Analysis of gene expression by ESTs from suppression subtractive hybridization library in Chenopodium album L. under salt stress

Abstract

To identify genes expression in Chenopodium album exposed to NaCl stress and screen ESTs related to salt stress, a subtractive suppression hybridization (SSH) library of C. album under salt stress was constructed in the present study. Random EST sequencing produced 825 high-quality ESTs with GenBank ID GE746311-GE747007, which had 301 bp of average size and were clustered into 88 contigs and 550 singletons. They were classified into 12 categories according to their function annotations. 635 ESTs (76.97%) showed similarities to gene sequences in the non-redundancy database, while 190 ESTs (23.03%) showed low or no similarities. The transcriptional profiles of 56 ESTs randomly selected from 347 unknown or novel ESTs of SSH library under varying NaCl concentration and at different time points were analyzed. The results indicated that a high proportion of tested ESTs were activated by salt stress. Four in 56 ESTs responded to NaCl were also enhanced in expression level when exposed to ABA and PEG stresses. The above four ESTs were validated by northern blotting which was consistent with the results of RT-PCR. The results suggested that genes corresponded to these ESTs might be involved in stress response or regulation. The complete sequences and detailed function of these ESTs need to be further studied.

Overexpression of a Plasma Membrane-Localized SbSRP-Like Protein Enhances Salinity and Osmotic Stress Tolerance in Transgenic Tobacco

“…This unusual characteristics, alongside other factors such as its oligosaccharides, proteome and metabolites, provides an opportunity to investigate its salt tolerance system (Jha et al, 2012; Joshi et al, 2012; Mishra et al, 2013, 2015; Patel et al, 2016a). Different salt tolerance mechanisms have been reported from halophytes (Jha et al, 2011; Chaturvedi et al, 2014; Singh et al, 2014a; Udawat et al, 2016) and several EST databases have been created for numerous halophytes, including S. brachiata (Jha et al, 2009), Alfalfa (Jin et al, 2010), and Chenopodium album (Gu et al, 2011). …”

Differentially Expressed Long-Term Salinity Responsive Sequences in Halophyte Suaeda maritima (L.) Dumort

“…By using two specific hybridizations and two PCR specific amplifications, SSH has the advantage of decreasing false positive rate, improve the screening efficiency, higher degree of target sequence enrichment and higher consistency (Diatchenko et al, 1996). It has been successfully applied in the identification of salt responsive genes in various plants such as Thellungiella halophila (Wang et al, 2004), Aeluropus littoralis (Zouari et al, 2007), Bruguiera cylindrica (Wong et al, 2007, Chenopodium album (Gu at al., 2011), Mesembryanthemum crystallinum (Roeurn et al, 2016) and Suaeda nudiflora (Jothiramshekar et al, 2020).…”

A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco

“…It can be envisaged that these unknown genes constitute the unique genetic make-up of the plant helping it to sustain itself under stress condition. EST databases of different halophytic plants show a large percentage of unknown genes like Sueda salsa (22%, [17]), Mesembryanthum crystallinum [18], Thellungiella halophilla (32%, [19]), Avicennia marina (30%, [20]), Limonium sinense (37%, [21]), Aleuropus littoralis (20%, [22]), Spartina alterniflora (13%, [23]), Macrotyloma uniflorum (30%, [24]), S. brachiata (29%, [25]), Tamarix hispida (21%, [26]), Alfalfa (22%, [27]) and Chenopodium album (42%, [28]). Previously, we have identified approximately 1000 ESTs in response to salt stress from S. brachiata [25] and also characterized some important abiotic stress tolerant genes ( SbGST , [29]; SbMAPKK , [15]; SbDREB2A , [30]; SbNHX1 , [31]; SbASR1 , [32]; SbSOS1 , [33]; SbSI-1 , [34]).…”