Analysis of Expressed Sequence Tags in Porcine Uterus Tissue

Chai, Hui; Yu, Dongliang; Zhang, Bing; Fu, Yan; Hu, Songnian

doi:10.1007/s10528-008-9211-5

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…40 ALP, also called secretory leukocyte protease inhibitor (SLPI), is a mucosal secreted protein with a low molecular-weight, which inhibits the activities of the neutral serine lysosomal proteinases, elastase and cathepsin-G, contributing to local protection against epithelial cell destruction. 41 Potential regulators of pig endometrial SLPI gene expression include the steroid hormones, estrogen (E) and progesterone (P4). 42 Interestingly, E and P4 regulation of SLPI synthesis appears to be specific to the endometrium.…”

Section: Resultsmentioning

confidence: 99%

Proteomic characterization of the qualitative and quantitative differences in cervical mucus composition during the menstrual cycle

et al. 2015

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The chemical composition of the cervical mucus (CM), its physical characteristics and the volume of secretion change cyclically throughout the menstrual cycle. The aim of this study was to identify the constitutive protein composition of CM of fertile women and the changes in the CM proteome throughout the menstrual cycle. Five fertile women who had a term delivery within 1 year before the study were enrolled. Proteomic analysis was performed using an Ultimate 3000 Nano/Micro-HPLC apparatus equipped with an FLM-3000-Flow manager module and coupled with an LTQ Orbitrap XL hybrid mass spectrometer; bioinformatic software was used for functional and quantitative analysis. 59, 81 and 43 proteins (mean) were respectively identified in the pre-ovulatory, ovulatory and post-ovulatory samples. 38 common proteins were identified. 42, 38 and 17 exclusive proteins were respectively identified in pre-ovulatory, ovulatory and post-ovulatory CM. The main part of CM constituents has a catalytic activity, which is mainly related to hydrolase activity. The label-free quantitative analysis of the common proteins revealed a significant reduction in the protein abundance index for antileukoproteinase, after the ovulation, and a peak of haptoglobin at ovulation. This is the first application of high-resolution MS-based proteomics for the identification of protein constituents of CM. This approach may contribute to the identification of putative biomarkers of the female reproductive tract.

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Section: Resultsmentioning

confidence: 99%

Proteomic characterization of the qualitative and quantitative differences in cervical mucus composition during the menstrual cycle

et al. 2015

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Expression of tissue inhibitor of metalloproteinases-3 messenger RNA and protein in porcine endometrium during implantation

Ren

Guan

et al. 2010

Mol Biol Rep

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Recent evidence points to a stromal decidualization-like response in the pregnant porcine uterus. The objective of this study was to evaluate expression of tissue inhibitors of metalloproteinase-3 (TIMP-3), a sensitive indicator of endometrial stromal decidualization, in endometrium of pregnant sows and to further investigate this phenomenon. Real-time PCR, Western blot and immunostaining analysis were used to study TIMP-3 expression between/at attachment sites of endometrium of Days 13, 18 and 24 pregnant sows. The results indicate that TIMP-3 protein expression was lowest by Day 13 compared with Day 18 (P<0.01) and 24 (P<0.01), and the expression was higher at attachment sites than between attachment sites on Day 13 (P<0.01) and 18 (P<0.01). TIMP-3 intensive immunostaining was observed in stroma of endometrium on Days 13, 18 and 24, and the staining at attachment sites was stronger than between attachment sites. Collectively, these results suggest the crucial role of TIMP-3 in successful implantation and embryo survival and indicate the endometrial stromal decidualization-like in pigs.

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Analysis of Expressed Sequence Tags in Porcine Uterus Tissue

Cited by 2 publications

References 10 publications

Proteomic characterization of the qualitative and quantitative differences in cervical mucus composition during the menstrual cycle

Proteomic characterization of the qualitative and quantitative differences in cervical mucus composition during the menstrual cycle

Expression of tissue inhibitor of metalloproteinases-3 messenger RNA and protein in porcine endometrium during implantation

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