Analysis of gene expression associated with cold acclimation in blueberry floral buds using expressed sequence tags

Dhanaraj, Anik L.; Slovin, Janet P.; Rowland, Lisa J.

doi:10.1016/j.plantsci.2003.11.013

Cited by 80 publications

(80 citation statements)

References 53 publications

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“…Ubiquitin ligases, MYB transcription factor and BURP dehydration-responsive gene RD22, for example, are known to be concerned with plant cold resistance (Nagaoka and Takano, 2003;Dhanaraj et al, 2004;Todgham et al, 2007). In this study, one of the ESTs obtained showed high homology with ubiquitin ligase (93%).…”

Section: Discussionmentioning

confidence: 62%

“…This would probably lead to signal cascades and increase the expression of hardiness genes. Previous studies have indicated that under cold stress, Populus increases its expression levels of Ca 2+ or CaM in plants (Dhanaraj et al, 2004). Besides, G protein has GTPase activity and can function as a signal transducer or molecular switch in signal transduction pathways.…”

Section: Discussionmentioning

confidence: 99%

“…In autumn, the content of ABA in buds of some perennial woody plants is relatively high and the moisture content is relatively low; in winter, ABA levels tends to decrease. Dhanaraj et al (2004) suggested that the expression level of RD22 in blueberry cDNA library was influenced indirectly by the content of ABA or water in buds when under cold stress. According to RT-PCR, the occurrence of RD22 in the Heilongjiang Seedling cDNA library was a response to cold stress.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is necessary to clone the genomes of plant into cDNA libraries. By differential screening of cDNA libraries, several genes related to low temperature have been identified and characterized in various plant species, such as blueberry (Dhanaraj et al, 2004), Hevea brasiliensis (Cheng et al, 2008), and aspen (Maestrini et al, 2009). In grape, da Silva et al (2005) constructed several cDNA libraries with different varieties and tissues of European wine-making grapes to investigate gene expression profiles in response to various forms of stress.…”

Section: Introductionmentioning

confidence: 99%

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Construction of a cDNA library of the Chinese wild Vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags

Zhang¹,

Liu²,

Rui³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. A cDNA library of Chinese wild Vitis amurensis, which is the most cold-resistant species in the genus Vitis, was constructed using young leaves of seedlings of the clone Heilongjiang potted and subjected to cold stress. The leaves were harvested at various times after cold stress for total RNA extraction, which was used to generate expressed sequence tags (ESTs). The titer of the original library was 2.67 x 10 6 pfu/mL and the corresponding combination frequency was 98.5%. The test values of the amplified library were 1.53 x 10 9 pfu/ mL and 98.2%, respectively. After randomly choosing, cloning and sequencing, 227 ESTs with high quality were obtained and submitted to GenBank database. Using BLASTX, 79.7% of the ESTs shared homology with known functional DNA sequences and 20.3% shared significant homology with unknown proteins. Some potential hardinessrelated ESTs were obtained, which were involved in signal transduction, Analysis of cold-responsive genes from Vitis amurensis stress inducement, defense reactions, transcription factors, etc. Some functional genes identified from the cDNA library have potential for plant defense. These sequences will be subjected to further researches on hardiness genes and their molecular mechanisms.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Construction of a cDNA library of the Chinese wild Vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags

Zhang¹,

Liu²,

Rui³

et al. 2013

Genet. Mol. Res.

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“…Surprisingly, the chemical modifications described did not result in significant improvements of DNA quality and quantity in any of the methods tested in comparison to the standard manufacturer's protocols although this was expectable from our previous experiences with this woody ornamental regarding both DNA and RNA isolation. Furthermore, other authors (Dhanaraj et al 2004) already described the requirement of these supplemental chemicals for the extraction of floral bud RNA of Vaccinium, a species of the same plant family (Ericaceae).…”

Section: Resultsmentioning

confidence: 99%

Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)

Borchert¹,

Gawenda²

2010

Electron. J. Biotechnol.

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Calluna vulgaris is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of 'bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient screening of whole segregating populations e.g. for molecular marker and mapping approaches is a requirement. We therefore aimed to introduce the powerful tool of amplified fragment length polymorphisms (AFLP ® ), a widely and successfully applied method, into our methodological assortment. As an essential prerequisite, the isolated DNA should be of adequate quality which is a common obstacle when dealing with woody species and their interfering secondary components/metabolites. The results of screening different and modified DNA isolation protocols are described. As the outcome of our evaluations of reaction conditions during the AFLP ® procedure, we circumstantiate a functional protocol ranging from DNA extraction to visualization of AFLP ® banding patterns for the woody crop C. vulgaris. This method is suitable for high throughput genetic applications and may even be transferable to other species. In addition, costs are reduced by reasonable reagents and multiplexing assays. *Corresponding authorRecently, we reported a DNA isolation method for the ornamental crop C. vulgaris suitable for randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses (Borchert et al. 2008). However, this method incorporates potentially harmful phenol/chloroform extraction steps and requires approximately 14 hrs of working time for 24 samples (from tissue grinding to quantification and qualification). Moreover, RAPD and ISSR techniques are not adequate e.g. regarding reproducibility especially for mapping of traits. Therefore, we aimed at improving our method by simplifying the DNA isolation protocol for C. vulgaris and establishing amplified fragment length polymorphisms (AFLP ® ) methodology. Both methods should comply with high-throughput (htp) requirements in terms of time-and cost-efficiency. AFLPs® require high-quality DNA. The isolation of such DNA of herbaceous and woody plants is commonly hindered due to the accumulation of polyphenolic and polysaccharide compounds that interfere during subsequent processing of the DNA. Several manual protocols based on different assays to eliminate these contaminants (e.g. CTAB, silica; (Rogstad, 2003)) are available for such cases. However, for each species the adaptation of the isolation method is usually required.

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Berry Crops

Hancock

Song

Sink

2008

Compendium of Transgenic Crop Plants

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Considerable genetic improvement has been achieved in the berry crops, mostly through conventional breeding. Strawberry breeders have focused on improving local adaptations, fruit quality, productivity, and disease resistance, and many are interested in developing day‐neutral cultivars. Transgenic strawberries have been produced with herbicide and pest resistances. A number of genes from strawberry have also been characterized that are associated with fruit ripening and maturation, and an effective marker‐free transformation process has been developed. The major objectives of blueberry breeders have centered on high plant vigor, improved disease resistance, flavor, longer fruit storage, and expanded harvest dates. Transgenic blueberries have been produced with herbicide resistance. Cranberry breeders have focused on early maturing fruit, uniform large size, intense color, keeping quality, high productivity, disease resistance, and plant vigor. The Bt gene ( Bacillus thuringiensis ) has been incorporated into cranberry through transformation. Raspberry breeders have been most interested in improving fruit quality and increasing the efficiency of fruit production. The development of primocane fruiting types has also been a high priority in many programs. Transformation was used to develop a red raspberry cultivar with resistance to Raspberry bushy dwarf virus. Blackberry breeders have been interested in increasing the climatic zone, improving harvest efficiency, reducing chilling requirements, improving fruit quality, and generating primocane fruiting types. Little transformation work has been done on blackberry.

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Analysis of gene expression associated with cold acclimation in blueberry floral buds using expressed sequence tags

Cited by 80 publications

References 53 publications

Construction of a cDNA library of the Chinese wild Vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags

Construction of a cDNA library of the Chinese wild Vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags

Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)

Berry Crops

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