Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers

Abstract

The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11 SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard's genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro-ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro-ecological zones, the maximum Nei's gene diversity (h = 0.2613) and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro-ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro-ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable

Morphological and genetic diversity assessment of sesame (Sesamum indicum L.) accessions differing in origin

“…Therefore, SSR markers would be one of the useful molecular markers in sesame genetic diversity analyses and in marker assisted breeding programs. Our study showed congruence with the previous studies by Surapaneni et al (2014) and Zhang et al (2010). Zhang et al (2010) reported that SSR markers are appropriate for evaluation of genetic diversities in sesame and also concluded that extensive genetic divergence existed among indigenous and exotic collections of sesame.…”

“…Our study showed congruence with the previous studies by Surapaneni et al (2014) and Zhang et al (2010). Zhang et al (2010) reported that SSR markers are appropriate for evaluation of genetic diversities in sesame and also concluded that extensive genetic divergence existed among indigenous and exotic collections of sesame. Park et al (2013) reported that 41genotype-specific alleles were identified for 12 of 14 SSR markers.…”

Agromorphological and Molecular Characterization of <i>Sesamum indicum</i> L.—An Oil Seed Crop

“…Out of the 114 scorable fragments, 97 were polymorphic revealing 85.08% polymorphism across the 33 varieties studied (Table 2). In this study primers based on GA/AG and GT/TG dinucleotides core repeats generated good profiles, which seem to indicate that the more frequent microsatellite in Sesamae contains the repeated dinucleotides (AG/GA) n and (GT/TG) n. Our results are in accordance with the SSR analysis reported earlier [18]. Baydar [27] reported that the sesame varieties with non-branching character having higher yield advantages for mechanized cultivation, because of their uniform maturity in a short growing period.…”

Assessing Genetic Variation and Relationships among a Mini Core Germplasm of Sesame (<i>Sesamum indicum</i> L.) Using Biochemical and RAPD Markers

“…Several types of molecular markers have been developed and applied to sesame genotyping, such as random amplified polymorphic DNA (RAPD) [14] [15], simple sequence repeats (SSR) [16], inter-simple se-quence repeats (ISSR) [17], amplified fragment length polymorphism (AFLP) [18], sequence-related amplified polymorphisms (SRAP) [19] and expressed sequence tags-SSR (EST-SSR).…”