Analysis of genetic diversity among different Rose (Rosa) genotypes using morphological and molecular markers (ISSR)

Abstract

Rose is a woody perennial of the genus Rosa within the family Rosaceae. In this study, phylogenetic relationship among seven different genotypes of Rose (Rosa) was determined using ISSR primers and morphological parameters. DNA was isolated using CTAB method with 0.5% of CTAB concentration. Out of 50 ISSR primers, 16 primers gave reproducible banding pattern with all the seven genotypes of Rose while others failed to produce polymorphism. A total of 108 bands were obtained with an average polymorphism of 54.69% and an average of 9.06 bands per primer. The average similarity was found to be 0.69 among all the seven genotypes using Jaccard’s similarity coefficient. On basis of polymorphism cluster analysis divided them into two major groups separating one genotype (light pink) from rest of the six genotypes and principle coordinate analysis confirmed the results. A parallel correlation was found between the morphological parameter (flower diameter) and the results of ISSR analysis as both of them divided the seven genotypes into two main groups.

Assessment of Genetic Diversity among the Elite Rose (Rosa spp.) Accessions Using Rapd Markers

“…Morphological and genetic study of the Kakinada rose revealed that it clusters with R. damascena var. Himroz, a local variation from South India [16]. A great degree of variation was found in the rose germplasm according to both morphological and molecular analyses, making it a priceless source of genetic diversity for improving roses.…”