Analysis of forensic genetic parameters of 22 autosomal STR markers (PowerPlex® Fusion System) in a population sample from Bosnia and Herzegovina

Pilav, Amela; Pojskić, Naris; Kalajdžić, Abdurahim; Ahatović, Anesa; Džehverović, Mirela; Čakar, Jasmina

doi:10.1080/03014460.2020.1740319

Cited by 9 publications

(6 citation statements)

References 43 publications

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“…Our study was prepared according to the latest guidelines on publishing forensic and population genetics data for Y-STRs (Carracedo et al, 2014), which state that a minimum of 17 Y-STR loci and 200 samples should be used. Autosomal STR data have already been updated in previous years, firstly by publishing data on 1000 samples analysed for 15 STR loci contained in the PowerPlex 16 system (Promega Corporation;Pilav et al, 2017), followed by publishing data on 22 STR loci contained in the PowerPlex Fusion system (Promega Corporation) on a sample of 600 Bosnian-Herzegovinians (Pilav et al, 2020). The need for an increased sample size and improved data resolution for Y-chromosome studies is addressed in the present study.…”

Section: Resultsmentioning

confidence: 98%

Contemporary population genetics data for 23 Y-STR loci in the general Bosnian-Herzegovinian population

Jordamović

Kojović

Doğan

et al. 2021

GenApp

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Bosnia and Herzegovina is located in the South-Eastern Europe, characterized by numerous historical influences, massive migration processes and complex population structure. For that reason, the aim of this study is to provide an accurate and precise update of the population genetics data of allele frequencies on 23 Y-STR loci in Bosnia and Herzegovina using larger sample size. For this purpose, 480 adult male individuals from the general population have been genotyped over 23 Y-STR loci contained in the PowerPlex Y23 system. Population genetics parameters have been calculated, namely allele and haplotype frequencies, gene and haplotype diversity, as well as Rst and P values for the assessment of interpopulation differences. The obtained results are in close agreement with previously published data for Bosnian-Herzegovinian population, as well as for local subpopulations. This study offers significantly increased resolution and information content, with 454 unique haplotypes. Population comparison reveals no statistically significant differences between the study population and 12 European populations used for comparison, as visualized through an MDS plot and neighbour-joining phylogenetic tree. This study offers representative data for local Y chromosomes that can be used for forensic applications, paternity and kinship testing, as well as for genealogical studies.

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Section: Resultsmentioning

confidence: 98%

Contemporary population genetics data for 23 Y-STR loci in the general Bosnian-Herzegovinian population

Jordamović

Kojović

Doğan

et al. 2021

GenApp

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“…Considering all aforementioned, knowledge about mutation rates and possible mutational processes of different STR loci is important for accurate genetic profiles interpretation (Qian et al, 2015;Hamester et al, 2019). As previously mentioned in the Introduction section, a number of population-genetic studies, focused on evaluating of forensic efficiency parameters of STR markers (Grskovic et al, 2013 2016; Crnjac et al, 2017;Veselinovic et al, 2018;Al-Eitan et al, 2019;Al-Eitan et al, 2020;Pilav et al, 2020;Takic Miladinov et al, 2020) indicated a high degree of reliability of these markers important for all kinds of forensic-genetic analyses, including kinship and parentage testing. Finally, results from these two cases clearly demonstrate that X-STR markers have the potential to solve parentage cases not easily solved by standard analysis based on autosomal STR markers.…”

Section: Discussionmentioning

confidence: 99%

“…Besides high heterozygosity, discriminating power, clearly defined repetitive units and precisely determined allelic variants, simple amplification and detection of STR markers make them ideal for forensic analyses (Khalil et al, 2008;Primorac et al, 2014;Gomes et al, 2020). STR markers located on the autosomal, X and Y chromosomes are highly informative, practical and reliable for all kinds of forensic-genetic analyses including parentage testing, what was strongly supported by many population-genetic studies focused on investigating allele frequencies and forensic parameters (such as heterozygosity, power of discrimination, power of exclusion, polymorphic information content, matching probability and typical paternity index) of autosomal STR (Al-Eitan and Tubaishat, 2016;Al-Eitan et al, 2019;Al-Eitan et al, 2020;Pilav et al, 2020;Takic Miladinov et al, 2020) and X-STR loci (Grskovic et al, 2013;Crnjac et al, 2017;Veselinovic et al, 2018) in different populations. Generally, parentage testing follows Mendelian inheritance law, according to which child receives one allele from the mother and the other allele from the father .…”

Section: Introductionmentioning

confidence: 97%

Untitled

2022

JJBS

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Instructions to AuthorsScopes Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscript that meets the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately one to two months after acceptance.

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“…Developing and validating STR markers was an essential step toward their use in genotyping. The validated STR markers were used to estimate their frequency in various populations worldwide [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Leukemia on the Detection of Short Tandem Repeat (STR) Markers

Alharbi¹,

Alamri²,

El-Shehawi³

2022

Cureus

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Introduction: Short tandem repeats (STRs) have been used for various identity typing methods worldwide. They have high discrimination power in human identification in forensics, paternity testing, missed personal identification, genetic diseases, and gene regulatory functions. They have also been used to detect and monitor the stability of diseases, including various types of cancer. This study aimed to investigate the impact of leukemia on the detection and stability of STR markers.Methods: DNA was isolated from 30 participants (15 with chronic myeloid leukemia( CML) and 15 healthy controls) and used to amplify STR markers using specific primers.Results: We found that the blood of those with leukemia had more 9.3 and 9 alleles at the tyrosine hydroxylase 1 (TH01) marker than the blood of the healthy control samples. The results of this study will help researchers understand leukemia's effect on the detection and stability of STR markers in leukemic patients compared to healthy individuals. Conclusion:Our results demonstrate that STR markers could become useful in genetic studies of leukemia cases.

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Analysis of forensic genetic parameters of 22 autosomal STR markers (PowerPlex® Fusion System) in a population sample from Bosnia and Herzegovina

Cited by 9 publications

References 43 publications

Contemporary population genetics data for 23 Y-STR loci in the general Bosnian-Herzegovinian population

Contemporary population genetics data for 23 Y-STR loci in the general Bosnian-Herzegovinian population

Untitled

The Impact of Leukemia on the Detection of Short Tandem Repeat (STR) Markers

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