions: In the study, a causative virus was only found in the minority of cases, of them, enteroviruses were the most frequently detected viruses in patients with viral meningitis, followed by varicella-zoster virus and herpes simplex virus. Our findings underscore the need for enhanced surveillance and etiological study of aseptic meningitis.

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Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019

Cited by 10 publications

References 57 publications

Enterovirus Surveillance (EVSurv) in Germany

Enterovirus Surveillance (EVSurv) in Germany

Molecular characterization of enterovirus detected in cerebrospinal fluid and wastewater samples in Monastir, Tunisia, 2014–2017

Epidemiological and etiological characteristics of viral meningitis for hospitalized pediatric patients in Yunnan, China

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