Analysis of F2-isoprostanes in plasma of pregnant women by HPLC-MS/MS using a column packed with core-shell particles

LaRose, Jessica Gokee; Julien, Pierre; Bilodeau, Jean‐François

doi:10.1194/jlr.d034553

Cited by 27 publications

(25 citation statements)

References 34 publications

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“…Twenty mL of blood were collected in EDTA-tubes within 4 hours before the active phase of labor and was centrifuged at 180 × g for 10 minutes as described previously [47]. The resulting plasma was recentrifuged at 1300 × g for 25 min to remove platelets and, was frozen and kept at −80°C until analyzed.…”

Section: Blood Collection and Processingmentioning

confidence: 99%

“…Method I (CHUL cohort): The HPLC-MS/MS analysis of seven F 2 -isoPs was described and fully validated by us recently [47]. Briefly, the chromatography was carried out using a Shimadzu Prominence system (Columbia, MD, USA) coupled to a 3200 QTRAP ® LC/MS/MS system from AB Sciex (Concord, ON, Canada) operated in the negative mode.…”

Section: F 2 -Isops Analysis By Hplc-ms/msmentioning

confidence: 99%

“…The injection volume was 40 μL. The separation was done using a gradient of three solvents (water, acetonitrile and methanol, each containing 0.01 % (v/v) acetic acid) at a flow rate of 0.45 mL/min as described before [47].…”

Section: F 2 -Isops Analysis By Hplc-ms/msmentioning

confidence: 99%

“…The total F 2 -isoPs (free + esterified) were extracted from plasma after alkaline hydrolysis using a liquid-liquid extraction described in details before for method I and II [47]. Method I (CHUL cohort): The HPLC-MS/MS analysis of seven F 2 -isoPs was described and fully validated by us recently [47].…”

Section: F 2 -Isops Analysis By Hplc-ms/msmentioning

confidence: 99%

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F2-isoprostanes are correlated with trans fatty acids in the plasma of pregnant women

LaRose¹,

Julien

Fraser

et al. 2014

Prostaglandins, Leukotrienes and Essential Fatty Acids

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We hypothesized that the mild physiological oxidative stress present during pregnancy could increase both, plasma F 2 -isoprostanes (F 2 -isoPs) by lipid oxidation and trans fatty acids (TFA) through cis-trans isomerization respectively. Plasma samples collected at 12-18 weeks (MIROS cohort; n=65) and 38-41 weeks of pregnancy (CHUL cohort; n = 21) were subjected to alkaline hydrolysis followed by liquid-liquid extraction in order to extract total F 2 -isoPs for quantification by HPLC-MS/MS. Several positive correlations were found between F 2 -isoPs and TFA, measured by GC-FID in plasma phospholipids, such as 6t-18:1, 9t-18:1 and 9t,12c-18:2 (r > 0.306; p < 0.045). Despite its low level, the 9t,12c-18:2 trans isomer, known to be associated to cardiovascular diseases, showed the most significant correlations with F 2 -isoPs. No correlation was observed between F 2 -isoPs and 9t-16:1 or 11t-18:1. In summary, this study suggests either a concomitant phenomenon or a competition between lipid peroxidation and cis-trans isomerisation of the cis precursor fatty acid in vivo during pregnancy.

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Section: Blood Collection and Processingmentioning

confidence: 99%

Section: F 2 -Isops Analysis By Hplc-ms/msmentioning

confidence: 99%

Section: F 2 -Isops Analysis By Hplc-ms/msmentioning

confidence: 99%

Section: F 2 -Isops Analysis By Hplc-ms/msmentioning

confidence: 99%

See 2 more Smart Citations

F2-isoprostanes are correlated with trans fatty acids in the plasma of pregnant women

LaRose¹,

Julien

Fraser

et al. 2014

Prostaglandins, Leukotrienes and Essential Fatty Acids

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“…The other 63 F 2 -IsoP isomers are much less (or for most, not at all) investigated in pregnancy. Indeed, we pioneered the detection of the abundant class VI isomers and reported the first basal values in uncomplicated pregnancies by HPLC-MS/MS [2,10].…”

