Analysis of Genes for Succinoyl Trehalose Lipid Production and Increasing Production in Rhodococcus sp. Strain SD-74

Inaba, Tomohiro; Tokumoto, Yuta; Miyazaki, Yusuke; Inoue, Naoyuki; Maseda, Hideaki; Nakajima-Kambe, Toshiaki; Uchiyama, Hiroo; Nomura, Nobuhiko

doi:10.1128/aem.01664-13

Cited by 35 publications

(12 citation statements)

References 33 publications

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“…A recent report proposed a hypothetical pathway for the synthesis of succinoyl trehalose lipid (STL) based on three essential genes ( fda , tlsA and alkB ) in Rhodococcus sp. SD‐74 during hexadecane degradation (Inaba et al ., ). However, TLC analysis demonstrated similar patterns of glycolipid production in wild type and the Δots A mutant strain under TRI, indicating that trehalose synthesis is not necessary for glycolipid production in the DR1 strain (Fig.…”

Section: Discussionmentioning

confidence: 97%

Metabolic and stress responses of Acinetobacter oleivorans DR1 during long‐chain alkane degradation

Park

Shin

Jung

et al. 2017

Microbial Biotechnology

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Summary Acinetobacter oleivorans DR1 can utilize C12–C30 alkanes as a sole carbon source but not short‐chain alkanes (C6, C10). Two copies of each alkB‐, almA‐ and ladA‐type alkane hydroxylase (AH) are present in the genome of DR1 cells. Expression and mutational analyses of AHs showed that alkB1 and alkB2 are the major AH‐encoding genes under C12–C30, and the roles of other almA‐ and ladA genes are negligible. Our data suggested that AlkB1 is responsible for long‐chain alkane utilization (C24–C26), and AlkB2 is important for medium‐chain alkane (C12–C16) metabolism. Phylogenetic analyses revealed large incongruities between phylogenies of 16S rRNA and each AH gene, which implies that A. oleivorans DR1 has acquired multiple alkane hydroxylases through horizontal gene transfer. Transcriptomic and qRT‐PCR analyses suggested that genes participating in the synthesis of siderophore, trehalose and poly 3‐hydroxybutyrate (PHB) were expressed at much higher levels when cells used C30 than when used succinate as a carbon source. The following biochemical assays supported our gene expression analyses: (i) quantification of siderophore, (ii) measurement of trehalose and (iii) observation of PHB storage. Interestingly, highly induced both ackA gene encoding an acetate kinase A and pta gene encoding a phosphotransacetylase suggested unusual ATP synthesis during C30 alkane degradation, which was demonstrated by ATP measurement using the ΔackA mutant. Impaired growth of the ΔaceA mutant indicated that the glyoxylate shunt pathway is important when C30 alkane is utilized. Our data provide insight into long‐chain alkane degradation in soil microorganisms.

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Section: Discussionmentioning

confidence: 97%

Metabolic and stress responses of Acinetobacter oleivorans DR1 during long‐chain alkane degradation

Park

Shin

Jung

et al. 2017

Microbial Biotechnology

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“…These considerations highlight the wide diversity in biosurfactant production mechanisms among different Rhodococcus strains and the possibility to drive the production of specific surfactant molecules by selecting the growth substrate. In this regard, supplying R. erythropolis SD-74 cultures with different n -alkanes as carbon source led to the biosynthesis of succinoyl trehalolipids featuring acyl groups of the same carbon chain length as the growth substrate (Tokumoto et al 2009 ), suggesting the possibility to direct the synthesis of specific biosurfactants by providing alkanes with specific chemical structure (Lang and Philp 1998 ; Inaba et al 2013 ; Cappelletti et al 2019a ). This property is incredibly useful for the commercial production, as most biosurfactant producers do not synthesize specifically defined derivatives.…”

