Analysis of genes and metabolites associated with propamocarb hydrochloride response in tobacco

Abstract

Propamocarb hydrochloride is a systemic fungicide used against Pythium and Phytophthora as well as other diseases in tobacco. This study aimed to understand the response of tobacco to propamocarb hydrochloride treatment at the transcriptome and metabolome levels. Transcriptomic and metabolomic analyses were conducted on tobacco leaves treated with or without propamocarb hydrochloride to identify differentially expressed genes (DEGs) and differential metabolites, which were used for further functional enrichment analyses. In all, 1364 to 2497 DEGs and 34 differential metabolites were identified in different comparable groups. The DEGs were significantly involved in functions related to photosynthesis and valine, leucine, and isoleucine degradation. Differential metabolites were mainly associated with arginine and proline metabolism (1,3-diaminopropane; 4-aminobutyric acid; and N-(omega)hydroxyarginine) and glycerophospholipid metabolism (lysoPC(16:0), phosphorylcholine, and triethanolamine). Our study identified differential genes and metabolites that were associated with the response of tobacco leaves to propamocarb hydrochloride. Propamocarb hydrochloride could influence the photosynthesis, amino acid metabolism, and glycerophospholipid metabolism of tobacco leaves.

Metabolic Study of Cucumber Seeds and Seedlings in the Light of the New, Controversial Trend of Preventive Use of Systemic Fungicides

“…Negative or beneficial physiological and biochemical changes in plants depend on the chemical structure of the applied fungicides. These changes are mostly correlated with enzyme activity or even gene expression and lead to disruption in carbon or nitrogen metabolism and photosynthesis efficiency, therefore influencing the growth and development of plants [ 15 , 16 ]. One example is propamocarb, which causes an increase in the activity of detoxification enzymes (glutathione S-transferase (E.C.…”