Section: Introductionmentioning

confidence: 99%

Plasma F2-isoprostane class VI isomers at 12–18 weeks of pregnancy are associated with later occurrence of preeclampsia

Bilodeau

Wei

LaRose³

et al. 2015

Free Radical Biology and Medicine

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Preeclampsia (PE) has long been associated with early oxidative stress whereas the symptoms occur later in pregnancy. We have hypothesized that the oxidative stress in PE, as characterized by the presence of F 2 -isoprostanes (F 2 -isoPs) isomers in late pregnancy, should already be present in plasma at the first regular visit of the obstetrical follow-up. There are 64 possible isomers of F 2 -isoPs derived from the oxidation of arachidonic acid (AA) but only one of these isomers has been investigated so far in PE, the classical 8-iso-PGF 2α . Here, we have investigated two regioisomers of class III (8-iso-15(R)-PGF 2α and 8-iso-PGF 2α ) and a mix of two isomers of class VI (±)5-iPF 2α -VI in plasma samples collected prospectively at 12-18 weeks from normotensive controls (n=60) and pregnant mothers who developed PE later in pregnancy (n=33). The plasma samples were subjected to alkaline hydrolysis followed by liquid-liquid extraction to extract total F 2 -isoPs for later quantification by HPLC-MS/MS. The F 2 -isoPs were normalized to either plasma volume or polyunsaturated fatty acids (PUFAs) levels measured by GC-FID in plasma phospholipids. Early in pregnancy, only the class VI F 2 -isoPs isomers were found in concentrations significantly higher in women developing PE later in pregnancy (+ 13%; p=0.0074). Normalisation of F 2 -isoPs to their substrate, AA, or the omega-3 to omega-6 ratio improved the predictability of PE as determined by receiver operating characteristic (from area under the curve of 0.67 to 0.68 and 0.70 respectively). Interestingly, omega-3 fatty acids were 25% higher in the control group than the PE group (P = 0.0225). Total omega-6 PUFAs correlated to F 2 -Isop isomers only in cases of PE (r >

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LR12‐peptide quantitation in whole blood by RP‐HPLC and intrinsic fluorescence detection: Validation and pharmacokinetic study

Parent

Boudier

Maincent

et al. 2017

Biomedical Chromatography

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A simple, sensitive, selective and robust HPLC method based on intrinsic fluorescence detection was developed for the quantitation of a dodecapeptide (designated as LR12), inhibitor of Triggering Receptor Expressed on Myeloid cells-1, in rat whole blood. Sample treatment was optimized using protein precipitation and solid-phase extraction. Chromatographic separation was carried out in a gradient mode using a core-shell C column (150 × 4.6 mm, 3.6 μm) with mobile phases of acetonitrile and water containing trifluoroacetic acid at 1.0 mL/min. The method was validated using methodology described by the US Food and Drug Administration guidelines for bioanalytical methods. Linearity was demonstrated within the 50-500 ng/mL range and the lower limit of quantitation was 50 ng/mL. Finally, a preliminary pharmacokinetic study after intraperitoneal injection of LR12 in rats was conducted to evaluate both LR12 monomer and its corresponding disulfide dimer, the main product of degradation. Beyond the fact that this paper describes the first fully validated method for LR12 analysis in blood samples, the approach followed here to optimize pre-analytical steps could be beneficial to develop HPLC and/or MS methods for other pharmaceutical peptides.

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Analysis of F2-isoprostanes in plasma of pregnant women by HPLC-MS/MS using a column packed with core-shell particles

Cited by 27 publications

References 34 publications

F2-isoprostanes are correlated with trans fatty acids in the plasma of pregnant women

F2-isoprostanes are correlated with trans fatty acids in the plasma of pregnant women

Plasma F2-isoprostane class VI isomers at 12–18 weeks of pregnancy are associated with later occurrence of preeclampsia

LR12‐peptide quantitation in whole blood by RP‐HPLC and intrinsic fluorescence detection: Validation and pharmacokinetic study

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