Analysis of expressed sequence tags from the venom ducts of Conus striatus: focusing on the expression profile of conotoxins

Pi, Canhui; Liu, Yun; Peng, Chengwei; Jiang, Xiuhua; Liu, Junliang; Xu, Bin; Yu, Xuesong; Yu, Yanghong; Jiang, Xiaoyu; Wang, Lei; Dong, Meiling; Chen, Shangwu; An, Xiaobo

doi:10.1016/j.biochi.2005.08.001

Cited by 46 publications

(34 citation statements)

References 39 publications

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“…Thus, hundreds of Conus peptides have been discovered using this “transcriptomics” approach (e.g., Conticello et al, 2001; Garrett et al, 2005; Holford et al, 2009; Peng et al, 2006, 2007; Pi et al, 2006a, b; Liu et al, 2009). The advent of the next generation sequencing technologies (Margulies et al, 2005; Schuster, 2008), however, provides a means for accelerating the transcriptomics-based approach.…”

Section: Introductionmentioning

confidence: 99%

Novel venom peptides from the cone snail Conus pulicarius discovered through next-generation sequencing of its venom duct transcriptome

et al. 2012

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The venom peptides (i.e., conotoxins or conopeptides) that species in the genus Conus collectively produce are remarkably diverse, estimated to be around 50,000 to 140,000, but the pace of discovery and characterization of these peptides have been rather slow. To date, only a minor fraction have been identified and studied. However, the advent of next-generation DNA sequencing technologies has opened up opportunities for expediting the exploration of this diversity. The whole transcriptome of a venom duct from the vermivorous marine snail C. pulicarius was sequenced using the 454 sequencing platform. Analysis of the data set resulted in the identification of over eighty unique putative conopeptide sequences, the highest number discovered so far from a Conus venom duct transcriptome. More importantly, majority of the sequences were potentially novel, many with unexpected structural features, hinting at the vastness of the diversity of Conus venom peptides that remains to be explored. The sequences represented at least 14 major superfamilies/types (disulfide- and non-disulfide-rich), indicating the structural and functional diversity of conotoxins in the venom of C. pulicarius. In addition, the contry-phans were surprisingly more diverse than what is currently known. Comparative analysis of the O-superfamily sequences also revealed insights into the complexity of the processes that drive the evolution and diversification of conotoxins.

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Section: Introductionmentioning

confidence: 99%

Novel venom peptides from the cone snail Conus pulicarius discovered through next-generation sequencing of its venom duct transcriptome

et al. 2012

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“…Marine cone snails from the genus Conus are estimated to contain up to 800 species [1], each has evolved an apparatus that synthesize, store and eject a unique set of venomous cocktail contains molecular-diversified components, could reach 100 components in a single crude venom, almost all of them are short peptides (10-40 amino acids) known generally as conopeptides [2][3][4][5][6]. Conus species use their venoms not only in predation but also in defense and competition purposes.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice

Abou-Elezz¹

2017

IJEE

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Abstract:The genus Conus is equipped with a unique venomous mixture of conopeptides which secreted for predation and defense purposes. This work is aiming to explore and determine the effect of the crude venom of Conus flavidus, a wormhunting cone snail inhabiting the Red Sea, on the oxidant/ antioxidant system in mice using some oxidative stress biomarker assays. In addition to assess its histopathological effects on some treated organs. The LC 50 were detected for the crude venom using the hemolytic assay (16.7 mg/ml) and male albino mice were injected intraperitoneally with ½ LC 50 (8.3 mg/kg B.Wt). Biochemically, after 2, 4, 6, 12, 24 hours of injection the results revealed significant inhibition of superoxide dismutase (SOD) and catalase (CAT) activities in blood and liver in almost all time intervals comparing with control one. However, it showed elevation in lipid peroxide content (LPC), protein carbonyl content (PCC), nitric oxide level (NO) reduced glutathione content (GSH) and total antioxidant capacity (TAC) contents of both blood and liver in almost all time intervals. Histopathologicaly, liver and heart were dissected after 1, 3 and 7 days of injection. The treated liver showed vacuolar degeneration, karyolosis and pyknosis, mild blood sinusoidal congestion and centrilobular necrosis. The treated heart illustrated degenerated myofibrils, pyknosis, edema, blood vessel congestion, loss of striation normal construction and fascicular pattern in the myocardium. These results revealed that C. flavidus crude venom has distinct effects upon the oxidant/antioxidant cellular system and degenerative pathological effects in some tissues of treated animals, proving that this venom may contain bioactive peptides, which could be purified and used for further pharmacological and drug discovery investigations in the future.

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“…cDNA-based approaches also led to the discovery of peptides within the salivary gland of Conus pulicarius [111]. Other future science group Cone snail venomics Review sequence-based approaches such as gene expression profiling and Expressed Sequence Tag (EST) analyses were used to study the evolution of conotoxins in Conus litteratus and Conus striatus [112,113]. However, these methods suffer from a number of limitations.…”

Section: Sequence-based Discovery Of Conotoxinsmentioning

confidence: 98%

Cone Snail Venomics: From Novel Biology to Novel Therapeutics

et al. 2014

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Peptide neurotoxins from cone snails called conotoxins are renowned for their therapeutic potential to treat pain and several neurodegenerative diseases. Inefficient assay-guided discovery methods have been replaced by high-throughput bioassays integrated with advanced MS and next-generation sequencing, ushering in the era of 'venomics'. In this review, we focus on the impact of venomics on the understanding of cone snail biology as well as the application of venomics to accelerate the discovery of new conotoxins. We also discuss the continued importance of medicinal chemistry approaches to optimize conotoxins for clinical use, with a descriptive case study of MrIA featured.

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Analysis of expressed sequence tags from the venom ducts of Conus striatus: focusing on the expression profile of conotoxins

Cited by 46 publications

References 39 publications

Novel venom peptides from the cone snail Conus pulicarius discovered through next-generation sequencing of its venom duct transcriptome

Novel venom peptides from the cone snail Conus pulicarius discovered through next-generation sequencing of its venom duct transcriptome

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice

Cone Snail Venomics: From Novel Biology to Novel Therapeutics

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Analysis of expressed sequence tags from the venom ducts of Conus striatus: focusing on the expression profile of conotoxins

Cited by 46 publications

References 39 publications

Novel venom peptides from the cone snail Conus pulicarius discovered through next-generation sequencing of its venom duct transcriptome

Novel venom peptides from the cone snail Conus pulicarius discovered through next-generation sequencing of its venom duct transcriptome

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail &lt;i&gt;Conus flavidus&lt;/i&gt; on &lt;i&gt;albino&lt;/i&gt; Mice

Cone Snail Venomics: From Novel Biology to Novel Therapeutics

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Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice