Analysis of Gene Expression in Peripheral Blood Eosinophils from Patients with Atopic Dermatitis by Differential Display

Hashida, Ryoichi; Ogawa, Katsuhiro; Miyagawa, Masami; Sugita, Yuji; Takahashi, Eiki; Nagasu, Takeshi; Katsunuma, Toshio; Akasawa, Akira; Tsujimoto, Gozoh; Matsumoto, Kenji; Saito, Hirohisa

doi:10.1159/000070478

Cited by 10 publications

(8 citation statements)

References 23 publications

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“…These studies have revealed some novel genes implicated in performing essential roles in the pathological conditions characteristic of allergic diseases (Matsumoto et al , 2004aHeishi et al 2002;Imai et al 2002;Hashida et al 2003;Nagata et al 2003;Ogawa et al 2004;Kagaya et al 2005a). A successful example of this approach has been the identification of the nuclear receptor gene family whose enhanced expression in peripheral blood eosinophils correlated with the pathological condition of AD.…”

Section: Comprehensive Gene Expression Analysis In Eosinophils From Pmentioning

confidence: 99%

The NR4A nuclear receptor family in eosinophils

Hashida¹,

Ohkura²,

Saito

et al. 2006

J Hum Genet

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It is well-known that many members of the family of nuclear receptors have been implicated in human diseases, and metabolic disorders in particular. The NR4A nuclear receptor family consists of three members, Nur77, Nurr1, and NOR1. All of these are orphan receptors, and Nur77 and NOR1 exert possible pathological roles in immune diseases through the modulation of leukocyte functions. CD30 stimulation, which induces eosinophil-specific apoptosis, markedly enhances expression of Nur77 and NOR1 in eosinophils. This suggests the possibility of pharmacological modulation of Nur77-or NOR1-specific apoptotic pathways via receptor-dependent transactivation. In this review, we discuss treatment of allergic diseases by low molecular weight compounds acting through the NR4A receptor family to cause eosinophil apoptosis. NR4A nuclear receptor genes were selected following comprehensive analysis of differentially expressed genes in eosinophils of atopic dermatitis patients compared with healthy volunteers.

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Section: Comprehensive Gene Expression Analysis In Eosinophils From Pmentioning

confidence: 99%

The NR4A nuclear receptor family in eosinophils

Hashida¹,

Ohkura²,

Saito

et al. 2006

J Hum Genet

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“…We compared gene expression in peripheral blood eosinophils from both AD patients and healthy volunteers and identifi ed some genes that were differentially expressed [12] . Some of the differentially expressed genes belonged to the human orphan nuclear receptor family, NR4A.…”

Section: Nr4a Orphan Nuclear Receptor Family In Peripheral Blood Eosimentioning

confidence: 99%

NR4A Orphan Nuclear Receptor Family in Peripheral Blood Eosinophils from Patients with Atopic Dermatitis and Apoptotic Eosinophils in vitro

Kagaya¹,

Hashida²,

Ohkura

et al. 2005

Int Arch Allergy Immunol

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To identify novel genes related to the clinical signs of atopic dermatitis (AD), differentially expressed genes were sought in peripheral blood eosinophils from both AD patients and healthy volunteers. RNA was prepared from eosinophils, expression of various genes was monitored using the Affymetrix GeneChip, and expression was quantified by real-time RT-PCR. Two genes, Nur77 and NOR1, members of NR4A orphan nuclear receptor family, were expressed at a significantly higher level in AD patients than in healthy volunteers. Expression of another gene in the NR4A receptor family, Nurr1, was also higher in AD patients than in healthy volunteers. When peripheral blood leukocytes from healthy volunteers were fractionated, NOR1 expression was highest in eosinophils, but expression of Nur77 and Nurr1 genes was not eosinophil-specific. Extremely intense apoptosis was induced in both eosinophils and an eosinophil cell line, AML14.3D10, by treatment with antibody (Ab) to both CD30 and Fas. Rapid expression of the genes for the NR4A receptor family was observed with anti-CD30 Ab treatment but not with anti-Fas Ab. The NR4A orphan nuclear receptor family gene expression and the subsequent eosinophil apoptosis were downregulated by the MAPK inhibitor, U0126. These results suggest that the expression of the NR4A receptor family genes through CD30 signaling may regulate eosinophil apoptosis in allergic conditions such as AD.

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“…The analysis of genes differentially expressed between ad patients and normal individuals and between the different phases of ad may provide information on the molecular pathogenesis of the disease. To identify the genes involved in allergic diseases, we analyzed transcription profiles in peripheral blood immune cells from patients with allergic diseases, and found many genes that were differentially expressed between patients and healthy individuals (1,2). during differential display (dd) gene expression studies on peripheral blood cd3+ T cells from ad patients, we observed that the level of expression of a gene later designated coiledcoil domain containing 132, transcript variant 1 (CCDC132) was markedly higher in ad patients than in normal controls.…”

Section: Introductionmentioning

confidence: 99%