Analysis of gene expression in red maple (Acer rubrum) and trembling aspen (Populus tremuloides) populations from a mining region

Kalubi, K. N.; Michael, P.; Omri, Abdelwahab

doi:10.1007/s13258-018-0670-8

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…Similar studies that we have conducted demonstrated that potassium nitrate can induce a high level of transcription of 1aminocyclopropane-1-carboxylic acid (ACC) deaminase and NAS3 genes in leaves of Quercus rubra seedlings exposed to at low dose (150 mg/kg) of Ni. Our results also confirmed findings pf a field study on P. tremuloides and Acer rubrum indicating that the low level of bioavailable nickel in metal-contaminated soils (< 150 mg / kg) cannot induce differential transcription of NAS3 and NRAMP4 (Kalubi et al, 2018).…”

Section: Discussionsupporting

confidence: 89%

“…Each reaction was run in triplicates using the MJ Research PTC-200 Thermal Cycler. The set program includes (1) initial denaturing at 95°C for 15 min; (2) denaturing at 94°C for 30 sec; (3) 30 sec at 55°C annealing; (4) elongation at 72°C for 30 sec; (5) read (6) repeat step 2-6 for 41 cycles; (7) final elongation at 72°C for 7 min; (8) melting curve 72-95°C, every 1°C, hold for 10 sec; and (9) final elongation at 72°C for 3 min as described in Kalubi et al (2018). The RT-qPCR was performed two separate times per gene target and samples were loaded in triplicates.…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

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High Level of Nicotianamine Synthase (NAS3) and Natural Resistance Associated Macrophage Protein (NRAMP4) Gene Transcription Induced by Potassium Nitrate in Trembling Aspen (Populus tremuloides)

Czajka¹,

Nkongolo²

2018

American Journal of Biochemistry and Biotechnology

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Changes in gene transcription in response to excess metal concentrations have been reported in many organisms, including yeast, microorganisms and plants. Most investigations on the effects of nickel toxicity in plants use commercial salts whose effects have not been analyzed in detail. The main objective of the present study was to determine the effects of different doses of nickel nitrate and potassium nitrates on gene transcription in Populus tremuloides. Four month-old P. tremuloides seedlings were treated with different doses of nitrate salts including 150 mg/kg, 500 mg/kg, 800 mg/kg and 1, 600 mg/kg. A significant increase of Nicotianamine Synthase (NAS3) gene transcription was induced by the 400 mg/kg and 800 mg/kg of nickel nitrate doses compared to water. This upregulation was driven by nitrate rather than nickel. Likewise, the 800 mg/kg and 1,600 mg/kg doses of potassium nitrate resulted in significant increase in the transcription of Natural Resistance Associated Macrophage Protein (NRAMP4) gene compared to water control and the 150 mg/kg dose. This differential transcription of this gene was caused by potassium. Our results also confirmed that the low level of bioavailable nickel in metalcontaminated soils (<150 mg/kg) cannot induce differential transcription of NAS3 and NRAMP4. The use of nitrate without nickel should be required as additional controls in any study assessing effects of Ni using nickel nitrate salts.

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Section: Discussionsupporting

confidence: 89%

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

High Level of Nicotianamine Synthase (NAS3) and Natural Resistance Associated Macrophage Protein (NRAMP4) Gene Transcription Induced by Potassium Nitrate in Trembling Aspen (Populus tremuloides)

Czajka¹,

Nkongolo²

2018

American Journal of Biochemistry and Biotechnology

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“…Nutrient use efficiency and non-specific interactions with soil microorganisms have been revealed as principal mechanisms underlying the establishment of this species in post-mining areas [ 16 , 21 ]. Modifications in plant metabolism are common characteristics of plants growing in minelands [ 22 , 23 ]. However, little is known about the adaptive molecular mechanisms developed by this species throughout its evolutionary history.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms Underlying Mimosa acutistipula Success in Amazonian Rehabilitating Minelands

Nascimento

Herrera

Costa

et al. 2022

IJERPH

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Mimosa acutistipula is endemic to Brazil and grows in ferruginous outcrops (canga) in Serra dos Carajás, eastern Amazon, where one of the largest iron ore deposits in the world is located. Plants that develop in these ecosystems are subject to severe environmental conditions and must have adaptive mechanisms to grow and thrive in cangas. Mimosa acutistipula is a native species used to restore biodiversity in post-mining areas in canga. Understanding the molecular mechanisms involved in the adaptation of M. acutistipula in canga is essential to deduce the ability of native species to adapt to possible stressors in rehabilitating minelands over time. In this study, the root proteomic profiles of M. acutistipula grown in a native canga ecosystem and rehabilitating minelands were compared to identify essential proteins involved in the adaptation of this species in its native environment and that should enable its establishment in rehabilitating minelands. The results showed differentially abundant proteins, where 436 proteins with significant values (p < 0.05) and fold change ≥ 2 were more abundant in canga and 145 in roots from the rehabilitating minelands. Among them, a representative amount and diversity of proteins were related to responses to water deficit, heat, and responses to metal ions. Other identified proteins are involved in biocontrol activity against phytopathogens and symbiosis. This research provides insights into proteins involved in M. acutistipula responses to environmental stimuli, suggesting critical mechanisms to support the establishment of native canga plants in rehabilitating minelands over time.

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Metal Toxicity and Resistance in Plants and Microorganisms in Terrestrial Ecosystems

Narendrula-Kotha

Theriault

Mehes-Smith

et al. 2019

Reviews of Environmental Contamination and Toxicology

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Analysis of gene expression in red maple (Acer rubrum) and trembling aspen (Populus tremuloides) populations from a mining region

Cited by 7 publications

References 44 publications

High Level of Nicotianamine Synthase (NAS3) and Natural Resistance Associated Macrophage Protein (NRAMP4) Gene Transcription Induced by Potassium Nitrate in Trembling Aspen (Populus tremuloides)

High Level of Nicotianamine Synthase (NAS3) and Natural Resistance Associated Macrophage Protein (NRAMP4) Gene Transcription Induced by Potassium Nitrate in Trembling Aspen (Populus tremuloides)

Molecular Mechanisms Underlying Mimosa acutistipula Success in Amazonian Rehabilitating Minelands

Metal Toxicity and Resistance in Plants and Microorganisms in Terrestrial Ecosystems

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