Analysis of gene expression in intracranial aneurysms

Abstract

Background: Different gene expression profiles are observed in intracranial aneurysm tissue. Understanding these genes and what regulates their expression will help us to understand intracranial aneurysm pathogenesis. We investigated whether differences in gene expression in intracranial aneurysms. Methods: Sixteen intracranial aneurysm tissues were compared with 16 matched samples from the superficial temporal artery as controls. We detected the gene expression profiles in these samples with the Human U133 Plus 2.0 GeneChip. Results: A total of 2142 differentially expressed gene transcripts were detected based on the gene expression profile. Verification analysis showed that the VCAM1, MAGI2, PPP2R2B, PPP2R3A genes were associated with the occurrence and development of intracranial aneurysm. These genes mainly encode cell adhesion molecules (CAMs) and ERK/JNK signaling pathways. Conclusion: Changes of genes expression involved in immune and inflammatory reactions, cell adhesion molecules may be associated with the development of aneurysms.

Identification of Hub Genes Associated with the Pathogenesis of Intracranial Aneurysm via Integrated Bioinformatics Analysis

“…The validation analysis by Wang et al showed that VCAM1, MAGI2, PPP2R2, PPP2R3A genes may be related to the occurrence and development of IA, and these genes were mainly involved in encoding cell adhesion molecules (CAMs) and extracellular regulated protein kinase (ERK)/c-JunN-terminal kinase (JHK) signaling pathways. 16 The team of Wei and coworkers found that CD40, CD40LG, NOS1, STRN, MCM4, MYH11 and miR-125b may play an important role in the pathogenesis of IA. 14 In fact, due to the difficulty in obtaining materials from IA tissues, different data processing methods and platform conditions, the results of screening differential genes with microarray and sequencing data might vary significantly.…”