The subject of the research is focused on the application of agri-environmental management practices in the agricultural policies of the Republic of Serbia. The research, in the first part, addresses the current attitudes of the policymakers in the sector for agricultural policy, sector for rural development, and the department for the IPARD of the Ministry of Agriculture, Forestry and Water Management, regarding the introduction of agri-ecological measures. In the second part, the current attitudes of the agricultural producers and agricultural advisory employees regarding the main economic and structural factors, as determinants of the adoption of agri-environmental management practices, are analyzed. The findings show that the policymakers have put in place an adequate framework, providing the training and education for the introduction of future AE measures. The findings show that the agricultural producers and the agricultural advisory employees consider agri-ecological measures to be a good way to improve the state of the environment and promote the diversity of nature and organisms. The producers have shown a high willingness to adopt the agri-ecological measures. For them, the highest motivation for using the agri-ecological measures is agriculture-oriented training and education, while the potential obstacles are the complexity of measures, implementation of measures, property rights, purpose of land use, and full-time farm workers. The farmers that perceived that the agri-ecological measures treat all farmers equally contributed the most to predicting the willingness of the agricultural producers to adopt agri-ecological measures. On the other hand, the agricultural advisory service employees perceived as the highest motivations the responsibility of farmers towards future generations, previous experience in the application of similar measures, and agriculture-oriented training and education, while the size of agricultural holdings and the property rights were seen as the largest obstacles. The proposed research on the agri-environmental management practices is particularly relevant in the context of discussions on the reform of the Serbian agricultural policy in light of the EU accession process. The findings of the research shall directly contribute to raising knowledge on the agri-environmental management practices in the Republic of Serbia.