Section: Biosurfactantsmentioning

confidence: 99%

Biotechnology of Rhodococcus for the production of valuable compounds

Cappelletti

Presentato

Piacenza

et al. 2020

Appl Microbiol Biotechnol

111

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Bacteria belonging to Rhodococcus genus represent ideal candidates for microbial biotechnology applications because of their metabolic versatility, ability to degrade a wide range of organic compounds, and resistance to various stress conditions, such as metal toxicity, desiccation, and high concentration of organic solvents. Rhodococcus spp. strains have also peculiar biosynthetic activities that contribute to their strong persistence in harsh and contaminated environments and provide them a competitive advantage over other microorganisms. This review is focused on the metabolic features of Rhodococcus genus and their potential use in biotechnology strategies for the production of compounds with environmental, industrial, and medical relevance such as biosurfactants, bioflocculants, carotenoids, triacylglycerols, polyhydroxyalkanoate, siderophores, antimicrobials, and metal-based nanostructures. These biosynthetic capacities can also be exploited to obtain high value-added products from low-cost substrates (industrial wastes and contaminants), offering the possibility to efficiently recover valuable resources and providing possible waste disposal solutions. Rhodococcus spp. strains have also recently been pointed out as a source of novel bioactive molecules highlighting the need to extend the knowledge on biosynthetic capacities of members of this genus and their potential utilization in the framework of bioeconomy. Key points • Rhodococcus possesses promising biosynthetic and bioconversion capacities. • Rhodococcus bioconversion capacities can provide waste disposal solutions. • Rhodococcus bioproducts have environmental, industrial, and medical relevance.

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“…Bioinformatic analysis (Table 5) allowed to detect the presence of trehalose-6-phosphate synthase (otsA) and two copies of trehalose-6-phosphate phosphatase (otsB) involved in de novo trehalose biosynthesis [39] and the genes malto-oligosyltrehalose synthase (treY), malto-oligosyltrehalose trehalohydrolase (treZ) and glycogen debranching enzyme (treX) involved in an alternative pathway for trehalose biosynthesis from maltooligosaccharides and starch or glycogen [40,41]. According to literature, two genes encoding fructose-bisphosphate aldolases, which catalyze the conversion of sugars from triose to hexose or vice versa, could play a key role in the succinic saccharide esters formation [42]. Furthermore, the genome annotation unveiled many glycosyltransferases and acyltransferases spread on several contigs, responsible for the biosynthesis of di/oligo/poly-saccharides and transfer of hydrophobic acyl groups to the sugar moiety, respectively.…”

Section: Identification Of Putative Genes Involved In Succinic Saccharide Esters Biosynthesismentioning

confidence: 99%

Combining OSMAC Approach and Untargeted Metabolomics for the Identification of New Glycolipids with Potent Antiviral Activity Produced by a Marine Rhodococcus

Esposito

Giugliano

Sala

et al. 2021

IJMS

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Natural products of microbial origin have inspired most of the commercial pharmaceuticals, especially those from Actinobacteria. However, the redundancy of molecules in the discovery process represents a serious issue. The untargeted approach, One Strain Many Compounds (OSMAC), is one of the most promising strategies to induce the expression of silent genes, especially when combined with genome mining and advanced metabolomics analysis. In this work, the whole genome of the marine isolate Rhodococcus sp. I2R was sequenced and analyzed by antiSMASH for the identification of biosynthetic gene clusters. The strain was cultivated in 22 different growth media and the generated extracts were subjected to metabolomic analysis and functional screening. Notably, only a single growth condition induced the production of unique compounds, which were partially purified and structurally characterized by liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS/MS). This strategy led to identifying a bioactive fraction containing >30 new glycolipids holding unusual functional groups. The active fraction showed a potent antiviral effect against enveloped viruses, such as herpes simplex virus and human coronaviruses, and high antiproliferative activity in PC3 prostate cancer cell line. The identified compounds belong to the biosurfactants class, amphiphilic molecules, which play a crucial role in the biotech and biomedical industry.

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Analysis of Genes for Succinoyl Trehalose Lipid Production and Increasing Production in Rhodococcus sp. Strain SD-74

Cited by 35 publications

References 33 publications

Metabolic and stress responses of Acinetobacter oleivorans DR1 during long‐chain alkane degradation

Metabolic and stress responses of Acinetobacter oleivorans DR1 during long‐chain alkane degradation

Biotechnology of Rhodococcus for the production of valuable compounds

Combining OSMAC Approach and Untargeted Metabolomics for the Identification of New Glycolipids with Potent Antiviral Activity Produced by a Marine Rhodococcus

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