Analysis of expression of TNF-alpha and TGF-beta3 in intrinsic ureteropelvic junction obstruction

Abstract

Abstract:Objectives: Ureteropelvic junction (UPJ) obstruction is of critical importance to understand the histopathology of UPJ obstruction in terms of therapy planning and follow-up. For this purpose, our study was conducted with TNF-α and TGF-β markers to investigate possible underlying problems in intrinsic UPJ obstruction. Methods: Of the patients who had undergone surgery in our clinic, 36 UPJ segments of patients who had undergone dismembered pyeloplasty surgery due to UPJ obstruction and 14 UPJ segments of the patients who had undergone nephrectomy were collected to form 2 groups. All histological sections were examined by applying immunohistochemical transforming growth factor beta 3 (TGF-β3) and tumour necrosis factor alpha (TNF-α) monoclonal antibody dyes. Results: The mean staining values for TNF-α in mucosal tissue and mucosa were 0.53±0.84 and 0.58±0.84, respectively in the obstruction group, whereas the values observed in the control group were 0.86±0.36 and 0.93±0.47, respectively. While the mean staining values in the obstruction group in mucosal tissue and mucosa for TGF-β3 were 1.75±0.73 and 2.17±0.77, respectively, the values established in the control group were 1.14±0.66 and 1.43±0.93, respectively. The difference between the obstruction and control groups were statistically signifi cant for both values (p<0.05). Conclusion: Only a limited number of studies have been carried out on this particular issue. Data from the present study indicate that TGF-β3 and TNF-α may play a role in the histopathogenesis of UPJ obstruction (Tab. 1, Fig. 1

Pilot Study on the Molecular Pathogenesis of Pyeloureteral Junction Obstruction: Underdevelopment or Fibrosis?

“…However, it is worth mentioning that out of these studies, we could only identify one when polymerase chain reaction (PCR) analysis was applied in human ureters and showed that the amount of TGFβ1 mRNA in stenotic tissue was higher than in controls [18]. Another study found that immunohistochemical staining of TGFβ3 in obstructed tissue was higher than in the control tissue [19]. Even though the results of our study of TGFβ in patients with PUJO could not replicate these findings, this could be due to the observations that TGFβ1 is often expressed in the early stages of diseases, especially when macrophage activity is high, as well as when inflammation is present.…”

Predictors of Recoverability of Renal Function after Pyeloplasty in Adults with Ureteropelvic Junction Obstruction

“…A PCN catheter should remain inserted for about 4-12 weeks [1], but patients with PCN drainage are at increased risk of further renal function loss and infection [4,11]. Transforming growth factor-β and urinary tubular enzymes have also been used to predict the recoverability of renal function after the relief from obstruction, though the efficacy and feasibility of these biological agents need further evaluation [14,15]. Renal biopsy may cause infection and bleeding, and given that the histopathological changes in the kidneys are often heterogeneous, the biopsy sample may not reflect the true extent of the effects of UPJO [11].…”