Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation

Abstract

<jats:title>Abstract</jats:title> <jats:p><jats:bold>Background: </jats:bold>Pancreatic cancer is one of the most malignant tumors. However, radiotherapy can lead to tumor recurrence, which is caused by the residual surviving cells repopulation stimulated by some molecular released from dying cells. Exosomes may mediate cell-cell communication and transfer kinds of signals from the dying cells to the surviving cells for stimulating tumor repopulation. Circular RNAs (circRNAs) may be one vital kind of exosomal cargos involving in modulating cancer cell repopulation.<jats:bold>Methods: </jats:bold>Next generation sequencing (NGS) and bioinformatics were performed to analyze and annotate the expression and function of exosome-derived circRNAs in pancreatic cancer cells after radiation. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing results.<jats:bold>Results: </jats:bold>In this study, 3,580 circRNAs were annotated in literatures and circBase among 12,572 identified circRNAs. There were 196 filtered differentially expressed circRNAs (the up-regulation and down-regulation respectively is 182 and 14, fold change &gt;2, p-value &lt;0.05). Regulation of metabolic process and lysine degradation were the main enriched biological processes and pathway according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. <jats:bold>Conclusions: </jats:bold>The hsa_circ_0002130-hsa_miR_4482-3p-NBN interaction network suggested potential sponging miRNA and target mRNA. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell repopulation upon irradiation.</jats:p>

The Potent Regulatory Role of Circular RNAs in Breast Cancer Development, Diagnosis and Treatment: An Update

“…Indeed, circATRNL1 could bind to miR-23a-3p to enhance PTEN expression, indicating the function of circATRNL1 in moderating the radiosensitivity of OSCC [85]. In addition, the association between circRNAs and radiotherapy has also been reported for other human cancers, such as hepatocellular and nasopharyngeal carcinoma, pancreatic and colorectal cancer and also glioma [86][87][88][89][90]. In general, these findings emphasize that circRNAs can be an important regulator in cancer responsiveness to radiotherapy; therefore it may also be proposed their possible involvement in radiation resistance or augmentation of radiosensitivity in BC.…”