Analysis of free and bound NADPH in aqueous extract of human placenta used as wound healer

De, Debashree; Chakraborty, Piyali Datta; Bhattacharyya, Debasish

doi:10.1016/j.jchromb.2009.05.018

Cited by 20 publications

(24 citation statements)

References 34 publications

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“…Earlier spectral shift in placenta extract has also been observed in case of NADPH quantitation [13]. The spectral shift is a common phenomenon for the binding of fluorephore in with the specific molecules in a multi-component system [13] [14]. It has been observed that the extract without addition of EtBr has some background fluorescence in the said spectral zone.…”

Section: Discussionmentioning

confidence: 80%

“…Characterization of an aqueous extract of human placenta which is used as a licensed drug, leads to the identification of several bioactive components like NADPH [13] [14]; fibronectin type III-like peptide has been shown to stabilize trypsin activity [4] [15] and a ubiquitin-like peptide which exhibits collagenolytic activity and is probably involved in the remodelling phase of healing [16]. The extract also exhibits anti-microbial activity against commonly occurring pathological organisms [17], in vitro induction of NO by mouse peritoneal macrophages [18] along with enhancement of cell adhesion [19].…”

Section: Introductionmentioning

confidence: 99%

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Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Chakraborty¹,

Goswami²,

Bera³

et al. 2014

AJAC

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Characterization of an aqueous extract of human placenta, used as a licensed drug for wound healing, leads to the identification of several bioactive components including polydeoxyribonucleotides (PDRNs). PDRNs are mixture of DNA fragments of different molecular weight. A spectrofluorimetric method of quantitation of PDRNs in the aqueous extract of human placenta by using ethidium bromide (EtBr) has been described here. It has been demonstrated by thin layer chromatography (TLC) followed by reversed phase HPLC that EtBr binds specifically with the PDRN fraction of the multi-component extract. The binding specificity of EtBr has been verified by the analysis of emission spectra of the extract. A concentration of 0.29 µg/ml EtBr exhibits a linear range of standard CT-DNA from 0.5 -5 µg/ml of buffer (R 2 = 0.992). The same concentration of EtBr shows a linear range of measurements of placenta extract from 5 -35 µl/ml of buffer (R 2 = 0.976). The points of the curve were the average of three sets where maximum variation observed was ±3%. PDRN content of the extract has been estimated based on the resultant fluorescence emission (after background correction) with respect to the standard calibration curve of calf thymus DNA (CT-DNA). Estimation of PDRN in a large number of batches of placenta extract (n = 100) has been done. The statistical analysis of the estimation was found to be significant and the lower and upper levels of PDRN were 158.30 and 239.03 µg/ml of the extract respectively. This easy-to-use method of estimation of PDRN in multi-component biological extract is reported for the first time. This will help in quantitation of PDRNs for other biological extracts.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Chakraborty¹,

Goswami²,

Bera³

et al. 2014

AJAC

Self Cite

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“…Aqueous extract from placenta is used as a licensed drug for wound healing under different trade names in India and overseas countries (Datta and Bhattacharyya, 2004a). This could be due the fact that the aqueous extract is a rich source of various bioactive peptides with tissue regeneration potential (Chakraborty and Bhattacharyya, 2005a;De et al, 2009). In addition, the extract also retains amino acids, nucleotides, polydeoxyribonucleotides and carbohydrates that might be responsible for wound healing.…”

Section: Role Of Placental Extract In Wound Healingmentioning

confidence: 99%

Aqueous Extract of Human Placenta

Chakraborty¹,

Bhattacharyya²

2012

Recent Advances in Research on the Human Placenta

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“…The wound healing potency of an aqueous human placental extract (HPE) has been clinically established in previous studies (1,2). HPE has numerous applications in the treatment of chronic soft tissue ulcers and in the stimulation of endogenous growth factors, while it possesses anti-inflammatory and antimicrobial properties (1,2).…”

Section: Introductionmentioning

confidence: 99%

“…HPE has numerous applications in the treatment of chronic soft tissue ulcers and in the stimulation of endogenous growth factors, while it possesses anti-inflammatory and antimicrobial properties (1,2). HPE efficiently activates adenosine A2A receptors and is not degraded in vivo, meaning it is more efficient than other types of therapy, including vacuum sealing drainage, local application of growth factor, and traditional Chinese medicine (3).…”

Section: Introductionmentioning

confidence: 99%

Preparation of a novel composite nanofiber gel-encapsulated human placental extract through layer-by-layer self-assembly

Liu

Chen

Zhou

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Aqueous human placenta extract (HPE) has been previously used to treat chronic soft tissue ulcer; however, the optimal dosage of HPE has yet to be elucidated. The present study investigated a novel nanofiber gel composed through layer-by-layer (LbL) self-assembly, in which HPE was encapsulated. IKVAV, RGD, RAD16 and FGL-PA were screened and combined to produce an optimal vehicle nanofiber gel through LbL assembly. Subsequently, the aqueous HPE was encapsulated into this nanofiber at the appropriate concentration, and the morphology, particle size, drug loading efficacy, encapsulation rate, release efficiency and structure validation were detected. The encapsulation efficiency of all three HPE samples was >90%, the nanofiber gel exhibited a slow releasing profile, and the structure of HPE encapsulated in the nanofiber gel was unvaried. In conclusion, this type of novel composite nanocapsules may offer a promising delivery system for HPE.

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Analysis of free and bound NADPH in aqueous extract of human placenta used as wound healer

Cited by 20 publications

References 34 publications

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

Aqueous Extract of Human Placenta

Preparation of a novel composite nanofiber gel-encapsulated human placental extract through layer-by-layer self-assembly

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