Analysis of Enzyme Activities of the Gut Bacterial Communities in Labeo rohita fed Differentially Treated Animal Fleshing Diets

Sumathi, C.; Priya, D. Mohana; Babu, V.; Sekaran, G.

doi:10.4172/1948-5948.1000061

Cited by 5 publications

(3 citation statements)

References 78 publications

(77 reference statements)

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“…Before the present work, only two groups (Mountfort et al, 1993;Sasmal and Ray, 2015) described the presence of pectinolytic activity in bacteria isolated from the gut of mullet and Tilapia. In general, the percentage of bacteria showing extracellular lipolytic and proteolytic activities in this work is pretty similar to that reported in other studies (Gatesoupe et al, 1997;Sumathi et al, 2011;Ariole et al, 2014;Tiwari et al, 2015). However, findings regarding the percentage of cellulolytic and amylolytic strains are quite different from those in a number of previous reports which described higher proportions of these isolates even in carnivorous fishes (Das and Tripathi, 1991;Sugita et al, 1997; FIGURE 2 | Phylogenetic tree constructed based on partial 16S rRNA gene sequences indicating evolutionary relationship of the enzyme-producing isolates among themselves and with their nearest type strains.…”

Section: Discussionsupporting

confidence: 92%

Hydrolytic Exoenzymes Produced by Bacteria Isolated and Identified From the Gastrointestinal Tract of Bombay Duck

et al. 2020

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Bacteria producing hydrolytic exoenzymes are of great importance considering their contribution to the host metabolism as well as for their various applications in industrial bioprocesses. In this work hydrolytic capacity of bacteria isolated from the gastrointestinal tract of Bombay duck (Harpadon nehereus) was analyzed and the enzyme-producing bacteria were genetically characterized. A total of twenty gutassociated bacteria, classified into seventeen different species, were isolated and screened for the production of protease, lipase, pectinase, cellulase and amylase enzymes. It was found that thirteen of the isolates could produce at least one of these hydrolytic enzymes among which protease was the most common enzyme detected in ten isolates; lipase in nine, pectinase in four, and cellulase and amylase in one isolate each. This enzymatic array strongly correlated to the previously reported eating behavior of Bombay duck. 16S rRNA gene sequence-based taxonomic classification of the enzyme-producing isolates revealed that the thirteen isolates were grouped into three different classes of bacteria consisting of eight different genera. Staphylococcus, representing ∼46% of the isolates, was the most dominant genus. Measurement of enzyme-production via agar diffusion technique revealed that one of the isolates which belonged to the genus Exiguobacterium, secreted the highest amount of lipolytic and pectinolytic enzymes, whereas a Staphylococcus species produced highest proteolytic activity. The Exiguobacterium sp. expressing a maximum of four hydrolases, appeared to be the most promising isolate of all.

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Section: Discussionsupporting

confidence: 92%

Hydrolytic Exoenzymes Produced by Bacteria Isolated and Identified From the Gastrointestinal Tract of Bombay Duck

et al. 2020

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“…Protease activity was exhibited by a majority of the isolates (72.2%) reflecting that the bacterial flora associated with the intestinal tract of Clarias gariepinus are capable of digesting foods rich in proteins. Some authors have also established that bacteria in the digestive tract of fish demonstrated proteolytic, lipolytic, amylolytic and cellulolytic activities Saha et al, 2006;Ray et al, 2010;Sumathi et al, 2011 andKalu, 2013). The presence of these microorganisms in the digestive tract indicates a significant role played by them during digestion of food.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Activities of Intestinal Bacteria Isolated from Farmed <i>Clarias gariepinus</i>

Ariole¹,

Nwogu²,

Chuku³

2014

ija

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The culturable bacteria associated with the digestive tract of a freshwater cultured fish, Clarias gariepinus, and their degradative abilities were established. The spread plate method was employed for bacterial isolation. The bacterial isolates were qualitatively screened for extracellular enzyme-producing ability using milk agar, starch agar, egg yolk agar and cellulose agar for protease, amylase, lipase and cellulase activities respectively. A total of 18 bacterial isolates were identified. Bacteria of the genera Bacillus, Staphylococcus, Vibrio, Aeromonas, Pseudomonas, Lactobacillus, Escherichia, Salmonella, Enterobacter, Micrococcus and Flavobacterium were isolated from fish digestive tract at different frequencies with Bacillus predominating. Enzymatic studies indicated that the bacterial isolates possess the ability to degrade proteins, starch, lipids and cellulose. The percentage composition of enzyme-producing bacteria are -protease producing strains (72.2%), lipase producing strains (61.1%), amylase producing strains (55.6%) and cellulose producing strains (38.9%). All the isolates possessed multienzyme activity. An isolate (Bacillus sp. B 1 ) showed activity for protease, amylase, lipase and cellulase enzymes. Therefore, the isolated indigenous multiple enzyme-producing strains can be effectively exploited for use as probiotics while formulating aquafeeds.

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“…The gut microbiota vested with diverse enzymatic potentials plays a crucial role in the improvement of the host metabolism, the repression of pathogens, the contribution of additional nutrients and the improvement of nutrient digestibility and absorption [ 50 , 51 ]. Extracellular enzymes, such as lipase, protease and amylase, among others, produced by most lactic acid bacteria, play an important role in the guts of animals and human beings, including infants [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Distribution of Important Probiotic Genes and Identification of the Biogenic Amines Produced by Lactobacillus acidophilus PNW3

Alayande

Aiyegoro

Ateba

2020

Foods

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The genome of Lactobacillus acidophilus PNW3 was assessed for probiotic and safety potentials. The genome was completely sequenced, assembled using SPAdes, and thereafter annotated with NCBI prokaryotic genome annotation pipeline (PGAP) and rapid annotation using subsystem technology (RAST). Further downstream assessment was determined using appropriate bioinformatics tools. The production of biogenic amines was confirmed through HPLC analysis and the evolutionary trend of the strain was determined through the Codon Tree pipeline. The strain was predicted as a non-human pathogen. A plethora of encoding genes for lactic acids and bioactive peptides production, adhesion molecules, resistance to the harsh gut environmental conditions, and improvement of the host metabolism, which are putative for important probiotic functionalities, were located at different loci within the genome. A bacteriocin predicted to be helveticin J was identified as one of the secondary metabolites. The maximum zone of inhibition exhibited by the crude bacteriocin against STEC E. coli O177 was 21.7 ± 0.58 mm and 24.3 ± 1.15 mm after partial purification (250 µg/mL). Three coding sequences were identified for insertion sequences and one for the CRISPR-Cas fragment. The protein-encoding sequence for Ornithine decarboxylase was found within the genome. L. acidophilus PNW3 presents important features categorizing it as a viable and safe probiotic candidate, though further safety investigations are necessary. The application of probiotics in livestock-keeping would ensure improved public health and food security.

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Analysis of Enzyme Activities of the Gut Bacterial Communities in Labeo rohita fed Differentially Treated Animal Fleshing Diets

Cited by 5 publications

References 78 publications

Hydrolytic Exoenzymes Produced by Bacteria Isolated and Identified From the Gastrointestinal Tract of Bombay Duck

Hydrolytic Exoenzymes Produced by Bacteria Isolated and Identified From the Gastrointestinal Tract of Bombay Duck

Enzymatic Activities of Intestinal Bacteria Isolated from Farmed <i>Clarias gariepinus</i>

Distribution of Important Probiotic Genes and Identification of the Biogenic Amines Produced by Lactobacillus acidophilus PNW3

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