Analysis of genes related to expression of aromatase and estradiol-regulated genes during sex differentiation in Xenopus embryos

Akatsuka, Naoya; Kobayashi, Hiroshi; Watanabe, Emi; Iino, Teruhiko; Miyashita, Kiichi; Miyata, Shogo

doi:10.1016/j.ygcen.2004.01.014

Cited by 9 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CRE-like sequence binds CREB or a member of the ATF family [20], and ATF-4 binds to the CREB-binding protein that interacts with CREB phosphorylated by cAMP-dependent protein kinase at Ser 133 [21]. Although the CREB/ATF family gene is activated during sex differentiation in the frog [22], no SF-1 expression is detected in the female and male gonads of the frog during the early stages of embryonic development before the onset of gonadal differentiation [23]. Therefore, if the CRE-like sequence is present in the aromatase promoter sequence of Xenopus, the CREB/ATF family must be important for the activation of aromatase gene expression and ovary-determination during sex differentiation in the Xenopus.…”

Section: Introductionmentioning

confidence: 99%

Expression of the gonadal p450 aromatase gene of Xenopus and characterization of the 5′-flanking region of the aromatase gene

Akatsuka

Komatsuzaki

Ishikawa

et al. 2005

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Expression of the gonadal p450 aromatase gene of Xenopus and characterization of the 5′-flanking region of the aromatase gene

Akatsuka

Komatsuzaki

Ishikawa

et al. 2005

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

“…For example, E 2 treatment results in functional feminization in birds (Willier,'42;Stoll et al,'82;Perrin et al,'95), amphibians (Mackenzie et al, 2003;Akatsuka et al, 2004), reptiles (Crews et al, '91;Dorizzi et al, '91;Lance and Bogart, '92;Richard-Mercier et al, '95;Wennstrom and Crews, '95;Belaid et al, 2001;Milnes et al, 2002), and teleost fishes (Piferrer, 2001;Devlin and Nagahama, 2002). Moreover, treatment with specific aromatase inhibitors, resulting in the suppression of the estrogen biosynthesis in the gonads, induces the sex reversal of genetic females to phenotypic males in many vertebrates including reptiles (Dorizzi et al,'94;Wibbels and Crews,'94), birds (Elbrecht and Smith, '92;Abinawanto et al, '96;Vaillant et al, 2001), and teleosts (Devlin and Nagahama, 2002).…”

mentioning

confidence: 99%

Characterization and expression profile of the ovarian cytochrome P‐450 aromatase (cyp19A1) gene during thermolabile sex determination in pejerrey, Odontesthes bonariensis

et al. 2007

View full text Add to dashboard Cite

Cytochrome P450 aromatase (cyp19) is an enzyme that catalyzes the conversion of androgens to estrogens and may play a role in temperature-dependent sex determination (TSD) of reptiles, amphibians, and fishes. In this study, the ovarian P450 aromatase form (cyp19A1) of pejerrey Odontesthes bonariensis, a teleost with marked TSD, was cloned and its expression profile evaluated during gonadal differentiation at feminizing (17 degrees C, 100% females), mixed-sex producing (24 and 25 degrees C, 73.3 and 26.7% females, respectively), and masculinizing (29 degrees C, 0% females) temperatures. The deduced cyp19A1 amino acid sequence shared high identity (>77.8%) with that from other teleosts but had low identity (<61.8%) with brain forms (cyp19A2), including that of pejerrey itself. The tissue distribution analysis of cyp19A1 mRNA in adult fish revealed high expression in the ovary. Semi-quantitative reverse transcription polymerase chain reaction analysis of the bodies of larvae revealed that cyp19A1 expression increased before the appearance of the first histological signs of ovarian differentiation at the feminizing temperature but remained low at the masculinizing temperature. The expression levels at mixed-sex producing temperatures were bimodal rather than intermediate, showing low and high modal values similar to those at the feminizing and masculinizing temperatures, respectively. The population percentages of high and low expression levels at intermediate temperatures were proportional to the percentage of females and males, respectively, and high levels were first observed at about the time of sex differentiation of females. These results suggest that cyp19A1 is involved in the process of ovarian formation and possibly also in the TSD of pejerrey.

show abstract

Characterization and expression pattern of two zebrafish atf7 genes

Zhao

Meng

2005

Developmental Dynamics

View full text Add to dashboard Cite

Members of the ATF/CREB (activating transcription factor/cAMP-responsive element binding protein) transcription factor family play diverse roles in controlling cell proliferation, apoptosis, and oncogenesis, as well as in embryonic development of vertebrates. We identified two zebrafish orthologs of human ATF7 gene: atf7a and atf7b. Whole-mount in situ hybridization shows that zebrafish atf7a is first expressed in the notochord precursors at 80% epiboly stage and then in the developing notochord during segmentation. The expression of atf7a is positively regulated by ntl, flh, and spr2, which are involved in development of the notochord. In contrast, atf7b is maternally expressed and during embryogenesis its mRNA is ubiquitously distributed, showing an expression pattern similar to that of mammalian Atf7. Developmental Dynamics 233: 1157-1162, 2005.

show abstract

Analysis of genes related to expression of aromatase and estradiol-regulated genes during sex differentiation in Xenopus embryos

Cited by 9 publications

References 37 publications

Expression of the gonadal p450 aromatase gene of Xenopus and characterization of the 5′-flanking region of the aromatase gene

Expression of the gonadal p450 aromatase gene of Xenopus and characterization of the 5′-flanking region of the aromatase gene

Characterization and expression profile of the ovarian cytochrome P‐450 aromatase (cyp19A1) gene during thermolabile sex determination in pejerrey, Odontesthes bonariensis

Characterization and expression pattern of two zebrafish atf7 genes

Contact Info

Product

Resources

About