Analysis of estrogenic compounds in environmental and biological samples by liquid chromatography–tandem mass spectrometry with stable isotope-coded ionization-enhancing reagent

Development of versatile isotopic labeling reagents for profiling the amine submetabolome by liquid chromatography–mass spectrometry

“…To date, several CIL reagents have been developed to quantify organic amines, carboxylic acids, carbonyl compounds and some other types of metabolites with varying degrees of performance [1,4,5,[7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. These research activities are mainly driven by the need of dealing with different classes of metabolites; a universally applicable derivatization reagent for LC-MS is not available at the present.…”

Measurement of Estradiol in Human Serum by LC-MS/MS Using a Novel Estrogen-Specific Derivatization Reagent

“…While ionization of E2 can be achieved with all the common commercially available ion sources (electrospray, atmospheric pressure chemical ionization, atmospheric pressure photoionization), 9 several methods have been described that employ chemical derivatization of E2 with structures having higher gas phase proton affinity or a permanent charge. [10][11][12][13][14][15][16][17] One particularly common reagent for E2 derivatization is dansyl chloride, a traditional fluorescent label for amino acids and proteins. [18][19][20][21][22] An advantage of dansyl derivatization of E2 is that it can be performed in an aqueous reaction solvent under mild conditions, which also makes it possible to inject the reaction mixture directly into a reversed phase column.…”

Rapid analysis of biogenic amines from rice wine with isotope-coded derivatization followed by high performance liquid chromatography–tandem mass spectrometry

“…S2) (Zhang, You, Ning, Song, & Suo, 2013). BAs standards, including TRY, PUT, HIS, CAD, PHE, TYR, and HEX, were bought from Sigma Chemical Co. with higher purity.…”