Analysis of Fusarium ear rot and fumonisin contamination in testcrosses of a maize biparental population

Galić, Vlatko; Šimić, Domagoj; Franić, Mario; Brkić, Andrija; Jambrović, Antun; Brkić, Josip; Ledenčan, Tatjana

doi:10.1590/1984-70332019v19n1a06

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…Similarly, Cao et al (2022) found strong genotypic correlation (r g = 0.85) between FUM and FER severity after kernel inoculation. Based on this, selection for FER-resistant lines would indirectly reduce fumonisins accumulation (Maschietto et al, 2017;Galićet al, 2019). However, our analysis also revealed the existence of one specific QTL for FUM (qFER12, PVE = 8%) on chromosome 5 (Figure 2) and one for DON (qGER12, PVE = 15%) on chromosome 9 (Figure 3), which were identified as independent MQTL for these traits.…”

Section: Figuresupporting

confidence: 55%

Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize

Akohoue

Miedaner

2022

Front. Plant Sci.

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Fusarium (FER) and Gibberella ear rots (GER) are the two most devastating diseases of maize (Zea mays L.) which reduce yield and affect grain quality worldwide, especially by contamination with mycotoxins. Genetic improvement of host resistance to effectively tackle FER and GER diseases requires the identification of stable quantitative trait loci (QTL) to facilitate the application of genomics-assisted breeding for improving selection efficiency in breeding programs. We applied improved meta-analysis algorithms to re-analyze 224 QTL identified in 15 studies based on dense genome-wide single nucleotide polymorphisms (SNP) in order to identify meta-QTL (MQTL) and colocalized genomic loci for fumonisin (FUM) and deoxynivalenol (DON) accumulation, silk (SR) and kernel (KR) resistances of both FER and GER, kernel dry-down rate (KDD) and husk coverage (HC). A high-resolution genetic consensus map with 36,243 loci was constructed and enabled the projection of 164 of the 224 collected QTL. Candidate genes (CG) mining was performed within the most refined MQTL, and identified CG were cross-validated using publicly available transcriptomic data of maize under Fusarium graminearum infection. The meta-analysis revealed 40 MQTL, of which 29 were associated each with 2-5 FER- and/or GER-related traits. Twenty-eight of the 40 MQTL were common to both FER and GER resistances and 19 MQTL were common to silk and kernel resistances. Fourteen most refined MQTL on chromosomes 1, 2, 3, 4, 7 and 9 harbored a total of 2,272 CG. Cross-validation identified 59 of these CG as responsive to FER and/or GER diseases. MQTL ZmMQTL2.2, ZmMQTL9.2 and ZmMQTL9.4 harbored promising resistance genes, of which GRMZM2G011151 and GRMZM2G093092 were specific to the resistant line for both diseases and encoded “terpene synthase21 (tps21)” and “flavonoid O-methyltransferase2 (fomt2)”, respectively. Our findings revealed stable refined MQTL harboring promising candidate genes for use in breeding programs for improving FER and GER resistances with reduced mycotoxin accumulation. These candidate genes can be transferred into elite cultivars by integrating refined MQTL into genomics-assisted backcross breeding strategies.

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Section: Figuresupporting

confidence: 55%

Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize

Akohoue

Miedaner

2022

Front. Plant Sci.

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“…Although the genotypic variances were quite similar for both landraces, PE showed higher genotype × environment variance resulting in a slightly higher H 2 for KE (Table 1). A large effect of the environmental conditions on ear rot resistance has been reported several times in the literature (Giomi et al 2016;Han et al 2018;Galić et al 2019;Morales et al 2019). Therefore, it is important to phenotype lines for GER resistance in multi-environmental trials.…”

Section: Variation For Ger Severity and Agronomic Traits In European mentioning

confidence: 99%

Exploiting genetic diversity in two European maize landraces for improving Gibberella ear rot resistance using genomic tools

et al. 2020

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Key message High genetic variation in two European maize landraces can be harnessed to improve Gibberella ear rot resistance by integrated genomic tools. Abstract Fusarium graminearum (Fg) causes Gibberella ear rot (GER) in maize leading to yield reduction and contamination of grains with several mycotoxins. This study aimed to elucidate the molecular basis of GER resistance among 500 doubled haploid lines derived from two European maize landraces, “Kemater Landmais Gelb” (KE) and “Petkuser Ferdinand Rot” (PE). The two landraces were analyzed individually using genome-wide association studies and genomic selection (GS). The lines were genotyped with a 600-k maize array and phenotyped for GER severity, days to silking, plant height, and seed-set in four environments using artificial infection with a highly aggressive Fg isolate. High genotypic variances and broad-sense heritabilities were found for all traits. Genotype-environment interaction was important throughout. The phenotypic (r) and genotypic ($${r}_{g}$$ r g ) correlations between GER severity and three agronomic traits were low (r = − 0.27 to 0.20; $${r}_{g}\hspace{0.17em}$$ r g = − 0.32 to 0.22). For GER severity, eight QTLs were detected in KE jointly explaining 34% of the genetic variance. In PE, no significant QTLs for GER severity were detected. No common QTLs were found between GER severity and the three agronomic traits. The mean prediction accuracies ($$\rho $$ ρ ) of weighted GS (wRR-BLUP) were higher than $$\rho $$ ρ of marker-assisted selection (MAS) and unweighted GS (RR-BLUP) for GER severity. Using KE as the training set and PE as the validation set resulted in very low $$\rho $$ ρ that could be improved by using fixed marker effects in the GS model.

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“…Besides this, the GRMZM2G060216 gene refers to the transcription factor LG2, which is related to systemic acquired resistance by the salicylic acid-mediated signaling pathway (Chen et al 2012). Galić et al (2019) in a study to assess the factors affecting Fusarium ear rot and fumonisin contamination of maize, identi ed genes that can confer resistance, and found genes that code for NAC transcription factor.…”

Section: Discussionmentioning

confidence: 99%

A Genome-Wide Association Study for Fumonisin Contamination Resistance in Tropical Maize

Silva

Guimarães

Tinoco

et al. 2021

Preprint

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Native genetic resistance has been considered an effective and environmentally safe alternative to control fungal infections and to reduce mycotoxins accumulation in maize grains. Marker-assisted breeding can be used to accelerate the genetic gains for fumonisin contamination resistance. Thus, the objective of this study was to identify quantitative trait loci (QTLs) and candidate genes for resistance to fumonisin contamination in an Embrapa’s tropical maize panel. Two-hundred and five inbreed lines were evaluated in three field trials Brazil in order to quantify fumonisin contamination in maize grains. The lines were genotyped-by-sequencing (GBS), generating 385,654 high-quality polymorphic SNPs. Mycotoxins in the grain samples were isolated using commercial immunoaffinity columns and its concentrations were evaluated by fluorometric technique. Nine quantitative trait loci (QTL) were found associated with fumonisin concentration resistance in maize. Seven candidate genes with annotated functions associated with biosynthetic pathways of pathogen resistance and four genes have not been previously described as related to fumonisins contamination resistance. These findings will be important to better understand the fumonisin contamination resistance and to support the development of SNP markers to accelerate the selection process in tropical maize breeding programs.

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Analysis of Fusarium ear rot and fumonisin contamination in testcrosses of a maize biparental population

Cited by 8 publications

References 29 publications

Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize

Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize

Exploiting genetic diversity in two European maize landraces for improving Gibberella ear rot resistance using genomic tools

A Genome-Wide Association Study for Fumonisin Contamination Resistance in Tropical Maize

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